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Analysis of Temperature Effects on Rice Flowering and Yield Traits Through QTL Approach and Identification of a Novel Rice Plant Height Gene

Abstract第7-9页
Abbreviation第10-11页
Chapter Ⅰ第11-34页
    1 Introduction第11-34页
        1.1 Origin and geographical distribution of rice第11-12页
        1.2 Genetic and molecular bases of flowering time第12-14页
        1.3 Rice photoperiod flowering pathways compared to Arabidopsis第14-22页
        1.4 Genetic bases of rice yield traits第22页
        1.5 Genes coordinating rice heading date and yield traits第22-23页
        1.6 Impact of climate changes on rice第23-25页
        1.7 Objectives and Significance第25-26页
        1.8 References第26-34页
CHAPTER Ⅱ第34-57页
    2 Introduction第34-57页
        2.1 Materials and Methods第36-38页
            2.1.1 Plant material and growth chamber conditions for the parents第36页
            2.1.2 Field experimental design第36-37页
            2.1.3 Measurement of heading date and effective cumulative temperature第37页
            2.1.4 DNA isolation and polymorphic marker screening第37-38页
            2.1.5 QTL analysis第38页
            2.1.6 Sequeneing photoperiod genes第38页
        2.2. Results第38-47页
            2.2.1 Heading dates of parents under different photoperiod and temperature conditions第38-40页
            2.2.2 Photoperiod and temperature fluctuations in field experiments第40-41页
            2.2.3 Days to heading第41-42页
            2.2.4 Effective cumulative temperature第42页
            2.2.5 Polymorphism between parents第42-43页
            2.2.6 Location of QTLs第43-44页
            2.2.7 Sequencing analysis of photoperiod-sensitive genes in ZS97 and ZZ18第44-47页
        2.3. Discussion第47-52页
            2.3.1 Growth temperature determines heading date第47页
            2.3.2 HD was controlled by threshold ECTs第47-48页
            2.3.3 Genetic background of the parents is helpful for QTL detection第48-51页
            2.3.4 Roles of ECT in the rice flowering pathway第51-52页
        2.4 References第52-57页
Chapter Ⅲ第57-76页
    3 Introduction第57-76页
        3.1 Materials and Methods第59-61页
            3.1.1 Plant material and field experimental design第59页
            3.1.2 Growth chamber conditions for the parents第59-60页
            3.1.3 Trait measurements第60页
            3.1.4 DNA isolation and polymorphic screening第60页
            3.1.5 Sequencing photoperiod gene第60页
            3.1.6 Data analysis第60-61页
        3.2 Results第61-69页
            3.2.1 Photoperiod and temperature variations among three seasons第61-62页
            3.2.2 Traits response to different seasons第62-64页
            3.2.3 Heritability and correlation第64-65页
            3.2.4 G×E interaction第65页
            3.2.5 Development of genetic linkage maps第65-66页
            3.2.6 QTL analysis by single marker analysis and composite interval mapping第66-68页
            3.2.7 Q×E interactions第68-69页
        3.3 Discussion第69-71页
            3.3.1 Trait performance was mainly influenced by temperature第69-70页
            3.3.2 A major pleiotropic QTL stably expressed in diverse environments第70页
            3.3.3 Importance of environmental effect and G×E in genetic bases of yield-related traits第70-71页
        3.4 References第71-76页
Chapter Ⅳ第76-100页
    4 Introduction第76-100页
        4.1. Materials and Methods第77-80页
            4.1.1 Plant materials and development of mapping population第77-78页
            4.1.2 Fine mapping population第78页
            4.1.3 DNA extraction and marker selection第78页
            4.1.4 Plant height and heading date measurement第78-79页
            4.1.5 Bulk segregant analysis for primary mapping第79页
            4.1.6 Genetic linkage map and genetic effect analysis第79页
            4.1.7 Sequencing analysis of sdl gene and probable candidate gene第79页
            4.1.8 RNA extraction and quantitative real time PCR analysis (qRT-PCR)第79-80页
        4.2. Results第80-92页
            4.2.1 Phenotypic variations of plant height in BC4F2 population and its progeny第80-83页
            4.2.2 Primary mapping of ph1第83-85页
            4.2.3 Fine mapping of ph1第85-88页
            4.2.4 The candidate gene in the 92-kb target region第88-90页
            4.2.5 Co-segregation of the candidate第90-92页
        4.3. Discussion第92-95页
            4.3.1 Comparison of the genetic effects of ph1 to sd1第92-93页
            4.3.2 Chitin-inducible gibberellin-responsive gene might be the probable candidate gene第93-94页
            4.3.3 Plant height gene cluster on chromosome1第94-95页
        4.4. References第95-100页
Resume第100-102页
Acknowledgement第102-103页

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