| Abstract | 第1-6页 |
| 摘要 | 第6-12页 |
| Chapter 1 Introduction | 第12-32页 |
| 1 Abiotic stresses | 第12-19页 |
| 2 Abiotic stress-inducible genes | 第19-20页 |
| 3 Role of transcription factors in heat stress responses | 第20-21页 |
| 4 cis-acting regulatory elements | 第21-22页 |
| 5 Heat shock proteins/molecular chaperones | 第22页 |
| 6 Promoters | 第22-23页 |
| 7 Promoters in Transgenic rice | 第23-25页 |
| 8 Reporter Gene | 第25-26页 |
| 9 DNA Microarray Technology | 第26-27页 |
| 10 Transcript Profiling and the response mechanisms under heat stress | 第27-31页 |
| 11 The Objective of this research | 第31-32页 |
| Chapter 2 Microarray data uncover the genome-wide response to heat in rice post-meiosispanicle | 第32-62页 |
| 1 Materials and methods | 第32-37页 |
| ·Plant materials and heat treatments | 第32-33页 |
| ·RNA isolation and Purification for cDNA Microarray | 第33-34页 |
| ·Microarray Hybridization, Signal Scanning and Normalization | 第34-36页 |
| ·Data Filtering, Clustering and Functional Classification | 第36-37页 |
| ·qRT-PCR Analysis | 第37页 |
| 2 Result | 第37-59页 |
| ·Replicate analysis and quantitative real-time PCR (qRT-PCR) confirmation | 第37-40页 |
| ·Expression pattern of deferentially expressed genes during heat treatment | 第40-41页 |
| ·Functional identification of the heat-responsive (HR) genes | 第41-43页 |
| ·Cellular response | 第43-50页 |
| ·Regulation-related genes | 第50-58页 |
| ·Metabolism-related genes | 第58-59页 |
| 3 Discussion | 第59-62页 |
| Chapter 3 Expression Analysis of Six Heat Stress-inducible Genes in Rice | 第62-73页 |
| 1 Materials and methods | 第62-66页 |
| ·Identification of candidate stress inducible genes from microarray data | 第62页 |
| ·Plants Materials, Growth Condition | 第62-63页 |
| ·RNA Isolation and quantitative RT-PCR analysis | 第63-66页 |
| 2 Result | 第66-71页 |
| ·Expression of six selected rice genes under abiotic stresses as revealed by Microarray data | 第66-68页 |
| ·Expression pattern of the six selected genes in different tissues | 第68页 |
| ·Expression patterns of the six genes under abiotic stresses and the ABA treatment validated by quantitative RT-PCR | 第68-71页 |
| 3 Discussion | 第71-73页 |
| Chapter 4 Expression Analysis of Abiotic Stress-inducible Promoters in Rice | 第73-83页 |
| 1 Materials and methods | 第73-77页 |
| ·Bioinformatics analysis of the promoters of three inducible genes | 第73页 |
| ·Plasmid construction for the three stress-inducible promoters | 第73-74页 |
| ·Plant Transformation | 第74-75页 |
| ·Polymerase chain reaction (PCR) screening on transgenic rice | 第75页 |
| ·Heat and Drought treatments on transgenic rice lines | 第75页 |
| ·GUS gene expression analysis by qRT-PCR | 第75-76页 |
| ·Histochemical GUS Staining | 第76页 |
| ·Quantitative GUS activity assay | 第76-77页 |
| 2 Result | 第77-82页 |
| ·Bioinformatics analysis on the promoters of three stress-inducible genes | 第77页 |
| ·Confirmation of the stress-inducible promoter:GUS recombinants through PCR | 第77-78页 |
| ·Confirmation of the T2 transgenics through PCR | 第78-79页 |
| ·Expression of GUS gene driven by the three stress-inducible promoters in transgenic rice | 第79-82页 |
| 3 Discussion | 第82-83页 |
| Chapter 5 Conclusion, Innovation and Future Research | 第83-86页 |
| 1 Conclusion | 第83-84页 |
| 2 Innovation | 第84-85页 |
| 3 Future Research | 第85-86页 |
| References | 第86-103页 |
| Acknowledgments | 第103-104页 |
| About the Author | 第104页 |
