| Contents | 第1-5页 |
| Abbreviations | 第5-6页 |
| 中文摘要 | 第6-8页 |
| Abstract | 第8-11页 |
| Main text | 第11-67页 |
| 1.Introduction | 第11-17页 |
| 2.Materials and methods | 第17-23页 |
| ·Retrograde fluorescent labeling of the preganglionic cardiac vagal neurons(the CVNs) | 第17页 |
| ·Slice prepara tion | 第17-18页 |
| ·Electrophysiological recording | 第18-19页 |
| ·Identification of the CVNs | 第18页 |
| ·Recording of the glutamatergic spontaneous excitatory postsynaptic currents(sEPSCs)with the K~+ gluconate-dominated pipette solution | 第18-19页 |
| ·Recording of the spontaneous or the miniature inhibitory postsynaptic currents(sIPSCs or mIPSCs)with the KCl-dominated pipette solution | 第19页 |
| ·Measurement of input resistance | 第19页 |
| ·Recording of respiratory-related hypoglossal signal | 第19页 |
| ·Drugs and experimental protocols | 第19-21页 |
| ·Data collection and analysis | 第21-23页 |
| 3.Results | 第23-49页 |
| ·Distribution of the fluorescence-labeled neurons in the NA and functional identification of the CVNs | 第23-26页 |
| ·Effects of the glutamatergic neurotransmission on the GABAergic inputs of the CVNs | 第26-31页 |
| ·Effect of CNQX and AP_5 on the frequency of the GABAergie sIPSCs of the CVNs in the dorsal motor nucleus of the vagus(DMNV) | 第26-28页 |
| ·Effect of CNQX and AP_5 on the frequency of the GABAergic sIPSCs of the CVNs in the nucleus ambiguus(NA) | 第28-31页 |
| ·Effects of inhibitory neurotransmission on the glutamatergic inputs of the CVNs in the NA | 第31-39页 |
| ·Combined application of picrotoxin and strychnine evoked seizure-like activation of both the CVNs and the hypoglossal motor neurons--experiments in slices with respiratory-like rhythm | 第31-36页 |
| ·Combined application of picrotoxin and strychnine,but not depiction of MgCl_2,elevation of KCl concentration,or increase of temperature,evoked seizure-like activation of the CVNs and the hypoglossal motor neurons--experiments in slices without respi | 第36-39页 |
| ·Modulatory effects of orexin-A on the amino acidergic inputs of the CVNs in the NA | 第39-42页 |
| ·Responses of the glycinergic sIPSCs to different doses of orexin-A | 第39-40页 |
| ·Responses of the GABAergic sIPSCs to different doses of orexin-A | 第40-42页 |
| ·Modulatory effects of substance P(SP)on the amino acidergic inputs of the CVNs in the NA | 第42-49页 |
| ·Effects of SP on the inhibitory inputs of the CVNs | 第42-45页 |
| ·Effects of SP on the excitatory inputs of the CVNs | 第45-46页 |
| ·Effects of SP on the inspiratory-related augmentation of the GABAergic and the glycinergic sIPSCs of the CVNs | 第46-49页 |
| 4.Discussion | 第49-57页 |
| ·Effects of the glutamatergic neurotransmission on the GABAergic inputs of the CVNs | 第49页 |
| ·Effect of inhibitory neurotransmission on the glutamatergic inputs of the CVNs | 第49-52页 |
| ·Modulation of the amino acidergic inputs of the CVNs in the NA by orexin-A | 第52页 |
| ·Modulation of the amino acidergic inputs of the CVNs in the NA by SP | 第52-55页 |
| ·About the identification of the CVNs | 第55-57页 |
| 5.Summary of results and conclusions | 第57-58页 |
| 6.References | 第58-66页 |
| 7.Acknowledgements | 第66-67页 |
| Appendix 1:Publications and submissions during doctoral study | 第67-68页 |
| Appendix 2:Literature Review:心脏副交感节前神经元 | 第68-81页 |
