| 摘要 | 第3-7页 |
| Abstract | 第7-13页 |
| 学位论文中文提要 | 第14-28页 |
| Chapter 1: Introduction | 第28-40页 |
| 1.1 The ecological significance of marine microbes | 第28-29页 |
| 1.2 Methods for marine microbial ecology studies | 第29-31页 |
| 1.2.1 Traditional methods | 第29-30页 |
| 1.2.2 Molecular methods | 第30页 |
| 1.2.3 High-throughput technologies | 第30-31页 |
| 1.3 Environmental effects of seaweed cultivation on marine ecosystems | 第31-35页 |
| 1.3.1 Status of seaweed cultivation | 第31-33页 |
| 1.3.2 Gracilaria cultivation development in China | 第33-34页 |
| 1.3.3 Bioremediation effects of seaweed cultivation | 第34-35页 |
| 1.4 The research progress in interactions between seaweeds and microbial communities | 第35-37页 |
| 1.4.1 Effects of seaweed cultivation on water and sediment microbial communities | 第35-36页 |
| 1.4.2 Diversity of epiphytes on the seaweed surface | 第36-37页 |
| 1.5 Research significance | 第37-40页 |
| Chapter 2: Cultivation of Gracilaria affects the microbial community structure | 第40-68页 |
| 2.1 Introduction | 第40-41页 |
| 2.2 Materials and methods | 第41-45页 |
| 2.2.1 Sample collections | 第41-43页 |
| 2.2.2 Determination of environmental factors | 第43页 |
| 2.2.3 DNA extraction, amplification and MiSeq sequencing of 16S rRNA gene amplicons | 第43-44页 |
| 2.2.4 Data processing and statistical analysis | 第44-45页 |
| 2.3 Results | 第45-61页 |
| 2.3.1 Effects of G. lemaneiformis cultivation on the water and sediment properties | 第45-47页 |
| 2.3.2 Responses of water and sediment microbial communities to Gracilaria cultivation | 第47-59页 |
| 2.3.3 Linkages between microbial community structure and environmental factors | 第59-61页 |
| 2.4 Discussion | 第61-66页 |
| 2.4.1 Cultivation of Gracilaria benefits the environment | 第61页 |
| 2.4.2 Gracilaria cultivation altered the microbial community structure through changes in environmental factors | 第61-64页 |
| 2.4.3 Certain microbial groups were enriched in response to Gracilaria cultivation | 第64-66页 |
| 2.5 Chapter Summary | 第66-68页 |
| Chapter 3: The dynamics of microbial community structure in Shen‘ao Bay | 第68-94页 |
| 3.1 Introduction | 第68-69页 |
| 3.2 Materials and methods | 第69-72页 |
| 3.2.1 Sampling site and sample collections | 第69-70页 |
| 3.2.2 DNA extraction, amplification and MiSeq sequencing of 16S rRNA gene amplicons | 第70-71页 |
| 3.2.3 Statistical analysis | 第71-72页 |
| 3.3 Results | 第72-89页 |
| 3.3.1 Environmental characteristics in Shen‘ao Bay | 第72-78页 |
| 3.3.2 The dynamics of the microbial community structure in response to Gracilaria cultivation | 第78-87页 |
| 3.3.3 Relationships between environmental factors and the microbial community structure | 第87-89页 |
| 3.4 Discussion | 第89-93页 |
| 3.4.1 Divergences in microbial communities in response to Gracilaria cultivation | 第90-91页 |
| 3.4.2 Gracilaria cultivation alters the microbial succession rate | 第91-93页 |
| 3.5 Chapter Summary | 第93-94页 |
| Chapter 4: The dynamics of functional microbial communities in Shen‘ao Bay | 第94-122页 |
| 4.1 Introduction | 第94-95页 |
| 4.2 Materials and methods | 第95-98页 |
| 4.2.1 Sample collections | 第95-96页 |
| 4.2.2 DNA extraction, purification and labeling | 第96页 |
| 4.2.3 Geo Chip hybridization, scanning and Geo Chip data processing | 第96-97页 |
| 4.2.4 Statistical analysis | 第97-98页 |
| 4.3 Results | 第98-116页 |
| 4.3.1 Temporal dynamics of functional microbial communities | 第98-108页 |
| 4.3.2 Spatial dynamics of functional microbial communities | 第108-116页 |
| 4.4 Discussion | 第116-120页 |
| 4.4.1 The functional microbial community structure in 1-m depth water | 第116-118页 |
| 4.4.2 Gracilaria cultivation shifted the microbial functional gene composition at different depths | 第118-120页 |
| 4.5 Chapter Summary | 第120-122页 |
| Chapter 5: Microbial communities on the G. lemaneiformis surface | 第122-150页 |
| 5.1 Introduction | 第122-123页 |
| 5.2 Materials and methods | 第123-127页 |
| 5.2.1 Sample collection and DNA extraction | 第123-124页 |
| 5.2.2 Amplification of 16S rRNA and ITS genes, and MiSeq sequencing | 第124-125页 |
| 5.2.3 Geo Chip hybridization, scanning and Geo Chip data processing | 第125-126页 |
| 5.2.4 Statistical analysis | 第126-127页 |
| 5.3 Results | 第127-142页 |
| 5.3.1 Overall profiles of the epiphytic and the free-living microbial community of G. lemaneiformis | 第127-128页 |
| 5.3.2 Seaweed and surface-associated microbial groups were enriched in the epiphytic microbial community | 第128-134页 |
| 5.3.3 Microbial community functional protential of G. lemaneiformis epiphytes | 第134-142页 |
| 5.4 Discussion | 第142-148页 |
| 5.4.1 Epiphytic and free-living microbial communities were phylogenetically distinct | 第142-145页 |
| 5.4.2 Epiphytic and free-living microbial communities have divergent functional profiles | 第145-147页 |
| 5.4.3 Linking the diversity, structure and function in the epiphytic communities | 第147-148页 |
| 5.5 Chapter Summary | 第148-150页 |
| Chapter 6：Conclusions, innovations and prospects | 第150-153页 |
| 6.1 Conclusions | 第150页 |
| 6.2 Innovations | 第150-151页 |
| 6.3 Prospects | 第151-153页 |
| References | 第153-168页 |
| 附录 缩写名词对照表 | 第168-170页 |
| 博士研究生期间发表论文及学术交流情况 | 第170-172页 |
| 致谢 | 第172页 |
