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BIGLYCAN在光老化中的作用机制研究

中文摘要第6-10页
ABSTRACT第10-14页
ABBREVIATIONS第17-18页
PART ONE第18-52页
    1. Introduction第19-21页
    2. Materials and methods第21-26页
        2.1 Cell culture and UV irradiation第21页
        2.2 Quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR)第21-22页
        2.3 Gene silencing with siRNA第22-23页
        2.4 Overexpression of wild-type and mutant BGN proteins in primary human dermalfibroblasts第23-24页
        2.5 Western blot analysis第24-25页
        2.6 Statistics第25-26页
    3. Results第26-45页
        3.1 UV irradiation increased defectively-glycosylated BGN expression dose-dependently incultured human dermal fibroblasts第26-32页
        3.2 UV-induced mRNA expression changes of xylosyltransferases(XYLTs) in cultured humandermal fibroblasts第32-34页
        3.3 Downregulation of XYLT1 by siRNA transfection impairs BGN GAG synthesis incultured human dermal fibroblasts第34-38页
        3.4 Another DS proteoglycan,DCN,showed a little or no decrease of its molecular weight byUV irradiation and XYLT1 siRNA transfection in cultured human dermal fibroblasts第38-40页
        3.5 XYLT1 can add xylose on S42 and S47 on the core protein in cultured human dermalfibroblasts,while XYLT2 can add xylose only on S42,but not S47第40-45页
    4. Discussion第45-51页
    5. Conclusions第51-52页
PART TWO第52-67页
    1. Introduction第53-55页
    2. Materials and Methods第55-58页
        2.1 Cell culture and UV irradiation第55页
        2.2 UV and LPS stimulation第55页
        2.3 Quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR)第55-56页
        2.4 Gene silencing with siRNA第56-57页
        2.5 Statistics第57-58页
    3. Results第58-64页
        3.1 Down-Regulation of BGN prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts第58-60页
        3.2 Down-Regulation of TLR4 prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts第60-62页
        3.3 Down-Regulation of BGN and TLR4 prevents LPS-induced IL-6,IL-8 gene expression inhuman fibroblasts第62-64页
    4. Discussion第64-66页
    5. Conclusions第66-67页
References第67-72页
综述第72-89页
    参考文献第82-89页
致谢第89页

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