| Abstract | 第10-12页 |
| Abstract in Chinese | 第13-15页 |
| Chapter.1 Review Research Progress on Tobacco Target Spot Disease and Biocontrol of PlantDisease | 第15-30页 |
| Chapter.2 Isolation and Screening of Antagonistic Microorganism against the Rhizoctonia solani AG-3 | 第30-38页 |
| 2.1 Materials and Methods | 第31-33页 |
| 2.2 Results | 第33-36页 |
| 2.2.1 Primary Screening | 第33-34页 |
| 2.2.2 Secondary Screening | 第34-35页 |
| 2.2.3 Antimicrobial Effects of Antagonistic Streptomyces against Test Pathogens | 第35-36页 |
| 2.3 Brief Summary | 第36-38页 |
| Chapter.3 Identification and Taxonomy of Streptomyces TA1123 | 第38-49页 |
| 3.1 Materials and Methods | 第39-41页 |
| 3.2 Results and Analysis | 第41-47页 |
| 3.2.1 Morphological Analysis | 第41-43页 |
| 3.2.2 Chemotaxonomy | 第43页 |
| 3.2.3 Physiology Test | 第43-46页 |
| 3.2.4 Phylogentic Analysis | 第46-47页 |
| 3.3 Brief Summary | 第47-49页 |
| Chapter.4 Fermentation conditions of Streptomyces TA1123 in Shaking Flask | 第49-60页 |
| 4.1 Materials and Methods | 第50-53页 |
| 4.2 Results and Analysis | 第53-58页 |
| 4.2.1 Optimization of Fermentation Conditions | 第53-58页 |
| 4.3 Brief Summary | 第58-60页 |
| Chapter.5 Optimization of medium for the Production of Secondary Metabolites by Streptomyces strain | 第60-74页 |
| 5.1 Material and Methods | 第60-64页 |
| 5.2 Results and Analysis | 第64-71页 |
| 5.2.1 Effect of Carbon，Nitrogen and Microelemeiit Sources on Antifungal Activity of Strain TA1123 | 第64-66页 |
| 5.2.2 Optimization of Fermentation Medium through CCD | 第66-71页 |
| 5.2.3 Metabolism of Streptomyces Strain TAl123 during Fermentation in Shaking Flasks | 第71页 |
| 5.3 Berief Summary | 第71-74页 |
| Chapter.6 Extraction, Purification and Identification of Secondary Metabolites | 第74-88页 |
| 6.1 Material and Methods | 第75-76页 |
| 6.2 Results and Analysis | 第76-85页 |
| 6.2.1 Stability of the Fermentation Broth | 第77-78页 |
| 6.2.2 Solvent Extraction of the Fermentation Broth | 第78-79页 |
| 6.2.3 Purification of Extracted Crude by Silica gel column chromatography | 第79-80页 |
| 6.2.4 Compound Identification in Ethyl acetate Extract of Streptomyces TA112 by GC-MS | 第80-83页 |
| 6.2.5 Compound Identification in Diethyl ether Extract | 第83-85页 |
| 6.3. Brief Summary | 第85-88页 |
| Chapter.7 Action Mechanism of Ethyl Acetate Extract of Streptomyces TA1123 against the Rhizoctonia solani AG-3 | 第88-95页 |
| 7.1 Materials and Methods | 第89-90页 |
| 7.2 Results and Analysis | 第90-93页 |
| 7.2.1 Suppression of Mycelial Growth of R.solani AG-3 | 第90-91页 |
| 7.2.2 Effect of Ethyl Acetate Extract of the Streptomyces TA1123 on the Formation of Sclerotia | 第91页 |
| 7.2.3 Observation of the Hyphae | 第91-92页 |
| 7.2.4 Determination of Endogenous ROS | 第92-93页 |
| 7.2.5 Inhibition Effects of the Ethyl Acetate Extract of Streptomyces TA1123 Against the Lesion of the Disease | 第93页 |
| 7.3 Brief Summary | 第93-95页 |
| Chapter.8 Effects of Ethyl Acetate Extracts of Streptomyces TA1123 on Defense Enzyme and Extracellular Metablism in Tobacco Leave | 第95-111页 |
| 8.1 Materials and Methods | 第96-98页 |
| 8.2 Results and Analysis | 第98-108页 |
| 8.2.1 Activity of Glutathione-S- Transferase(GST) in Tobacco leaves of Different Treatments | 第98-99页 |
| 8.2.2 Activity of Glutathione Redudase(GR) in Tobacco Leaves of Different Treatments | 第99页 |
| 8.2.3 Activities of Glutathione Peroxide(GPX) in Tobacco Leaves of Different Treatments | 第99-100页 |
| 8.2.4 Activity of Peroxidase(POD) in Tobacco Leaves of Different Treatments | 第100-101页 |
| 8.2.5 Activity of Catalase(CAT) in Tobacco Leaves of Different Treatments | 第101-102页 |
| 8.2.6 Activity of Superoxide disniutase(SOD) in Tobacco Leaves of Different Treatments | 第102-103页 |
| 8.2.7 Activity of Phenylalanine Ammonia-Lyase(PAL) in Tobacco Leaves of Different Teatments | 第103页 |
| 8.2.8 Activity of Polyphenol Oxidase(PPO) in Tobacco Leaves of | 第103-104页 |
| 8.2.9 Analysis of Metabolites Changes in Tobacco Plant Induced by the Ethyl Acetate Extract of Streptomyces TA1123 | 第104-108页 |
| 8.3 Brief Summary | 第108-111页 |
| Conclusion | 第111-114页 |
| References | 第114-129页 |
| Acknowledgements | 第129-130页 |
| Publications during Study | 第130页 |
