| Abstract | 第6-7页 |
| 摘要 | 第8-14页 |
| Chapter Ⅰ: Introduction | 第14-24页 |
| 1.1 Overview of CBL family | 第14页 |
| 1.2 Structure and mechanism of CBL | 第14-16页 |
| 1.3 Functions of CBL in plant | 第16-20页 |
| 1.3.1 CBL is involved in response to biotic and abiotic stress | 第16-18页 |
| 1.3.2 Transgenic CBL plant has great role in crop production | 第18-20页 |
| 1.4 Prospective research of CBL genes | 第20-23页 |
| 1.5 Research aims and objectives | 第23-24页 |
| Chapter Ⅱ: Materials and Methods | 第24-39页 |
| 2.1 Materials | 第24-28页 |
| 2.1.1 Plant Materials | 第24页 |
| 2.1.2 Chemical materials | 第24-26页 |
| 2.1.3 Competent cells and vectors | 第26页 |
| 2.1.4 Primers | 第26-28页 |
| 2.1.5 Softwares and databases | 第28页 |
| 2.2 Methods of Experiments | 第28-39页 |
| 2.2.1 Bioinformatics and softwares analysis | 第28-29页 |
| 2.2.2 Construction methods of vectors | 第29-30页 |
| 2.2.3 Protocols of extractions, measurement and PCR | 第30-35页 |
| 2.2.4 Transformation techniques of bacteria | 第35-38页 |
| 2.2.5 Methods of stress treatments of Arabidopsis | 第38-39页 |
| Chapter Ⅲ: Results | 第39-60页 |
| 3.1 Isolation and phylogenetic results of Nsyl CBL2 | 第39-41页 |
| 3.2 Structure, physicochemical and expression level analysis of Nsyl CBL2 | 第41-43页 |
| 3.3 Functional analysis of Nsyl CBL2 in Arabidopsis | 第43-53页 |
| 3.3.1 Identification and selection results of over-expressed Nsyl CBL2 lines | 第43-46页 |
| 3.3.2 Morphological analysis of Nsyl CBL2 over expressing plants | 第46-47页 |
| 3.3.3 Results of atcbl2 mutant analysis | 第47-48页 |
| 3.3.4 Results analysis of Nsyl CBL2-OE plants under abiotic stress conditions | 第48-53页 |
| 3.4 Characterization of Nsyl CBL2 in Tobacco | 第53-58页 |
| 3.4.1 Acquire transgenic Nsyl CBL2 tobacco plant | 第53-55页 |
| 3.4.2 Over -expression level analysis of Nsyl CBL2 in tobacco | 第55-56页 |
| 3.4.3 Obtain Nsyl CBL2 over expression T2 tobacco lines by resistant screening test | 第56-58页 |
| 3.5 Promoter analysis of Nsyl CBL2 | 第58-60页 |
| Chapter Ⅳ: Discussion | 第60-63页 |
| 4.1 Bioinformatics analysis revealed that Nsyl CBL2 has common mode of action in signalling | 第60-61页 |
| 4.2 Nsyl CBL2 responds differently than Arabidopsis CBL2 in particular stress conditions | 第61-63页 |
| Chapter Ⅴ: Conclusion | 第63-64页 |
| References | 第64-69页 |
| Acknowledgements | 第69-70页 |
| Author Biography | 第70页 |
