| Abstract | 第1-23页 |
| Chapter 1 Tissue enzyme catalysis-based optical analytical systems | 第23-71页 |
| ·Introduction | 第23-31页 |
| ·Tissue-based biosensor | 第24-27页 |
| ·Other analytical procedures based on tissue enzyme catalysis | 第27-29页 |
| References | 第29-31页 |
| ·A mushroom tissue-based flow through spectrophotometric system for the determination of catechol and catecholamines | 第31-43页 |
| ·Introduction | 第31-33页 |
| ·Experimental | 第33-35页 |
| ·Reagents and solutions | 第33-34页 |
| ·Apparatus | 第34页 |
| ·Preparation of tissue reactor | 第34-35页 |
| ·Procedures | 第35页 |
| ·Results and discussion | 第35-41页 |
| ·Selection of tissue material | 第35-36页 |
| ·Absorption characteristics of the products | 第36-37页 |
| ·Optimization of reaction conditions | 第37-38页 |
| ·Analytical performance | 第38-39页 |
| ·Selectivity and lifetime of the reactor | 第39页 |
| ·Application | 第39-41页 |
| ·Determination of isoprenaline hydrochloride | 第40页 |
| ·Determination of dopamine | 第40-41页 |
| ·Conclusion | 第41-42页 |
| References | 第42-43页 |
| ·A mushroom tissue-based flow injection fluorescence system for the determination of isoprenaline | 第43-56页 |
| ·Introduction | 第43-46页 |
| ·Experimental | 第46-48页 |
| ·Reagents and Materials | 第46页 |
| ·Apparatus | 第46-47页 |
| ·Preparation of tissue reactor | 第47页 |
| ·Procedure for calibration | 第47-48页 |
| ·Procedure for pharmaceutical preparations | 第48页 |
| ·Results and discussion | 第48-53页 |
| ·Selection of tissue material | 第48页 |
| ·Reaction and spectral characteristics | 第48-49页 |
| ·Conditions for the system | 第49-51页 |
| ·Effect of temperature | 第49页 |
| ·Effect of pH | 第49-50页 |
| ·Effect of concentration of NaOH | 第50页 |
| ·Effect of flow rate | 第50-51页 |
| ·Stability of reagents | 第51页 |
| ·Performance of the proposed system | 第51页 |
| ·Stability and lifetime of the system | 第51-52页 |
| ·Selectivity of the system | 第52页 |
| ·Application | 第52-53页 |
| ·Conclusion | 第53-54页 |
| References | 第54-56页 |
| ·A palnt tissue-bascd flow injection chemiluminescnce system for glutamine | 第56-71页 |
| ·Introduction | 第56-59页 |
| ·Experimental | 第59-62页 |
| ·Reagents and materials | 第59-60页 |
| ·Apparatus | 第60页 |
| ·Preparation of tissue reactor | 第60-61页 |
| ·Procedure for calibration | 第61页 |
| ·Procedure for pharmaceutical preparations | 第61页 |
| ·Procedure for spiked human plasma | 第61-62页 |
| ·Results and discussion | 第62-67页 |
| ·Selection of tissue material of glutaminase | 第62页 |
| ·Conditions for enzymatic reaction in plant tissue column and flow-injection system | 第62-65页 |
| ·Effect of temperature on the tissue reactor and CL intensity | 第62页 |
| ·Selection of pH on the tissue reactor | 第62-63页 |
| ·Effect of flow rate of samples on the system | 第63-64页 |
| ·Effect of NBS concentration on the CL intensity | 第64页 |
| ·Effect of dichlorofluorescein concentration on the CL intensity | 第64页 |
| ·Effect of NaOH concentration on the CL intensity | 第64-65页 |
| ·Analytical characteristics, reproducibility and lifetime of the system | 第65页 |
| ·Selectivity of the system | 第65-66页 |
| ·Applications | 第66-67页 |
| ·Conclusion | 第67-68页 |
| References | 第68-71页 |
| Chapter 2 Investigation on a novel and native bioluminescent fungi-Armillariaella mellea | 第71-103页 |
| ·Introduction | 第71-84页 |
| ·Typical bioluminescence systems and their bioluminescent mechanisms | 第71-76页 |
| ·Application of bioluminescence | 第76-80页 |
| References | 第80-84页 |
| ·An investigation on a novel and native bioluminescent fungi-Armillariaella mellea in gas phase | 第84-95页 |
| ·Introduction | 第84-85页 |
| ·Experimental | 第85-89页 |
| ·Reagents and meterials | 第85-86页 |
| ·Cultivation of strain | 第86-87页 |
| ·Preparation of the reactor | 第87-88页 |
| ·Apparatus | 第88页 |
| ·Procedure | 第88-89页 |
| ·Results and discussions | 第89-94页 |
| ·Selection of culture medium | 第89页 |
| ·Conditions optimization | 第89-90页 |
| ·Effect of media pH | 第89页 |
| ·Effect of temperature | 第89-90页 |
| ·Effect of carrier flow rate | 第90页 |
| ·Effect of sample volume | 第90页 |
| ·Responses to different gases | 第90-91页 |
| ·Response time | 第91页 |
| ·Reproducibility and stability of AM biosensor | 第91-92页 |
