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土壤微生物对铜、镍和矿物浮选捕收剂的单一和交互作用的响应—细胞和群落层面的分析

Acknowledgement第4-6页
摘要第6-8页
Abstract第8-9页
List of abbreviations and symbols第16-17页
1 Introduction第17-22页
    1.1 Research background第17-18页
    1.2 Study interest and motivation第18-20页
    1.3 Research hypotheses and objectives第20-21页
    1.4 Dissertation structure第21-22页
2 Literature review第22-34页
    2.1 Metals occurrence, distribution and fate in the environment第22-30页
        2.1.1 Occurrence and distribution in the environment第22-24页
        2.1.2 Fate in the soil end environment第24-26页
        2.1.3 Effect of metals on the living system第26-28页
        2.1.4 Microbial adaptation to metal toxicity第28-30页
    2.2 Xanthates use and interaction with the living organisms第30-34页
        2.2.1 Xanthates use in flotation and its environmental implication第30-33页
        2.2.2 Interactions between xanthates and the living organisms第33-34页
3 Research content and methodology第34-46页
    3.1 Introduction to methods in soil microbial ecology第34-36页
    3.2 Material第36-37页
        3.2.1 Samples collection and analysis第36-37页
        3.2.2 Reagents第37页
    3.3 Experimentation第37-44页
        3.3.1 Turbidimetric measurement第37-38页
        3.3.2 Spread plating and viable cell count第38-39页
        3.3.3 Microcalorimetric measurement第39页
        3.3.4 Enzymes activities measurement第39-41页
            3.3.4.1 Catalase activity第39-40页
            3.3.4.2 Fluorescein diacetate (FDA) hydrolyases activity第40-41页
        3.3.5 Molecular biology-based analysis第41-44页
    3.4 Data analysis第44-46页
4 Toxic effects of xanthates on soil microbial community and enzymes activities第46-63页
    4.1 Turbidity measurement第46-47页
    4.2 Microbial viable cell count第47-49页
    4.3 Enzymes activity analysis第49-52页
        4.3.1 FDA hydrolyases activity第49页
        4.3.2 Catalase activity第49-50页
        4.3.3 Comparison between FDA hydrolyases and catalase activities第50-52页
    4.4 Discussion第52-62页
    4.5 Summary第62-63页
5 Interactive effects of nickel,copper, and minerals collectors on soil microbialactivity第63-82页
    5.1 Single toxicity of Ni, Cu and minerals collectors第63-69页
    5.2 Interactive effects of Ni and its flotation collectors第69-76页
    5.3 Interactive effects of Cu and xanthates (SIAX and PAX)第76-77页
    5.4 Discussion第77-81页
    5.5 Summary第81-82页
6 Single and interactive effect of nickel,copper and xanthates on the soilmicrobial diversity and structure-Molecular approach第82-108页
    6.1 Bacterial community diversity第82-83页
    6.2 Venn diagram analysis第83-86页
    6.3 Simples distance matrix第86-88页
    6.4 Bacterial community composition第88-96页
    6.5 Discussion第96-101页
    6.6 Traits of potentially nickel and xanthates tolerant bacteria第101-107页
    6.7 Summary第107-108页
7 Conclusions and recommendations第108-113页
    7.1 General conclusions第108-111页
    7.2 Research contribution第111页
    7.3 Recommendation第111-113页
References第113-129页
作者简历及在学研究成果第129-133页
学位论文数据集第133页

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