| 摘要 | 第1-7页 |
| Abstract | 第7-10页 |
| Part Ⅰ Introduction | 第10-34页 |
| 1. Resonance Rayleigh scattering and its application | 第10-14页 |
| ·Light scattering phenomenon | 第10页 |
| ·Rayleigh scattering | 第10-11页 |
| ·Resonance Rayleigh scattering and its application | 第11-12页 |
| ·Perspective of resonance Rayleigh scattering in analytical chemistry | 第12-14页 |
| 2. Some water pollutants and protein | 第14-18页 |
| ·Malachite green | 第14-15页 |
| ·Lead(Ⅱ)ions | 第15-16页 |
| ·Thrombin | 第16-18页 |
| References | 第18-34页 |
| Part Ⅱ Main research works Section 1.Study on the interaction of malachite green with some heteropoly acids by resonance Rayleigh scattering | 第34-48页 |
| 1. Experamental | 第34-35页 |
| ·Reagents | 第34-35页 |
| ·Apparatus | 第35页 |
| ·General procedure | 第35页 |
| 2. Results and discussion | 第35-46页 |
| ·RRS spectra | 第35-36页 |
| ·Optimum reactive conditions | 第36-39页 |
| ·Sensitivity of method | 第39页 |
| ·Formation of the ion-association complex and its composition ratio | 第39-43页 |
| ·Reasons for RRS enhancement | 第43-44页 |
| ·Selectivity of the Method | 第44-45页 |
| ·An alytical application | 第45-46页 |
| 3. Conclusions | 第46-47页 |
| References | 第47-48页 |
| Section 2.A label-free thrombin binding aptamer as probe for highly sensitive andselective detection of lead(Ⅱ)ions by resonance Rayleigh scattering method | 第48-60页 |
| 1. Experimental | 第48-49页 |
| ·Reagents | 第48-49页 |
| ·Apparatus | 第49页 |
| ·General procedure | 第49页 |
| 2. Results and discussion | 第49-56页 |
| ·RRS spectra | 第49-50页 |
| ·Optimum condition for the reaction system | 第50-52页 |
| ·Sensitivity of the method | 第52-53页 |
| ·Selectivity of the method | 第53-54页 |
| ·The mechanism of the reaction system | 第54-55页 |
| ·Comparison of methods | 第55页 |
| ·Analytical application | 第55-56页 |
| 3. Conclusions | 第56-57页 |
| References | 第57-60页 |
| Section 3. High selective, rapid and facile resonance Rayleigh scattering technique to detect Thrombin using label-free Thrombin binding aptamer | 第60-70页 |
| 1. Experamental | 第60-61页 |
| ·Reagents | 第60-61页 |
| ·Apparatus | 第61页 |
| ·General procedure | 第61页 |
| 2. Results and discussion | 第61-67页 |
| ·RRS spectra | 第61-62页 |
| ·Optimum reactive conditions | 第62-65页 |
| ·Sensitivity of method | 第65页 |
| ·Selectivity of the Method | 第65-66页 |
| ·The mechanism of the TBA aptamer with TB | 第66-67页 |
| 3. Conclusions | 第67-68页 |
| References | 第68-70页 |
| 致谢 | 第70-71页 |
| Appendix:Paper published during the graduate | 第71页 |
