| 摘要 | 第1-7页 |
| Abstracts | 第7-11页 |
| Chapter 1 Introduction | 第11-22页 |
| ·An overview of early embryogenesis | 第11页 |
| ·Maternal to zygotic transition in mammals | 第11-13页 |
| ·Reactive oxygen species(ROS) | 第13-17页 |
| ·The functions of oviduct | 第17-19页 |
| ·An overview of co-culture | 第19-21页 |
| ·Existing problems | 第21页 |
| ·Aim of this study | 第21-22页 |
| Chapter 2 Materials and methods | 第22-35页 |
| ·Main equipments | 第22-23页 |
| ·Chemicals and media | 第23-29页 |
| ·Methods | 第29-33页 |
| ·Experimental designs | 第33-34页 |
| ·Statistical analysis | 第34-35页 |
| Chapter 3 Results and analysis | 第35-43页 |
| ·Purification of OE cell line and co-culture | 第35-36页 |
| ·Effect of OECs co-culture on the development of embryos | 第36页 |
| ·Effect of OECs co-culture on the distribution of arrested embryos | 第36-37页 |
| ·Effect of OECs co-culture on the ROS content in early 2-cell embryos | 第37-38页 |
| ·Effect of OECs co-culture on the activity of OECs | 第38-39页 |
| ·Effect of co-culture on the transcriptions of anti-oxidative genes | 第39-40页 |
| ·Selection of oxidative stress concentration of exogenous H_2O_2 | 第40页 |
| ·Effect of oxidative stress on the development of co-cultured embryos | 第40-41页 |
| ·Effect of oxidative stress on the activity of co-cultured OECs | 第41页 |
| ·Detection of apoptosis after H202 treatments | 第41-43页 |
| Chapter 4 Discussion | 第43-46页 |
| ·The establishment of OECs co-culture system | 第43页 |
| ·Effects of OECs co-culture on the development of embryo in vitr #0 | 第43-44页 |
| ·Effect of embryos on the co-cultured OECs | 第44-45页 |
| ·Effect of oxidative stress on the co-culture system | 第45-46页 |
| Chapter 5 Conclusion | 第46-47页 |
| References | 第47-53页 |
| Acknowledgments | 第53-55页 |
| Appendix A(发表论文) | 第55页 |
