| TABLE OF CONTENTS | 第5-9页 |
| ACKNOWLEDGEMENT | 第9-11页 |
| ABSTRACT | 第11-13页 |
| ABBREVIATIONS | 第14-16页 |
| CHAPTER-1:REVIEW OF LITERATURES | 第16-34页 |
| 1.1 General Introduction for Mushroom Science Research | 第16-18页 |
| 1.1.1 Edible and Medicinal Mushroom | 第16-18页 |
| 1.1.2 Poisonous Mushroom | 第18页 |
| 1.2 Life Cycle and Genetics of Agaricus bisporus | 第18-31页 |
| 1.2.1 Characteristics of A. bisporus | 第21-22页 |
| 1.2.2 Thallisms in Sexual Reproduction of Mushroom | 第22-23页 |
| 1.2.3 Genome and Fruit-body Development of A. bisporus | 第23-26页 |
| 1.2.4 Application of Molecular Marker in A. bisporus | 第26-31页 |
| 1.3 Differentiation of Homokaryon and Heterokaryon | 第31-33页 |
| 1.4 Objectives of this Project | 第33-34页 |
| CHAPTER-2:MATERIALS AND METHODS | 第34-64页 |
| 2.1 Test Strains and Its Sources | 第34页 |
| 2.2 Isolation of Single Basidiospore and Primary Selection of Putative Homokaryon | 第34-37页 |
| 2.2.1 Basidiospore Collection | 第34-35页 |
| 2.2.2 Basidiospore Germination and Isolation of Single Spore Culture | 第35页 |
| 2.2.3 Primary Screening of Homokaryotic Isolates | 第35-36页 |
| 2.2.4 Cross Fertility Test of Single Spore Isolates | 第36-37页 |
| 2.3 Protoplast Preparation and Primary Selection of Putative Homokaryotic Protoplasts | 第37-44页 |
| 2.3.1 Preparation of Mycelia Materials for Protoplast Isolation | 第38-39页 |
| 2.3.2 Procedure of Protoplast Preparation | 第39-42页 |
| 2.3.3 Protoplast Regeneration and Reversion | 第42页 |
| 2.3.4 Primary Screening of Homokaryotic Protoclones | 第42-43页 |
| 2.3.5 Cross Fertility Test of Protoclones | 第43-44页 |
| 2.4 Cultivation of Putative Homokaryotic Strains | 第44-46页 |
| 2.4.1 Spawn Preparation | 第44-46页 |
| 2.4.2 Cultivation | 第46页 |
| 2.5 DNA Polymorphism Analysis of Single Spore Isolates and Protoclones | 第46-62页 |
| 2.5.1 Genomic DNA Extraction | 第46-54页 |
| 2.5.2 Electrophoresis | 第54-56页 |
| 2.5.3 PCR Amplification | 第56-62页 |
| 2.6 Cross Breeding and Preliminary Selection of Hybrids | 第62-63页 |
| 2.6.1 Monokaryotic Parental Strains | 第62页 |
| 2.6.2 Mating Reaction | 第62-63页 |
| 2.7 Statistical Analysis of Data | 第63-64页 |
| CHAPTER-3:RESULTS AND ANALYSIS | 第64-90页 |
| 3.1 Screening of Putative Homokaryons from Single Spore Isolates | 第64-65页 |
| 3.2 Screening of Putative Homokaryons from Protoplast Regenerates | 第65-66页 |
| 3.3 Characteristics of Putative Homokaryons | 第66-70页 |
| 3.4 Fruiting Trial for Homokaryon Differentiation | 第70-71页 |
| 3.5 Cross Fertility Test | 第71-74页 |
| 3.6 DNA Polymorphism Analysis | 第74-85页 |
| 3.6.1 ISSR Analysis of 15 Putative homokaryotic SSIs | 第74-77页 |
| 3.6.2 ISSR Analysis of 30 Discarded SSIs | 第77-78页 |
| 3.6.3 RAPD Analysis of 9 Putative Homokaryotic Protoclones | 第78-80页 |
| 3.6.4 ISSR analysis of 9 Putative Homokaryotic Protoclones | 第80-83页 |
| 3.6.5 Combined RAPD and ISSR Analysis of 9 Putative Homokaryotic Protoclones | 第83-85页 |
| 3.7 Hybrids Identification and Cultivation | 第85-90页 |
| 3.7.1 Colony Morphology | 第85-86页 |
| 3.7.2 Cultivation of Hybrid Strains | 第86页 |
| 3.7.3 Identification of Hybrids by Molecular Markers | 第86-90页 |
| CHAPTER-4:DISCUSSION | 第90-96页 |
| 4.1 General Discussion | 第90-91页 |
| 4.2 Single Spore Homokaryons Differentiation and Identification | 第91-92页 |
| 4.3 Characteristics of Discarded Single Spore Isolates | 第92页 |
| 4.4 Protoplast Homokaryons Differentiation and Identification | 第92-93页 |
| 4.5 ISSR for Single Spore Isolates and Protoclones | 第93-94页 |
| 4.6 Hybrid Heterokaryons | 第94-96页 |
| CHAPTER-5: REFERANCES | 第96-109页 |
| RESUME | 第109-120页 |
