Abstract | 第7-9页 |
Abbreviation | 第10-11页 |
Chapter Ⅰ | 第11-34页 |
1 Introduction | 第11-34页 |
1.1 Origin and geographical distribution of rice | 第11-12页 |
1.2 Genetic and molecular bases of flowering time | 第12-14页 |
1.3 Rice photoperiod flowering pathways compared to Arabidopsis | 第14-22页 |
1.4 Genetic bases of rice yield traits | 第22页 |
1.5 Genes coordinating rice heading date and yield traits | 第22-23页 |
1.6 Impact of climate changes on rice | 第23-25页 |
1.7 Objectives and Significance | 第25-26页 |
1.8 References | 第26-34页 |
CHAPTER Ⅱ | 第34-57页 |
2 Introduction | 第34-57页 |
2.1 Materials and Methods | 第36-38页 |
2.1.1 Plant material and growth chamber conditions for the parents | 第36页 |
2.1.2 Field experimental design | 第36-37页 |
2.1.3 Measurement of heading date and effective cumulative temperature | 第37页 |
2.1.4 DNA isolation and polymorphic marker screening | 第37-38页 |
2.1.5 QTL analysis | 第38页 |
2.1.6 Sequeneing photoperiod genes | 第38页 |
2.2. Results | 第38-47页 |
2.2.1 Heading dates of parents under different photoperiod and temperature conditions | 第38-40页 |
2.2.2 Photoperiod and temperature fluctuations in field experiments | 第40-41页 |
2.2.3 Days to heading | 第41-42页 |
2.2.4 Effective cumulative temperature | 第42页 |
2.2.5 Polymorphism between parents | 第42-43页 |
2.2.6 Location of QTLs | 第43-44页 |
2.2.7 Sequencing analysis of photoperiod-sensitive genes in ZS97 and ZZ18 | 第44-47页 |
2.3. Discussion | 第47-52页 |
2.3.1 Growth temperature determines heading date | 第47页 |
2.3.2 HD was controlled by threshold ECTs | 第47-48页 |
2.3.3 Genetic background of the parents is helpful for QTL detection | 第48-51页 |
2.3.4 Roles of ECT in the rice flowering pathway | 第51-52页 |
2.4 References | 第52-57页 |
Chapter Ⅲ | 第57-76页 |
3 Introduction | 第57-76页 |
3.1 Materials and Methods | 第59-61页 |
3.1.1 Plant material and field experimental design | 第59页 |
3.1.2 Growth chamber conditions for the parents | 第59-60页 |
3.1.3 Trait measurements | 第60页 |
3.1.4 DNA isolation and polymorphic screening | 第60页 |
3.1.5 Sequencing photoperiod gene | 第60页 |
3.1.6 Data analysis | 第60-61页 |
3.2 Results | 第61-69页 |
3.2.1 Photoperiod and temperature variations among three seasons | 第61-62页 |
3.2.2 Traits response to different seasons | 第62-64页 |
3.2.3 Heritability and correlation | 第64-65页 |
3.2.4 G×E interaction | 第65页 |
3.2.5 Development of genetic linkage maps | 第65-66页 |
3.2.6 QTL analysis by single marker analysis and composite interval mapping | 第66-68页 |
3.2.7 Q×E interactions | 第68-69页 |
3.3 Discussion | 第69-71页 |
3.3.1 Trait performance was mainly influenced by temperature | 第69-70页 |
3.3.2 A major pleiotropic QTL stably expressed in diverse environments | 第70页 |
3.3.3 Importance of environmental effect and G×E in genetic bases of yield-related traits | 第70-71页 |
3.4 References | 第71-76页 |
Chapter Ⅳ | 第76-100页 |
4 Introduction | 第76-100页 |
4.1. Materials and Methods | 第77-80页 |
4.1.1 Plant materials and development of mapping population | 第77-78页 |
4.1.2 Fine mapping population | 第78页 |
4.1.3 DNA extraction and marker selection | 第78页 |
4.1.4 Plant height and heading date measurement | 第78-79页 |
4.1.5 Bulk segregant analysis for primary mapping | 第79页 |
4.1.6 Genetic linkage map and genetic effect analysis | 第79页 |
4.1.7 Sequencing analysis of sdl gene and probable candidate gene | 第79页 |
4.1.8 RNA extraction and quantitative real time PCR analysis (qRT-PCR) | 第79-80页 |
4.2. Results | 第80-92页 |
4.2.1 Phenotypic variations of plant height in BC4F2 population and its progeny | 第80-83页 |
4.2.2 Primary mapping of ph1 | 第83-85页 |
4.2.3 Fine mapping of ph1 | 第85-88页 |
4.2.4 The candidate gene in the 92-kb target region | 第88-90页 |
4.2.5 Co-segregation of the candidate | 第90-92页 |
4.3. Discussion | 第92-95页 |
4.3.1 Comparison of the genetic effects of ph1 to sd1 | 第92-93页 |
4.3.2 Chitin-inducible gibberellin-responsive gene might be the probable candidate gene | 第93-94页 |
4.3.3 Plant height gene cluster on chromosome1 | 第94-95页 |
4.4. References | 第95-100页 |
Resume | 第100-102页 |
Acknowledgement | 第102-103页 |