| 中文摘要 | 第6-10页 |
| ABSTRACT | 第10-14页 |
| ABBREVIATIONS | 第17-18页 |
| PART ONE | 第18-52页 |
| 1. Introduction | 第19-21页 |
| 2. Materials and methods | 第21-26页 |
| 2.1 Cell culture and UV irradiation | 第21页 |
| 2.2 Quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR) | 第21-22页 |
| 2.3 Gene silencing with siRNA | 第22-23页 |
| 2.4 Overexpression of wild-type and mutant BGN proteins in primary human dermalfibroblasts | 第23-24页 |
| 2.5 Western blot analysis | 第24-25页 |
| 2.6 Statistics | 第25-26页 |
| 3. Results | 第26-45页 |
| 3.1 UV irradiation increased defectively-glycosylated BGN expression dose-dependently incultured human dermal fibroblasts | 第26-32页 |
| 3.2 UV-induced mRNA expression changes of xylosyltransferases(XYLTs) in cultured humandermal fibroblasts | 第32-34页 |
| 3.3 Downregulation of XYLT1 by siRNA transfection impairs BGN GAG synthesis incultured human dermal fibroblasts | 第34-38页 |
| 3.4 Another DS proteoglycan,DCN,showed a little or no decrease of its molecular weight byUV irradiation and XYLT1 siRNA transfection in cultured human dermal fibroblasts | 第38-40页 |
| 3.5 XYLT1 can add xylose on S42 and S47 on the core protein in cultured human dermalfibroblasts,while XYLT2 can add xylose only on S42,but not S47 | 第40-45页 |
| 4. Discussion | 第45-51页 |
| 5. Conclusions | 第51-52页 |
| PART TWO | 第52-67页 |
| 1. Introduction | 第53-55页 |
| 2. Materials and Methods | 第55-58页 |
| 2.1 Cell culture and UV irradiation | 第55页 |
| 2.2 UV and LPS stimulation | 第55页 |
| 2.3 Quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR) | 第55-56页 |
| 2.4 Gene silencing with siRNA | 第56-57页 |
| 2.5 Statistics | 第57-58页 |
| 3. Results | 第58-64页 |
| 3.1 Down-Regulation of BGN prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts | 第58-60页 |
| 3.2 Down-Regulation of TLR4 prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts | 第60-62页 |
| 3.3 Down-Regulation of BGN and TLR4 prevents LPS-induced IL-6,IL-8 gene expression inhuman fibroblasts | 第62-64页 |
| 4. Discussion | 第64-66页 |
| 5. Conclusions | 第66-67页 |
| References | 第67-72页 |
| 综述 | 第72-89页 |
| 参考文献 | 第82-89页 |
| 致谢 | 第89页 |