| ·Response of the biosensor to O_2 | 第92页 |
| ·Possible bioluminescent mechanisms | 第92-94页 |
| ·Conclusion | 第94页 |
| References | 第94-95页 |
| ·An investigation on a novel and native bioluminescent fungi-Armillariaella mellea in aqueous phase | 第95-103页 |
| ·Introduction | 第95页 |
| ·Experimental | 第95-98页 |
| ·Reagents and strain | 第95-96页 |
| ·Apparatus | 第96页 |
| ·Cultivation of strain | 第96-97页 |
| ·Preparation of the reactor | 第97-98页 |
| ·Preparation of standard sample | 第98页 |
| ·Procedures | 第98页 |
| ·Results and discussion | 第98-101页 |
| ·Selection of culture medium | 第98页 |
| ·Conditions optimization | 第98-100页 |
| ·Effect of media pH | 第98-99页 |
| ·Effect of temperature | 第99页 |
| ·Effect of flow rate of carrier | 第99-100页 |
| ·Effect of sample volume | 第100页 |
| ·Response to DO | 第100页 |
| ·Response time | 第100页 |
| ·Calibration curve | 第100页 |
| ·Reproducibility and stability | 第100页 |
| ·Response to other substances (interference study) | 第100-101页 |
| ·Sample analysis | 第101页 |
| ·Conclusion | 第101-102页 |
| References | 第102-103页 |
| Chapter 3 On-line microdialysis and microultrafiltration sampling coupled with flow-injection chemiluminescence analysis for the study of drug-protein interaction | 第103-141页 |
| ·Introduction | 第103-113页 |
| ·Principle of drug-protein interaction | 第104-106页 |
| ·Methods for studying drug-protein interaction | 第106-110页 |
| References | 第110-113页 |
| ·Flow-injection chemiluminescence detection for studying protein binding of terbutaline sulfate with on-line microdialysis sampling | 第113-127页 |
| ·Introduction | 第113-116页 |
| ·Experimental | 第116-119页 |
| ·Chemicals and reagents | 第116页 |
| ·Apparatus | 第116-117页 |
| ·Procedures | 第117-119页 |
| ·Optimization of CL system | 第117-118页 |
| ·Drug-protein interaction studies in vitro | 第118-119页 |
| ·Results and discussion | 第119-124页 |
| ·Kinetic figure of the CL reaction | 第119页 |
| ·Conditions of the CL detection system | 第119-121页 |
| ·Effect of H_2SO_4 concentration on the CL intensity | 第120页 |
| ·Effect of KMnO_4 concentration on the CL intensity | 第120页 |
| ·Effect of HCHO concentration on the CL intensity | 第120页 |
| ·Effect of flow rate on the CL intensity | 第120-121页 |
| ·Analytical characteristics of the CL system for terbutaline sulfate determination | 第121页 |
| ·Dialytic efficiency calibration of microdialysis probe | 第121-122页 |
| ·Effect of perfusate flow rate on the dialytic efficiency of microdialysis probe | 第121-122页 |
| ·Effect of temperature on the dialytic effciency of microdialysis probe | 第122页 |
| ·Interaction between terbutaline sulfate and BSA | 第122-124页 |
| ·Conclusion | 第124页 |
| References | 第124-127页 |
| ·A flow-injection micro-ultrafiltration sampling Chemiluminescence system for on-line determination of drug-protein interaction | 第127-141页 |
| ·Introduction | 第127-129页 |
| ·Experimental | 第129-132页 |
| ·Chemicals and reagents | 第129-130页 |
| ·Apparatus | 第130-131页 |
| ·Procedures | 第131-132页 |
| ·Optimization of the CL system | 第131-132页 |
| ·Drug-protein interaction studies in vitro | 第132页 |
| ·Results and discussion | 第132-138页 |
| ·Characteristics of the CL reaction | 第132-134页 |
| ·Conditions of the CL detection system | 第134-135页 |
| ·Effect of fluorescein concentration on the CL intensity | 第134页 |
| ·Effect of NaOH concentration on the CL intensity | 第134页 |
| ·Effect of NBS concentration on the CL intensity | 第134-135页 |
| ·Chemiluminescence enhancement by surfactants' | 第135页 |
| ·Effect of flow rate on the CL intensity | 第135页 |
| ·Analytical characteristics of the CL system for cimetidine determination | 第135-136页 |
| ·Interference study | 第136-137页 |
| ·Interaction between cimetidine and BSA | 第137-138页 |
| ·Conclusion | 第138页 |
| References | 第138-141页 |
| Chapter 4 Novel chemiluminescnce systems and the applications in drugs analysis | 第141-217页 |
| ·Introduction | 第141-167页 |
| ·Typical chemiluminscent reaction systems | 第142-147页 |
| ·Strong chemiluminescence systems | 第142-146页 |
| ·Weak chemiluminescence systems | 第146-147页 |
| ·Novel chemiluminscence techniques and trends | 第147-155页 |
| References | 第155-167页 |
