| Abstract in Chinese | 第8-10页 |
| Abstract in English | 第10-11页 |
| 1 Introduction | 第12-20页 |
| 1.1 Aflatoxins | 第12-13页 |
| 1.1.1 Description of aflatoxins | 第12页 |
| 1.1.2 Types of Aflatoxin | 第12页 |
| 1.1.3 Overview of aflatoxin B1 | 第12-13页 |
| 1.1.4 Metabolism and bioactivation of AFB1 | 第13页 |
| 1.2 Liver phase-I (cytochrome p450) enzymes | 第13-15页 |
| 1.2.1 Introduction to cytochrome p450 enzymes | 第13-14页 |
| 1.2.2 Nomenclature of CYP450 enzymes | 第14页 |
| 1.2.3 Role of CYP450 enzymes in xenobiotics and drug metabolism | 第14页 |
| 1.2.4 Applications of CYP450 | 第14-15页 |
| 1.3 Illustration of CYP2A family | 第15-16页 |
| 1.3.1 CYP2A subfamily and CYP2A6 enzyme | 第15页 |
| 1.3.2 CYP2A6 expression and its role in hepatic injury | 第15页 |
| 1.3.3 Current perspectives of CYP2A6 | 第15-16页 |
| 1.4 Curcumin | 第16-18页 |
| 1.4.1 Introduction to curcumin | 第16-17页 |
| 1.4.2 Preventive and therapeutic uses of curcumin | 第17页 |
| 1.4.3 Effect of curcumin on cytochrome p450 enzymes | 第17-18页 |
| 1.4.4 Curcumin as an effective drug against AFB1 toxicity | 第18页 |
| 1.5 Purpose and significance of the study | 第18-20页 |
| 2 Material and methods | 第20-26页 |
| 2.1 Material | 第20-21页 |
| 2.1.1 Drugs and Chemical reagents | 第20页 |
| 2.1.2 Kits and Apparatus | 第20页 |
| 2.1.3 Arbor Acres broilers | 第20-21页 |
| 2.1.4 Preparation of the main solutions used in experiments | 第21页 |
| 2.2 Methods | 第21-26页 |
| 2.2.1 Animal experiment | 第21-22页 |
| 2.2.2 Histopathological examination | 第22-23页 |
| 2.2.3 CYP2A6 gene primer designing and polymerase chain reaction | 第23-24页 |
| 2.2.4 Extraction and isolation of Ribonucleic acid (RNA) and quantitative real time qPCR | 第24页 |
| 2.2.5 Determination of CYP2A6 enzyme activity | 第24-25页 |
| 2.2.6 Enzyme linked immunosorbant assay (ELISA) for CYP2A6 enzyme | 第25页 |
| 2.2.7 Statistical analysis | 第25-26页 |
| 3 Results | 第26-39页 |
| 3.1 Effects of AFB1 on liver weight of broilers | 第26-27页 |
| 3.2 Effects of AFB1 on body weight of broilers | 第27-28页 |
| 3.3 Liver histopathology | 第28-29页 |
| 3.4 Polymerase chain reaction (PCR) of CYP2A6 gene, sequencing and construction of phylogenetic tree | 第29-32页 |
| 3.5 Effect of curcumin on AFB1-induced CYP2A6 mRNA expression | 第32-33页 |
| 3.6 Effect of curcumin on AFB1-induced CYP2A6 enzyme activity | 第33-34页 |
| 3.7 Effect of curcumin on AFB1-induced CYP2A6 protein expression level | 第34-35页 |
| 3.8 Effect of curcumin on AFB1-induced CYP1A1 mRNA expression | 第35-36页 |
| 3.9 Effect of curcumin on AFB1-induced CYP1A2 mRNA expression | 第36-37页 |
| 3.10 Effect of curcumin on AFB1-induced CYP3A4 mRNA expression | 第37-39页 |
| 4 Discussion | 第39-43页 |
| 4.1 Effect of AFB1 on body weight and liver weight of Arbor Acres broiler | 第39页 |
| 4.2 AFB1 induced liver injury | 第39-40页 |
| 4.3 Role of CYP2A6 enzyme in bioactivation of AFB1 | 第40页 |
| 4.4 Curcumin successfully inhibited cytochrome p450 enzymes-mediated bioactivation of AFB1 in AA broiler liver | 第40-43页 |
| 5 Conclusion | 第43-44页 |
| Acknowledgement | 第44-45页 |
| References | 第45-53页 |
| Papers published in the period of Ph.M. education | 第53-55页 |
