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玉米须活性成分的提取分离及其生物活性研究

中文摘要第3-6页
ABSTRACT第6-7页
Chapter1 General introduction第12-24页
    1.1 Research progress of corn silk第12-19页
        1.1.1 Chemical components of corn silk第12-14页
        1.1.2 Pharmacological activity of corn silk第14-19页
    1.2 Introduction to metal nanoparticles第19-23页
        1.2.1 Synthesis of nanoparticles using plant extracts第20-21页
        1.2.2 Characterization of biosynthesized nanoparticles第21-22页
        1.2.3 Applications of biosynthesized nanoparticles第22-23页
    1.3 The purpose and significance of the study第23-24页
Chapter2 Extraction and purification of corn silk polysaccharides and their bioactivities第24-40页
    2.1 Introduction第24页
    2.2 Materials第24-25页
        2.2.1 Materials and chemicals第24-25页
        2.2.2 Animals第25页
    2.3 Methods第25-28页
        2.3.1 Isolation of polysaccharides第25页
        2.3.2 Determination of antioxidant and α-amylase inhibitory activity of PCS1,PCS2 and PCS3第25-26页
        2.3.3 Structural characterization of PCS2第26页
        2.3.4 Animal experimental design第26-27页
        2.3.5 Estimation of fasting blood glucose(FBG)第27页
        2.3.6 Oral glucose tolerance test(OGTT)第27页
        2.3.7 Biochemical analysis第27-28页
        2.3.8 Histological studies第28页
        2.3.9 Statistical analyses第28页
    2.4 Results and discussion第28-38页
        2.4.1 Isolation of polysaccharides第28-29页
        2.4.2 Antioxidant activity and α-amylase inhibitory activity of PCS1,PCS2 and PCS3第29页
        2.4.3 Structural analysis of PCS2第29-31页
        2.4.4 Food intake,water consumption and body weight of mice第31-32页
        2.4.5 Effect of PCS2 on FBG levels and oral glucose tolerance in diabetic mice第32-34页
        2.4.6 Effect of PCS2 on serum insulin levels in diabetic mice第34页
        2.4.7 Effect of PCS2 on GSP,NEFA and lipid profile levels in diabetic mice第34-36页
        2.4.8 Effect of PCS2 on glycogen levels第36页
        2.4.9 Effect of PCS2 on the activities of CAT,GPX,SOD and level of MDA in diabetic mice第36-37页
        2.4.10 Histopathological observation第37-38页
    2.5 Conclusion第38-40页
Chapter3 Extraction,separation and bioactivities of small molecules in corn silk第40-62页
    3.1 Introduction第40页
    3.2 Materials第40-41页
    3.3 Methods第41-45页
        3.3.1 Extraction process and quantification of total polyphenol,total flavonoids,and total sterols contents第41页
        3.3.2 Determination of antioxidant, α-amylase andα-glucosidase inhibitory activities of PEF,EAF and NBF第41-42页
        3.3.3 LC-MS/MS analysis of NBF第42页
        3.3.4 Animal experimental design第42-43页
        3.3.5 Estimation of fasting blood glucose(FBG)第43页
        3.3.6 Oral glucose tolerance test(OGTT)第43页
        3.3.7 Biochemical analysis第43-44页
        3.3.8 Histological studies第44页
        3.3.9 Separation of NBF第44页
        3.3.10 Statistical analyses第44-45页
    3.4 Results and discussion第45-61页
        3.4.1 The analysis of total phenolics,flavonoids and sterols第45-46页
        3.4.2 Antioxidant activity, α-amylase inhibitory activity andα-glucosidase inhibitory activity of different fractions第46-47页
        3.4.3 LC-MS/MS analysis of NBF第47-49页
        3.4.4 Animal experiments第49-57页
        3.4.5 Purification and identification of the constitunents of NBF第57-61页
    3.5 Conclusion第61-62页
Chapter4 Green synthesis of silver nanoparticles using corn silk extract and their bioactivities第62-74页
    4.1 Introduction第62页
    4.2 Materials第62页
    4.3 Methods第62-66页
        4.3.1 Sample Preparation第62-63页
        4.3.2 Total phenolic assay of CSAE第63页
        4.3.3 Total flavonoids assay of CSAE第63页
        4.3.4 Total reducing sugars estimation of CSAE第63页
        4.3.5 Synthesis of silver nanoparticles using CSAE第63-64页
        4.3.6 Characterization of synthesized nanoparticles第64-65页
        4.3.7 Evaluating the biological activities of AgNPs第65-66页
        4.3.8 Statistical Analysis第66页
    4.4 Results and discussion第66-72页
    4.5 Conclusion第72-74页
Chapter5 Conclusions and prospects第74-76页
References第76-86页
The list of abbreviations第86-88页
General Experimental Procedures第88-94页
Achievements第94-96页
Acknowledgements第96页

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