| 摘要 | 第3-6页 |
| Abstract | 第6-8页 |
| List of abbreviations | 第12-13页 |
| Chapter 1 General introduction | 第13-37页 |
| 1 Social interaction | 第13-14页 |
| 2 The neuroendocrine mechanism of social interaction | 第14-31页 |
| 2.1 The social interaction and AVP systerm | 第14-19页 |
| 2.2 The social interaction and OT systerm | 第19-28页 |
| 2.3 The social interaction and DA systerm | 第28-31页 |
| 3 Early-life social experiences influencing social interaction | 第31-33页 |
| 4 The mandarin vole:an excellent model species for examining the neurobiology of social interaction | 第33-34页 |
| 5 The scope of our study | 第34-37页 |
| Chapter 2 Naturally occurring differences in levels of sociability are associated with the levels of central dopamine,arginine vasopressin,oxytocin and the expression of dopamine (D1 and D2),arginine vasopressin (V1a),oxytocin receptors | 第37-65页 |
| 1 Introduction | 第37-40页 |
| 2 Materials and methods | 第40-45页 |
| 2.1 Subjects | 第40页 |
| 2.2 Social preference tests | 第40-41页 |
| 2.3 Immunocytochemistry for OT, AVP, TH | 第41-42页 |
| 2.4 The expression of OTR, AV PV1aR, D1R and D2R in the NAcc and MeA | 第42-44页 |
| 2.4.1 NAcc and MeA tissue collection | 第42页 |
| 2.4.2 RNA extraction | 第42-43页 |
| 2.4.3 RT PCR analysis | 第43页 |
| 2.4.4 Western blot analysis | 第43-44页 |
| 2.5 Statistical analysis | 第44-45页 |
| 3 Results | 第45-60页 |
| 3.1 Social preference tests | 第45-46页 |
| 3.2 Immunocytochemistry for OT, AVP, TH | 第46-50页 |
| 3.3 The expression of OTR, AVPV1aR, D1R and D2R in the NAcc and MeA | 第50-60页 |
| 3.3.1 RT PCR analysis | 第50-55页 |
| 3.3.2 Western blot analysis | 第55-60页 |
| 4 Discussion | 第60-65页 |
| 4.1 Different levels of OT system parameters in mandarin voles with different levels of sociability | 第60-62页 |
| 4.2 Different levels of AVP system parameters in mandarin voles with different levels of sociability | 第62-63页 |
| 4.3 Different levels of sociability with DA system in mandarin voles | 第63-65页 |
| Chapter 3 Naturally occurring differences in parental care are associated with the levels of sociability and central levels of dopamine, arginine vasopressin, oxytocin and the expression of dopamine (D1 and D2),arginine vasopressin (V1a), oxytocin receptor | 第65-85页 |
| 1 Introduction | 第65-67页 |
| 2 Materials and methods | 第67-69页 |
| 2.1 Subjects | 第67页 |
| 2.2 parental behavior observation | 第67-68页 |
| 2.3 Social preference tests | 第68页 |
| 2.4 Immunocytochemistry for OT, AVP, TH | 第68页 |
| 2.5 The expression of OTR, AVPV1aR, D1R and D2R in the NAcc and MeA | 第68-69页 |
| 2.6 Statistical analysis | 第69页 |
| 3 Results | 第69-80页 |
| 3.1 parental behavior observation | 第69页 |
| 3.2 Social preference tests | 第69-70页 |
| 3.3 Immunocytochemistry for OT, AVP, TH | 第70-75页 |
| 3.4 The expression of OTR, AVPV1aR, DIR and D2R in the NAcc and MeA | 第75-80页 |
| 4 Disscussion | 第80-85页 |
| Chapter 4 Effects of nucleus accumbens oxytocin and its antagonist on social approach behavior | 第85-101页 |
| 1 Introduction | 第85-87页 |
| 2 Materials and methods | 第87-90页 |
| 2.1 Subjects | 第87页 |
| 2.2 Social preference tests | 第87页 |
| 2.3 Open field test | 第87-88页 |
| 2.4 Stereotaxic cannulation and microinjection | 第88-89页 |
| 2.5 Experimental design | 第89-90页 |
| 2.5.1 Selection of animals with different levels of sociability | 第89页 |
| 2.5.2 OT and OTR-A treatments | 第89-90页 |
| 2.6 Statistical analyses | 第90页 |
| 3 Results | 第90-95页 |
| 3.1 Approach and avoidance behavior | 第90-92页 |
| 3.2 Effect of OTR-A treatment in mandarin voles with high sociability | 第92-94页 |
| 3.3 Effects of OT treatment in mandarin voles with lower sociability | 第94-95页 |
| 4 Discussion | 第95-101页 |
| Chapter 5 Summary and future directions | 第101-105页 |
| References | 第105-143页 |
| Acknowledgements | 第143-145页 |
| List of publications | 第145页 |
