| 摘要 | 第6-7页 |
| ABSTRACT | 第7页 |
| CHAPTER Ⅰ INTRODUCTION AND REVIEW OF LITERATURE | 第16-46页 |
| 1.1 Microbial Biocontrol Agents | 第16-40页 |
| 1.1.1 Entomopathogenic Viruses | 第16-17页 |
| 1.1.2 Entomopathogenic Bacteria | 第17页 |
| 1.1.3 Entomopathogenic Nematodes | 第17页 |
| 1.1.4 Entomopathogenic Fungi | 第17-33页 |
| 1.1.5 The Fungal Breaching Process | 第33-38页 |
| 1.1.6 Host Defense Systems | 第38-39页 |
| 1.1.7 Advantages of Using Fungi as Insecticides | 第39-40页 |
| 1.1.8 Disadvantages of the Fungi as Biocontrol Agents | 第40页 |
| 1.2 Plant Defense Responses | 第40-41页 |
| 1.3 Elicitors Induce Resistance | 第41-42页 |
| 1.4 Chinese Cabbage Defense Response | 第42-43页 |
| 1.5 Abiotic and Biotic Stresses Related Pathways | 第43-45页 |
| 1.5.1 Jasmonic Acid Signaling Pathway | 第43-44页 |
| 1.5.2 Ethylene Signaling Pathway | 第44-45页 |
| 1.6 Objectives | 第45-46页 |
| CHAPTER Ⅱ In vitro Pathogenicity of Some Entomopathogenic Fungal Strains against Green Peach Aphid Myzus persicae(Homoptera:Aphididae) | 第46-57页 |
| 2.1 Introduction | 第46-47页 |
| 2.2 Materials and Methods | 第47-49页 |
| 2.2.1 Insect Culture | 第47页 |
| 2.2.2 Fungal Isolates and Conidial Suspensions Preparation | 第47-48页 |
| 2.2.3 Pathogenicity Bioassays | 第48页 |
| 2.2.4 Data Analysis | 第48-49页 |
| 2.3 Results | 第49-55页 |
| 2.3.1 Pathogenicity of Filtrates by Different Strains of B.bassiana and L.lecanii against M.persicae | 第49-51页 |
| 2.3.2 Virulence of Conidial Bioassay of Three Fungal Strains against M.persicae | 第51-53页 |
| 2.3.3 Entomopathogenicity of Binary Combinations of Entomopathogenic Fungal Strains against M.persicae | 第53-55页 |
| 2.4 Discussion | 第55-56页 |
| 2.5 Summary | 第56-57页 |
| CHAPTER Ⅲ PeBb1,a Novel Protein Elicitor from Beauveria bassiana Strain ARSEF2860,Induces Systemic Resistance in Brassica rapa subsp.pekinensis against Myzus persicae(Homoptera:Aphididae) | 第57-77页 |
| 3.1 Introduction | 第57-59页 |
| 3.2 Materials and Methods | 第59-67页 |
| 3.2.1 Rearing of Myzus persicae | 第59页 |
| 3.2.2 Plant,Pathogen and Bacteria Culture | 第59页 |
| 3.2.3 Isolation and Detection of Crude Protein | 第59-60页 |
| 3.2.4 Purification of Protein | 第60页 |
| 3.2.5 Mass Spectrometry Analysis and Gene Cloning | 第60-64页 |
| 3.2.6 Expression and Purification of Recombinant Protein | 第64-65页 |
| 3.2.7 Characterization of the PeBb1 Protein Elicitor | 第65页 |
| 3.2.8 Bioassay of Elicitor Activity against M.persicae | 第65页 |
| 3.2.9 Analysis of the Expression of Defense-related Genes Induced by PeBb1 using RT-qPCR | 第65-67页 |
| 3.2.10 Protein Assay | 第67页 |
| 3.2.11 Statistical Analysis | 第67页 |
| 3.3 Results | 第67-74页 |
| 3.3.1 Purification,Characterization and Identification of PeBb | 第67页 |
| 3.3.2 PeBb1 Induced Hypersensitive Response HR in Tobacco Leaves | 第67-70页 |
| 3.3.3 Effect of Elicitor PeBb1 on the Fecundity of M.persicae | 第70-72页 |
| 3.3.4 Expression Level of Plant Defense-Associated Genes in Response to PeBb1 elicitor | 第72-74页 |
| 3.4 Discussion | 第74-76页 |
| 3.5 Summary | 第76-77页 |
| CONCLUSION | 第77-78页 |
| REFERENCES | 第78-106页 |
| ACKNOWLEDGEMENTS | 第106-107页 |
| RESUME | 第107-110页 |
