| Abbreviations | 第5-9页 |
| 摘要 | 第9-11页 |
| Abstract | 第11-13页 |
| Introduction | 第14-16页 |
| Chapter 1 Literature Review | 第16-33页 |
| 1.1 Tillering development in rice | 第16-20页 |
| 1.1.1 Initiation of tillers in rice | 第17页 |
| 1.1.2 Factors influencing tiller development | 第17页 |
| 1.1.3 Hormones controlling tillering in rice | 第17-19页 |
| 1.1.4 Genes regulating tillering and branching | 第19-20页 |
| 1.2 Flower Development in Rice | 第20-24页 |
| 1.2.1 ABC model | 第21-22页 |
| 1.2.2 Lemma and palea morphology | 第22-23页 |
| 1.2.3 Lodicule morphology | 第23页 |
| 1.2.4 Gynoecium sterility | 第23-24页 |
| 1.2.5 Grain size | 第24页 |
| 1.3 Meristem Development in Rice | 第24-29页 |
| 1.3.1 Wuschel-related homeobox (WOX) genes | 第25-27页 |
| 1.3.2 CLAVATA-WUSCHEL pathway | 第27-29页 |
| 1.3.3 WUSCHEL and AGAMOUS interactions in floral meristems | 第29页 |
| 1.4 Arginine | 第29-31页 |
| 1.5 Bulked segregant analysis (BSA) and Whole Genome Sequencing | 第31-33页 |
| Chapter 2 Identification and Cloning of Sterile and Reduced Tillering 1 in Rice | 第33-54页 |
| 2.1 Materials and Methods | 第34-37页 |
| 2.1.1 Plant materials and field experiments | 第34页 |
| 2.1.2 Fast mapping of SRT1 using bulked segregant analysis by sequencing (BSA-seq) | 第34-35页 |
| 2.1.3 Fine mapping of SRTl | 第35-36页 |
| 2.1.4 Pollen viability assay | 第36页 |
| 2.1.5 Multiple sequence alignment | 第36-37页 |
| 2.1.6 Protein 3D modeling | 第37页 |
| 2.1.7 Constructs of GUS (β-glucuronidase) Expressing System, Transformation, and GUS staining | 第37页 |
| 2.2 Results | 第37-48页 |
| 2.2.1 Greatly reduced number of tillers in srtl | 第37-40页 |
| 2.2.2 Complete female sterility in srtl | 第40-41页 |
| 2.2.3 A recessive sporophytic mutation | 第41-42页 |
| 2.2.4 Co-segregation of sterility and tillering defects in srt1 | 第42页 |
| 2.2.5 Mapping of SRT1 | 第42-44页 |
| 2.2.6 Identification of srt1 as LOC_Os04g56780 | 第44-45页 |
| 2.2.7 Mutation in homeobox of OsWUS | 第45-46页 |
| 2.2.8 Expression Patterns of OsWUS | 第46-48页 |
| 2.3 Discussion | 第48-54页 |
| 2.3.1 SRT1 controls tillering and panicle branching in rice | 第48-49页 |
| 2.3.2 Mutation in srtl results in complete female sterility | 第49-51页 |
| 2.3.3 SRT1 influence the spikelet development | 第51页 |
| 2.3.4 The function of homeobox in OsWUS | 第51-54页 |
| Chapter 3 Identification and Cloning of Pepper-Shaped Husk 1 in Rice | 第54-69页 |
| 3.1 Materials and Methods | 第55-58页 |
| 3.1.1 Plant Materials and phenotype observation | 第55页 |
| 3.1.2 Pollen viability assay | 第55页 |
| 3.1.3 Mapping of PSH1 using BSA sequencing method | 第55-56页 |
| 3.1.4 Fine Mapping of the PSH1 | 第56-57页 |
| 3.1.5 Detection of Oxidative Stress | 第57-58页 |
| 3.2 Results | 第58-65页 |
| 3.2.1 psh1 mutant produces pepper-shaped spikelet | 第58-59页 |
| 3.2.2 Abnormal grain size and shape of psh1-1 | 第59-60页 |
| 3.2.3 Genetic analysis of psh1-1 | 第60-61页 |
| 3.2.4 Mapping of the PSH1 | 第61-62页 |
| 3.2.5 Identification of PSH1 | 第62-63页 |
| 3.2.6 PSH1-1/2 are new alleles of OsARG | 第63-64页 |
| 3.2.7 PSH1-1/2 possible effects of the Arginine Catabolic failure | 第64-65页 |
| 3.3 Discussion | 第65-69页 |
| 3.3.1 Does OsARG regulate the development of lemma and palea in psh1-1/2? | 第65-66页 |
| 3.3.2 OsARG may regulate the flower opening in psh1-1/2 | 第66-67页 |
| 3.3.3 Possible Effects on Failure of Arginase Activity | 第67-68页 |
| 3.3.4 Presence of Two AHele Confirms the Gene | 第68-69页 |
| References | 第69-79页 |
| Appendix | 第79-89页 |
| 1 DNA Extraction with CTAB Method | 第79页 |
| 2 DNA Extraction with SDS Method | 第79-80页 |
| 3 Detection of DNA using Agarose Gel | 第80-81页 |
| 4 Resin Sectioning | 第81-82页 |
| 5 GUS Transformation | 第82-85页 |
| 6 Detection of Oxidative Stress | 第85-86页 |
| 7 Detection of Superoxide Anion using ftO-soluble Tetrazolium salt | 第86页 |
| 8 Detection of Hydrogen Peroxide using Potassium Titanium (Ⅳ) Oxalate | 第86页 |
| 9 OsWUS Structure & Genomic Sequence | 第86-87页 |
| 10 OsArginase Structure & Genomic Sequence | 第87-89页 |
| Publication(s) | 第89-90页 |
| Acknowledgement | 第90页 |
