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玉米耐盐性的鉴定及QTL分析

摘要第6-7页
Abstract第7-8页
CHAPTER 1. GENERAL INTRODUCTION第18-47页
    1.1 Salinity stress:a major threat to global food security第18-19页
    1.2 Extent and distribution of soil salinity第19页
    1.3 Plant responses to salinity stress第19-22页
    1.4 Salt sensitivity in relation to developmental growth stage第22-25页
        1.4.1 Germination and seedling emergence第22-23页
        1.4.2 Vegetative growth第23-24页
        1.4.3 Roots第24页
        1.4.4 Shoots第24-25页
        1.4.5 Reproductive growth第25页
    1.5 Responses of maize to salt stress第25-26页
    1.6 Plants vary in tolerance第26页
    1.7 Mechanisms of salt tolerance第26-29页
        1.7.1 Ion exclusion第27页
        1.7.2 Ion compartmentation第27-29页
        1.7.3 Osmotic adjustment第29页
    1.8 Molecular control of salt tolerance第29-31页
    1.9 Improvement of salt tolerance in cereals第31-44页
        1.9.1 Plant phenotyping第31-32页
        1.9.2 Screening criteria for salt tolerance第32-34页
        1.9.3 Evaluation of salt tolerance at germination stage第34页
        1.9.4 Evaluation of salt tolerance at vegetative stage in hydroponic第34-36页
        1.9.5 Evaluation of salt tolerance in field conditions第36-37页
        1.9.6 Diallel crossing and genetic basis of salt tolerance第37-39页
        1.9.7 Molecular markers第39-41页
        1.9.8 DNA fingerprinting第41页
        1.9.9 Marker assisted selection第41-42页
        1.9.10 QTLAnalysis第42-44页
    1.10 Molecular genetic mapping in maize第44-45页
    1.11 Sequencing of Maize Genome第45-46页
    1.12 Objectives第46-47页
CHAPTER 2. Evaluation of Maize Salinity Tolerance at Seed Germination Stage第47-59页
    2.1 Summary第47页
    2.2 Introduction第47-50页
    2.3 Materials and Methods第50-51页
        2.3.1 Plant Materials第50页
        2.3.2 Methods第50-51页
    2.4 Statistical Analysis第51-53页
    2.5 Results第53-57页
    2.6 Discussion第57-58页
    2.7 Conclusion第58-59页
CHAPTER 3. Evaluation of Maize Salinity Tolerance at Seedling Stage第59-74页
    3.1 Summary第59页
    3.2 Introduction第59-61页
    3.3 Materials and Methods第61-62页
        3.3.1 Materials第61页
        3.3.2 Plant growth conditions第61页
        3.3.3 Method 1:Solution-culture(Hydroponic)第61页
        3.3.4 Method 2:Vermiculite-culture第61-62页
    3.4 Statistical analysis第62页
    3.5 Results第62-65页
        3.5.1 Effect of salt stress on shoot length第62-63页
        3.5.2 Effect of salt stress on root length第63页
        3.5.3 Total number of leaf reduces due to effect of salt stress第63页
        3.5.4 Biomass reduction due to salt stress第63-65页
        3.5.6 Effects of salt stress on Na~+and K~+ uptake第65页
    3.6 Discussion第65-73页
        3.6.1 Effects of salt stress on physiological traits第66-72页
        3.6.2 Effects of salt stress on nutrient uptake第72-73页
        3.6.3 Evaluation methods for salt screening第73页
    3.7 Conclusion第73-74页
CHAPTER 4. Mapping QTLs of Maize Salinity Tolerance at Seedling Stage第74-92页
    4.1 Summary第74页
    4.2 Introduction第74-76页
    4.3 Materials and Methods第76-77页
        4.3.1 Populations used for QTL analysis第76页
        4.3.2 Experimental design第76页
        4.3.3 Trait measurement第76页
        4.3.4 Molecular analysis第76-77页
        4.3.5 Linkage map construction第77页
    4.4 Statistical analysis and QTL mapping第77页
        4.4.1 Phenotypic data analysis第77页
        4.4.2 QTL Mapping第77页
    4.5 Results第77-84页
        4.5.1 Phenotypic evaluation第77-78页
        4.5.2 QTL analysis第78页
        4.5.3 QTLs for shoot length第78-79页
        4.5.4 QTLs for root length第79页
        4.5.5 QTLs for ration of root length and shoot length第79-80页
        4.5.6 QTLs for shoot fresh weight第80页
        4.5.7 QTLs for root fresh weight第80页
        4.5.8 QTLs for plant fresh weight第80-81页
        4.5.9 QTLs for shoot dry weight第81页
        4.5.10 QTLs for root dry weight第81页
        4.5.11 QTLs for plant dry weight第81页
        4.5.12 QTLs for ratio of root dry weight and shoot dry weight第81-84页
    4.6 Discussion第84-91页
    4.7 Conclusion第91-92页
CHAPTER 5. GENERAL DISCUSSION AND CONCLUSION第92-97页
    5.1 Salt tolerance screening strategies第92-94页
    5.2 Bi-parental QTL mapping for salt tolerance第94-95页
    5.3 QTL mapping of physiological traits associated with salt tolerance in maize第95页
    5.4 General conclusion第95-97页
REFERENCES第97-126页
ACKNOWLEDGEMENTS第126-128页
RESUME第128-132页
APPENDIX第132-137页

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