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Genome-Wide Association Mapping for Seed Dormancy and Molecular Cloning and Characterization of OsDOG1,A Rice Seed Dormancy Candidate Gene

摘要第7-9页
ABSTRACT第9-10页
ABBREVIATIONS USED IN THIS STUDY第11-12页
CHAPTERⅠ第12-42页
    1.LITERATURE REVIEW第12-42页
        1.1 SEED DORMANCY第12-13页
        1.2 RELATIONSHIP BETWEEN SEED DORMANCY AND GERMINATION第13-15页
            1.2.1 Parameters of germination assays第14页
            1.2.2 Primary Dormancy and Secondary Dormancy第14-15页
        1.3 CLASSIFICATION SYSTEM FOR SEED DORMANCY第15-18页
            1.3.1 Physiological dormancy (PD)第15-16页
            1.3.2 Morphological dormancy (MD)第16-17页
            1.3.3 Morphophysiological dormancy (MPD)第17页
            1.3.4 Physical Dormancy (PY)第17页
            1.3.5 Combinational dormancy (PY+PD)第17-18页
        1.4 SEED STRUCTURE AND SEED DORMANCY EVOLUTION第18-21页
        1.5 HORMONAL CONTROL OF SEED DORMANCY第21-27页
            1.5.1 Abscisic Acid (ABA)第22-24页
            1.5.2 Gibbererellins (GA)第24页
            1.5.3 Brassinosteroids (BR)第24-25页
            1.5.4 Ethylene第25-26页
            1.5.5 Nitrogen Containing compounds and Active Oxygen Species第26-27页
        1.6 CONTROL OF DORMANCY AND GERMINATION BY ENVIRONMENT AND SEED COAT COMPONENTS第27-30页
            1.6.1 Seed coat components第27-28页
            1.6.2 Dormancy Release by After-Ripening (AR)第28-29页
            1.6.3 Dormancy Control by Light and Temperature第29-30页
        1.7 REGULATION OF SEED DORMANCY第30-34页
        1.8 QTL ANALYSIS OF SEED DORMANCY IN RICE第34-36页
        1.9 MARKER ASSISTED SELECTION FOR YIELD IMPROVEMENT IN RICE第36-38页
        1.10 GENOME-WIDE ASSOCIATION STUDIES (GWAS) IN RICE第38-42页
CHAPTER Ⅱ GENOME-WIDE ASSOCIATION MAPPING REVEALED A DIVERSE GENETICBASIS OF SEED DORMANCY ACROSS SUBPOPULATIONS IN RICE (Oryza sativa L.)第42-76页
    1. METHODS第43-52页
        1.1 Plant materials and sequencing第43-45页
        1.2 Collection of sequence data,sequence alignment and SNP identification第45-46页
        1.3 SNP annotation第46页
        1.4 QTL Imputing missing genotype using an LD-KNN algorithm第46-47页
        1.5 Estimating the accuracy of genotyping and imputation and the sensitivity of SNPidentification第47页
        1.6 Field Experiments第47-48页
        1.7 Phenotype assessment for seed dormancy第48-49页
        1.8 Statistical Analysis第49-50页
        1.9 Association mapping第50-51页
        1.10 Sdr4 and GA2ox3 Haplotype analysis第51-52页
    2. RESULTS第52-69页
        2.1 Phenotypic evaluations and heritability第52-57页
        2.2 Association mapping in FHS第57-60页
        2.3 Association mapping in ARS第60-64页
        2.4 Genes and QTL around the putative peak positions第64-65页
        2.5 Sdr4 haplotypes analysis第65-67页
        2.6 GA2OX3 haplotypes analysis第67-69页
    3. DISCUSSION第69-76页
        3.1 Diverse genetic basis of seed dormancy in indica,japonica and Aus subpopulations第69-71页
        3.2 Early and late detectable signals controlling seed dormancy第71-72页
        3.3 Candidate genes for seed dormancy第72-74页
        3.4 Sdr4 and GA2ox3 haplotypes for breeding pre-harvest sprouting resistance variety第74-76页
CHAPTER Ⅲ MOLECULAR CLONING AND CHARACTERIZATION OF(OSDOG1),A RICE SEED DORMANCY CANDIDATE GENE第76-89页
    1. METHODS第77-83页
        1.1 Plant Materials第77页
        1.2 DNA extraction第77-78页
        1.3 RNA extraction第78页
        1.4 Complementary DNA(cDNA)synthesis and RT-PCR第78-79页
        1.5 Cloning of OsDOG1,OsDOG2 and OsDOG3第79-80页
        1.6 Transformation of OsDOG1 into Nipponbare第80页
        1.7 Germination Testing第80-81页
        1.8 ABA Measurements第81页
        1.9 UFLC-ESI-MS/MS第81-82页
        1.10 Gene expression analyses第82-83页
    2. RESULTS AND DISCUSSION第83-89页
        2.1 Germination Characteristics第83-85页
        2.2 ABA quantification第85-86页
        2.3 Genes involved in ABA Biosynthesis pathway第86-89页
CONCLUSION第89-91页
REFERENCES第91-111页
ACKNOWLEDGEMENTS第111-113页
PERSONAL INFORMATION第113页
PUBLICATIONS第113-114页
SCHOLARSHIP AWARDS第114页

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