| ·Sensitive flow-injection chemiluminescence determination of terbutaline sulfate based on the enhancement of luminol-permanganate reaction | 第167-182页 |
| ·Introduction | 第167-169页 |
| ·Experimental | 第169-172页 |
| ·Reagents | 第169页 |
| ·Apparatus | 第169-170页 |
| ·Procedure for calibration | 第170-171页 |
| ·Procedure for pharmaceutical preparations | 第171页 |
| ·Proposed CL method | 第171页 |
| ·Official method | 第171页 |
| ·Procedure for biological fluids | 第171-172页 |
| ·Determination of terbutaline sulfate in human plasma | 第171-172页 |
| ·Determination of terbutaline sulfate in human urine | 第172页 |
| ·Results and discussion | 第172-179页 |
| ·Kinetic curve of CL reaction | 第172-173页 |
| ·Optimization of the reaction conditions | 第173-174页 |
| ·Effect of NaOH concentration on the CL intensity | 第173页 |
| ·Effect of luminol concentration on the CL intensity | 第173页 |
| ·Effect of KMnO_4 concentration on the CL intensity | 第173页 |
| ·Effect of flow rate on the CL intensity | 第173-174页 |
| ·Effect of sample volume on the CL intensity | 第174页 |
| ·Performance of the proposed method | 第174-175页 |
| ·Interference studies | 第175-176页 |
| ·Application | 第176-178页 |
| ·Analysis of pharmaceutical preparations | 第176页 |
| ·Analysis of spiked plasma and urine sample | 第176-178页 |
| ·Discussion of possible mechanism | 第178-179页 |
| ·Conclusion | 第179页 |
| References | 第179-182页 |
| ·Flow-injection inhibition chemiluminescence determination of indapamide based on luminol-ferricyanide reaction | 第182-193页 |
| ·Introduction | 第182-184页 |
| ·Experimental | 第184-186页 |
| ·Reagents | 第184页 |
| ·Apparatus | 第184-185页 |
| ·Procedure for calibration | 第185页 |
| ·Procedure for pharmaceutical preparations | 第185-186页 |
| ·Results and discussion | 第186-191页 |
| ·Condition optimization of the CL system | 第186-188页 |
| ·Selection of oxidant and the effect of its concentration on the CL intensity | 第186-187页 |
| ·Effect of luminol concentration on the CL intensity | 第187页 |
| ·Effect of NaOH concentration on the CL intensity | 第187-188页 |
| ·Effect of flow rate on the CL intensity | 第188页 |
| ·Effect of Na_2CO_3 on the CL emission | 第188页 |
| ·Performance of the proposed method | 第188页 |
| ·Interference study | 第188-189页 |
| ·Applications | 第189-190页 |
| ·Possible mechanism of the CL system | 第190-191页 |
| ·Conclusion | 第191页 |
| References | 第191-193页 |
| ·Sensitive Flow-injection chemiluminescence determination of metformin based on N-bromosuccinimide-fluorescein system | 第193-205页 |
| ·Introduction | 第193-195页 |
| ·Experimental | 第195-197页 |
| ·Reagents | 第195-196页 |
| ·Apparatus | 第196页 |
| ·Procedure for calibration | 第196-197页 |
| ·Procedure for pharmaceutical preparations | 第197页 |
| ·Results and discussion | 第197-202页 |
| ·Effect of fluorescein concentration | 第197页 |
| ·Effect of sodium hydroxide concentration | 第197-198页 |
| ·Effect of NBS concentration | 第198页 |
| ·Chemiluminescence enhancement by surfactants | 第198-199页 |
| ·Effect of flow rate | 第199页 |
| ·Performance of the proposed method for metformin measurements | 第199-200页 |
| ·Interference study | 第200页 |
| ·Application | 第200-201页 |
| ·Possible mechanism of the chemiluminescence reaction | 第201-202页 |
| ·Conclusion | 第202-203页 |
| References | 第203-205页 |
| ·N-bromosuccinimide-fluorescein based sensitive flow-injection chemiluminescence determination of phenformin | 第205-217页 |
| ·Introduction | 第205-207页 |
| ·Experimental | 第207-209页 |
| ·Reagents | 第207页 |
| ·Apparatus | 第207-208页 |
| ·Procedure | 第208-209页 |
| ·Results and discussion | 第209-215页 |
| ·Characteristics of the CL reaction | 第209-210页 |
| ·Optimization of conditions | 第210-213页 |
| ·Effect of fluorescein concentration | 第210页 |
| ·Effect of sodium hydroxide concentration | 第210-211页 |
| ·Effect of NBS concentration | 第211-212页 |
| ·Chemiluminescence enhancement by surfactants | 第212页 |
| ·Effect of flow rate | 第212-213页 |
| ·Performance of the proposed method for phenformin measurements | 第213页 |
| ·Interference study | 第213-214页 |
| ·Application | 第214-215页 |
| ·Conclusion | 第215页 |
| References | 第215-217页 |
| Publications and presentations | 第217-219页 |
| Acknowledgements | 第219页 |
