| 摘要 | 第1-14页 |
| Abstract | 第14-18页 |
| Chapter 1 Introduction | 第18-22页 |
| Chapter 2 Review of Literature | 第22-49页 |
| ·Effect of Elevated CO_2 on Plants Physiology | 第22-31页 |
| ·Effect on growth of plants | 第22-24页 |
| ·Prolonged exposure to high [CO_2] and photosynthetic acclimation | 第24-25页 |
| ·Carbohydrate source-sink balance and regulation of photosynthesis under high [CO_2] | 第25-26页 |
| ·Elevated [CO_2] and N supply | 第26-27页 |
| ·Elevated [CO_2] and stomatal behavior | 第27-28页 |
| ·Effect of elevated [CO_2] on respiration | 第28-30页 |
| ·Elevated [CO_2] and lipid metabolism | 第30页 |
| ·Proteomic study on elevated [CO_2] | 第30-31页 |
| ·Rice Proteomics | 第31-45页 |
| ·Rice leaf proteomics | 第32-36页 |
| ·Differential proteomics of rice leaves | 第36-45页 |
| ·Apoplast Proteome | 第45-49页 |
| Chapter 3 Materials and Methods | 第49-58页 |
| ·Chemicals | 第49页 |
| ·Seedling Growth and Treatment | 第49-50页 |
| ·Physiological Measurements and Sample Collection | 第50页 |
| ·Protein Extraction and Sample Preparation | 第50-55页 |
| ·Total leaf proteins | 第50-51页 |
| ·Apoplast proteins | 第51-53页 |
| ·Glucose -6-phosphate dehydrogenase (G6PDH) assay | 第53-54页 |
| ·-DE Analysis | 第54-55页 |
| ·Gel Scanning and Image Analysis | 第55页 |
| ·In-gel Digestion | 第55-56页 |
| ·Protein Identification | 第56-58页 |
| Chapter 4 Proteomic Response of Rice Seedling Leaves to Elevated CO_2 | 第58-82页 |
| ·Physiological Responses Elicited by Elevated CO_2 | 第58-61页 |
| ·2-DE Analysis Reveals 57 Differential Proteins in Response to High CO_2 | 第61-67页 |
| ·Some Differential Proteins Show Specific Expression Patterns | 第67-71页 |
| ·The Proteins Showing Maximal Change at a Given [CO_2]May Contribute to Sustain the Given Physiological State | 第71-73页 |
| ·At 760 ppm (2x) [CO_2] | 第71-72页 |
| ·At 1140 ppm (3x) [CO_2] | 第72-73页 |
| ·At 1520 ppm (4x) [CO_2] | 第73页 |
| ·Sequential Rise in [CO_2] Shuffles the Photosynthetic Proteome. | 第73-74页 |
| ·Pn and/or Gs Down Regulation is Modulated by Changes in the Calvin Cycle Enzymes | 第74-76页 |
| ·Several Proteins Involved in Protein Processing Respond to High CO_2 | 第76-77页 |
| ·Induction of Enzymes of the Energy Pathway Alludes to Higher Energy Demand | 第77-78页 |
| ·C2-Domain Containing Protein May Be Involved in Transducing Signals for Elevated CO_2 | 第78页 |
| ·Does a Maximal Increase in Pn Result in Induction of APX? | 第78-79页 |
| ·Concluding Remarks | 第79-82页 |
| Chapter 5 Apoplast Protein Extraction and 2-DE Analysis | 第82-94页 |
| ·Number and 2-DE Pattern of Proteins Depends on the Nature and Composition of the Buffer Used | 第84-85页 |
| ·Buffer B is Most Appropriate for Rice Root and Leaf Apoplast Proteins Extraction and 2-DE | 第85-90页 |
| ·Technical Bottlenecks of the Apoplast Extraction | 第90-91页 |
| ·Sample extraction | 第90-91页 |
| ·-DE analysis | 第91页 |
| ·Possible Ways Out | 第91-92页 |
| ·Concluding Remarks | 第92-94页 |
| Overall Conclusions | 第94-96页 |
| References | 第96-113页 |
| Appendix 1 | 第113-116页 |
| Appendix 2 | 第116-120页 |
| Acknowledgement | 第120-121页 |
