| 摘要 | 第6-7页 |
| Abstract | 第7页 |
| Abbreviations | 第15-17页 |
| CHAPTER1 INTRODUCTION | 第17-26页 |
| 1.1 Background of migratory locust | 第17-18页 |
| 1.2 Damage behavior of migratory locusts | 第18页 |
| 1.3 Chemical control and their disadvantages | 第18-19页 |
| 1.4 Biological control | 第19页 |
| 1.5 Genetic and hormonal control | 第19-24页 |
| 1.5.1 Insect diapause | 第19-20页 |
| 1.5.2 Maternal photoperiod effect on diapause | 第20页 |
| 1.5.3 Role of retinoids in insect diapause | 第20-21页 |
| 1.5.4 Role of circadian clock genes during insect diapause | 第21-23页 |
| 1.5.5 Role of Insulin signaling/FOXO pathways in diapause induction | 第23页 |
| 1.5.6 Role of insulin receptors | 第23-24页 |
| 1.5.7 ROS activate the insulin signaling/FOXO | 第24页 |
| 1.6 Molecular technique’s used in the present study | 第24-25页 |
| 1.7 Objectives | 第25页 |
| 1.8 Techanical route of the present study | 第25-26页 |
| Chapter2 Transcriptomic analysis from fat body and ovary of L.migratoria | 第26-41页 |
| 2.1 Summary | 第26页 |
| 2.2 Materials and Methods | 第26-31页 |
| 2.2.1 Ethics Statement | 第26页 |
| 2.2.2 Insect rearing | 第26-27页 |
| 2.2.3 Dissection and sample collection method | 第27页 |
| 2.2.4 RNA extraction | 第27-28页 |
| 2.2.5 c DNA and RNA sequencing | 第28-29页 |
| 2.2.6 Sequence assembly,annotation and DEGs analysis | 第29页 |
| 2.2.7 Gene expressions by qRT-PCR | 第29-30页 |
| 2.2.8 Statistical analysis | 第30-31页 |
| 2.3 Results | 第31-39页 |
| 2.3.1 Transcriptomic analysis | 第31-32页 |
| 2.3.2 Differentially expressed genes(DEGs)between long photoperiod and short photoperiods in L.migratoria | 第32-33页 |
| 2.3.3 Correlation analysis of Differentally Expressed Genes(DEGs)amongst long and short photoperiods | 第33-34页 |
| 2.3.4 Different Expressed Genes(DEGs)functions in Cluster wise series | 第34-35页 |
| 2.3.5 KEGG pathways classification | 第35-37页 |
| 2.3.6 Validations of DEGs by quantitative real-time PCR(qRT-PCR) | 第37-39页 |
| 2.4 Discussion | 第39-41页 |
| Chapter3 Molecular cloning of rai1,shkC and their effect on maternal offspring diapause of L.migratoria | 第41-51页 |
| 3.1 Summary | 第41页 |
| 3.2 Material and Methods | 第41-46页 |
| 3.2.1 Insect rearing and sample collection method | 第41页 |
| 3.2.2 RNA Extraction | 第41-42页 |
| 3.2.3 cDNA Synthesis | 第42页 |
| 3.2.4 Molecular cloning and sequencing of rai1 and shkC | 第42-43页 |
| 3.2.5 dsRNA synthesis | 第43-44页 |
| 3.2.6 RNA interference by qRT-PCR | 第44页 |
| 3.2.7 Egg collection and diapause detection | 第44-45页 |
| 3.2.8 SOD and ROS activity detection | 第45页 |
| 3.2.9 Statistical analysis | 第45-46页 |
| 3.3 Results | 第46-49页 |
| 3.3.1 Cloning and Sequencing of rai1 and shkC | 第46页 |
| 3.3.2 RNAi efficiency and maternal diapause | 第46-47页 |
| 3.3.3 SOD and ROS activity changes after RNAi rai | 第47-49页 |
| 3.4 Discussion | 第49-51页 |
| Chapter4 Transcriptomic analysis from CNS of L.migratoria | 第51-59页 |
| 4.1 Summary | 第51页 |
| 4.2 Material and Methods | 第51-52页 |
| 4.2.1 Insect rearing | 第51页 |
| 4.2.2 Dissection and sample collection method | 第51页 |
| 4.2.3 RNA extraction and RNA-Seq | 第51页 |
| 4.2.4 Sequence assembly,annotation and DEGs analysis | 第51-52页 |
| 4.2.5 cDNA synthesis and qRT-PCR | 第52页 |
| 4.3 Results | 第52-57页 |
| 4.3.1 Transcriptomes sequencing and annotation assembly | 第52-53页 |
| 4.3.2 Different expressed genes(DEGs)from CNS of L.migratoria | 第53-54页 |
| 4.3.3 Functional classification by GO enrichment of DEGs | 第54-55页 |
| 4.3.4 KEGG pathway classification | 第55-56页 |
| 4.3.5 Gene expressions by using qRT-PCR | 第56-57页 |
| 4.4 Discussion | 第57-59页 |
| Chapter5 Molecular cloning of takeout gene and their effect on maternal offspring diapause of L.migratoria | 第59-64页 |
| 5.1 Summary | 第59页 |
| 5.2 Material and Methods | 第59-61页 |
| 5.2.1 Insect colony rearing and tissues collection | 第59页 |
| 5.2.2 RNA extraction | 第59页 |
| 5.2.3 cDNA synthesis | 第59-60页 |
| 5.2.4 Molecular cloning and sequencing of takeout gene | 第60页 |
| 5.2.5 dsRNA synthesis | 第60页 |
| 5.2.6 RNA interference | 第60页 |
| 5.2.7 Egg collection and diapause detection | 第60-61页 |
| 5.3 Results | 第61-62页 |
| 5.3.1 Cloning and sequencing of takeout gene | 第61页 |
| 5.3.2 RNA interference of dstakeout and maternal diapause | 第61-62页 |
| 5.4 Discussion | 第62-64页 |
| Chapter6 Conclusion | 第64-66页 |
| References | 第66-73页 |
| Appendix | 第73-76页 |
| Acknowledgement | 第76-77页 |
| Author Biography | 第77-81页 |
