| DEDICATION | 第9-10页 |
| Acknowledgement | 第10-13页 |
| Contents | 第13-16页 |
| List of Tables | 第16-17页 |
| List of Figures | 第17-21页 |
| List of Abbreviations | 第21-22页 |
| Abstract | 第22-24页 |
| 摘要 | 第25-27页 |
| Chapter 1 Literature review | 第27-53页 |
| 1.1 Tocopherol (Vitamin E) | 第28-31页 |
| 1.2 Plant source of dietary tocopherols | 第31-33页 |
| 1.3 Localization of tocopherols in plant tissue | 第33页 |
| 1.4 Biosynthesis of tocopherols | 第33-34页 |
| 1.5 Enzymes in tocopherols biosynthesis | 第34-35页 |
| 1.6 Tocopherols biosynthesis pathway | 第35-38页 |
| 1.7 Tocopherol and food products | 第38页 |
| 1.8 Tocopherol functions in humans and animals | 第38-41页 |
| 1.9 Tocopherols function as an anti-oxidant | 第41-42页 |
| 1.10 Tocopherols play role in cell signaling and phytohormones modulation | 第42-44页 |
| 1.11 Coordinate response of tocopherols to other antioxidants in abiotic stress tolerance | 第44-47页 |
| 1.12 Functional attributes of tocopherols under water and salt stress | 第47-48页 |
| 1.13 EMS mutation; a reverse genetic approach | 第48-49页 |
| 1.14 SNP Single nucleotide polymorphism | 第49页 |
| 1.15 High resolution melting analysis (HRMA) | 第49-50页 |
| 1.16 Future perspective | 第50-53页 |
| Chapter 2 Detection of tocopherol in oilseed rape(Brassica napus L.)using gaschromatography with flame ionization detector | 第53-73页 |
| 2.1 Materials and methods | 第56-62页 |
| 2.1.1 Seed mateirals | 第56-57页 |
| 2.1.2 Tocopherol extraction protocols | 第57-59页 |
| 2.1.3 Instrumental parameters and temperature programming | 第59页 |
| 2.1.4 Calculations and statistical analysis | 第59-60页 |
| 2.1.5 Standard tocopherol chromatogram and linearity | 第60-61页 |
| 2.1.6 Recovery, limit of detection (LOD) and quantitation (LOQ) | 第61-62页 |
| 2.2 Results | 第62-68页 |
| 2.2.1 Genotypic effect on seed tocopherol | 第62-66页 |
| 2.2.2 Effect of sample preparation protocols on seed tocopherol | 第66页 |
| 2.2.3 Genotypes x protocols interaciton | 第66-68页 |
| 2.3 Discussion | 第68-71页 |
| 2.4 Conclusion | 第71-73页 |
| Chapter 3 Response of seed tocopherols in oilseed rape to Nitrogen fertilizersources and application rates | 第73-102页 |
| 3.1 Material and methods | 第77-81页 |
| 3.1.1 Experimental | 第77-79页 |
| 3.1.2 Sample preparation and Tocopherols analysis | 第79-80页 |
| 3.1.3 GC parameters and temperature programming | 第80页 |
| 3.1.4 GC data computation and statistical analysis | 第80-81页 |
| 3.2 Results | 第81-94页 |
| 3.2.1 Genotypic effect on agronomic traits | 第81-82页 |
| 3.2.2 Effect of N fertilizer sources on agronomic traits | 第82页 |
| 3.2.3 Effect of N application rates on agronomic traits | 第82-85页 |
| 3.2.4 Interactions effects on agronomic traits | 第85-86页 |
| 3.2.5 Response of Toc to N fertilizer sources | 第86-87页 |
| 3.2.6 Response of Toc to N fertilizer application rates | 第87-89页 |
| 3.2.7 Variation in Toc contents of different genotypes of B.Napus L. | 第89-90页 |
| 3.2.8 Response of Toc to the interaction between genotypes and N sources | 第90页 |
| 3.2.9 Response of Toc to the interaction between genotypes and N application rates | 第90-92页 |
| 3.2.10 Response of Toc to the interaction between N sources and N application rates | 第92-94页 |
| 3.2.11 Correlations between Toc isomers,T-Toc,and α-/γ-Toc ratio | 第94页 |
| 3.3 Discussion | 第94-101页 |
| 3.3.1 Effects of genotypes,N sources and N application rates on agronomic traits | 第95-96页 |
| 3.3.2 Interactions effects on agronomic traits | 第96页 |
| 3.3.3 Response of Toc to N sources and N application rates | 第96-98页 |
| 3.3.4 Variation in Toc contents of the five genotypes | 第98-99页 |
| 3.3.5 Interactions effects on seed Toc | 第99-101页 |
| 3.4 Conclusion | 第101-102页 |
| Chapter 4 The association between SN Ps at BnaX.VTE4.blocus and α-/γ-tocopherol ratio in oilseed rape(Brassica napus L.) | 第102-134页 |
| 4.1 Material and methods | 第105-112页 |
| 4.1.1 Reference standards and solvents for tocopherol analysis | 第106页 |
| 4.1.2 Tocopherol extraction from Brassica napus L.seeds | 第106-107页 |
| 4.1.3 Tocopherol analysis using GC-FID coupling | 第107页 |
| 4.1.4 Steps for allelic variation studies | 第107-111页 |
| 4.1.5 Real-Time PCR and HRM analysis | 第111页 |
| 4.1.6 DNA sequencing and polymorphism detection | 第111-112页 |
| 4.1.7 Calculations and statistical analysis | 第112页 |
| 4.2 Results | 第112-131页 |
| 4.2.1 Phenotypic variation of tocopherol contents and composition | 第112-118页 |
| 4.2.2 Real-Time PCR and HRM analysis | 第118-128页 |
| 4.2.3 Linkage disequilibrium and association analysis | 第128-131页 |
| 4.3 Discussion | 第131-133页 |
| 4.4 Conclusions | 第133-134页 |
| References | 第134-158页 |
| Publications | 第158-159页 |
