| 摘要 | 第6-8页 |
| Abstract | 第8-9页 |
| CHAPTER 1 INTRODUCTION | 第13-42页 |
| 1.1 CORONARY ARTERY DISEASE THERAPY | 第13-23页 |
| 1.1.1 Cardiovascular disease, atherosclerosis | 第13-15页 |
| 1.1.2 Coronary artery bypass graft surgery and vascular grafts | 第15-17页 |
| 1.1.3 Percutaneous coronary intervention and coronary artery stents | 第17-23页 |
| 1.2 ENDOTHELIUM FUNCTION MIMICKING SURFACE MODIFICATION | 第23-32页 |
| 1.2.1 ECs functions | 第23-26页 |
| 1.2.2 Heparin based surface modification | 第26-29页 |
| 1.2.3 Nitric oxide based surface modification | 第29-32页 |
| 1.3 PROGRESS OF POLYDOPAMINE COATING IN BIOMEDICAL FIELD | 第32-38页 |
| 1.3.1 Coating mechanism, structure and preparation | 第32-36页 |
| 1.3.2 Applications in biomedical field | 第36-38页 |
| 1.4 SIGNIFICANCE AND OBJECTIVES,DISSERTATION LAYOUT AND TECHNICAL ROUTE | 第38-42页 |
| 1.4.1 Significance and objectives | 第38-39页 |
| 1.4.2 Dissertation layout | 第39-41页 |
| 1.4.3 Technical route | 第41-42页 |
| CHAPTER 2 MUSSEL-INSPIRED CHEMISTRY FOR BIOCOMPATIBLE ANDFUNCTIONAL COATINGS RICH IN AMINE GROUPS | 第42-69页 |
| 2.1 INTRODUCTION | 第42-43页 |
| 2.2 EXPERIMENTAL SECTION | 第43-47页 |
| 2.2.1 Preparation of the PDAM/HD copolymer coating | 第43页 |
| 2.2.2 Characterization of the PDAM/HD copolymer coating | 第43-45页 |
| 2.2.3 HUVEC culture,staining and proliferation | 第45页 |
| 2.2.4 Macrophages evaluation and in vivo tissue response test | 第45-46页 |
| 2.2.5 Heparin conjugation and quantitative determination | 第46-47页 |
| 2.2.6 APTT assay | 第47页 |
| 2.2.7 Statistical analysis | 第47页 |
| 2.3 RESULTS ANALYSIS | 第47-63页 |
| 2.3.1 FTIR,amine group density and thickness of PDAM/HD coating | 第47-49页 |
| 2.3.2 XPS,SIMS results | 第49-54页 |
| 2.3.3 AFM and WCA results | 第54-56页 |
| 2.3.4 Mechanical behavior and stability | 第56-57页 |
| 2.3.5 Adhesion,proliferation and staining of endothelium cell | 第57-59页 |
| 2.3.6 Macrophages evaluation and tissue response | 第59-62页 |
| 2.3.7 Conjugation of heparin and bioactivity assay | 第62-63页 |
| 2.4 DISCUSSION | 第63-68页 |
| 2.5 CONCLUSION | 第68-69页 |
| CHAPTER 3 HEPARINIZED MUSSEL-INSPIRED COATING BARING AMINE GROUP FOR MULTI-FUNCTIONALIZATION OF VASCULAR STENT | 第69-95页 |
| 3.1 INTRODUCTION | 第69-71页 |
| 3.2 EXPERIMENTAL SECTION | 第71-76页 |
| 3.2.1 Preparation of PDAM/HD coating | 第71页 |
| 3.2.2 Characterization of PDAM/HD coating | 第71页 |
| 3.2.3 Heparin conjugation and quantification | 第71-72页 |
| 3.2.4 Hemocompatibility evaluation | 第72-73页 |
| 3.2.5 Endothelium cell evaluation | 第73-74页 |
| 3.2.6 Smooth muscle cell evaluation | 第74-75页 |
| 3.2.7 Competitive Adhesion of ECs and SMCs | 第75页 |
| 3.2.8 In vivo Subcutaneous Implantation Studies | 第75页 |
| 3.2.9 Statistical analysis | 第75-76页 |
| 3.3 RESULTS ANALYSIS | 第76-91页 |
| 3.3.1 Characterization of Hep-PDAM/HD | 第76-78页 |
| 3.3.2 Hemocompatibility evalution | 第78-82页 |
| 3.3.3 Endothelial cell evalution | 第82-86页 |
| 3.3.4 Smooth muscle cell evalution | 第86-87页 |
| 3.3.5 Competitive Adhesion of ECs and SMCs | 第87-89页 |
| 3.3.6 In vivo Subcutaneous Implantation Studies | 第89-91页 |
| 3.4 DISCUSSION | 第91-94页 |
| 3.5 CONCLUSION | 第94-95页 |
| CHAPTER 4 BIOMIMETIC DESIGN OF NO-GENERATING COATINGS FORIMPROVING THE PERFORMANCE OF VASCULAR STENT | 第95-122页 |
| 4.1 INTRODUCTION | 第95-98页 |
| 4.2 EXPERIMENTAL SECTION | 第98-102页 |
| 4.2.1 Preparation of PDAM/HD Coating | 第98页 |
| 4.2.2 S-S and Se-Se Conjugation | 第98-99页 |
| 4.2.3 Preparation of PDAM@Cu coatings | 第99页 |
| 4.2.4 Characterization of the Coatings | 第99页 |
| 4.2.5 NO Catalytic Release | 第99-100页 |
| 4.2.6 Hemocompatibility Evaluation | 第100页 |
| 4.2.7 Smooth muscle cell evaluation | 第100-101页 |
| 4.2.8 Endothelium cell evaluation | 第101页 |
| 4.2.9 Macrophage evaluation and In vivo animal tests | 第101-102页 |
| 4.2.10 Statistical Analysis | 第102页 |
| 4.3 RESULTS AND DISCUSSION | 第102-120页 |
| 4.3.1 Characterization of S-S＠PDAM/HD and Se-Se@PDAM/HD Coatings | 第102-105页 |
| 4.3.2 NO Catalytic Release | 第105-109页 |
| 4.3.3 Hemocompatibility Evaluation | 第109-112页 |
| 4.3.4 Characterization of PDAM@Cu coatings | 第112-114页 |
| 4.3.5 NO Catalytic Release | 第114-115页 |
| 4.3.6 Hemocompatibility and enhanced cGMP expression | 第115-120页 |
| 4.4 CONCLUSION | 第120-122页 |
| CHAPTER 5 SUMMARY AND OUTLOOK | 第122-125页 |
| 5.1 SUMMARY | 第122-123页 |
| 5.2 OUTLOOK | 第123-125页 |
| ACKNOWLEDGEMENTS | 第125-127页 |
| REFERENCES | 第127-143页 |
| ABBREVIATION LIST | 第143-145页 |
| SCIENTIFIC ACHIEVEMENTS | 第145-149页 |
