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中国大鲵ERα、P450arom基因的克隆及不同发育期4种性别决定相关基因的表达分析

摘要第3-7页
Abstract第7-9页
Chapter 1 Review第12-20页
    1 Introduction of the Chinese giant salamander第12-13页
    2 Sexual size dimorphism第13-14页
    3 Introduction of the four genes第14-20页
        3.1 ERα第14-15页
        3.2 P450arom第15-17页
        3.3 Sox9第17-18页
        3.4 Dmrt2第18-20页
Chapter 2 Molecular cloning of ERα and P450arom in the Chinese giantsalamander第20-40页
    1. Material and method第20-26页
        1.1 Materials第20-21页
        1.2 Methods第21-26页
    2. Result第26-35页
        2.1 The result of 3'race第26页
        2.2 Molecular cloning and sequence analysis of the Chinese giant salamander ERα第26-31页
        2.3 Molecular cloning and sequence analysis of Chinese giant salamander P450arom第31-34页
        2.4 Tissue distribution pattern of ERα and P450arom第34-35页
    3. Discussion第35-40页
Chapter 3 Expressions of ERα, P450arom, Sox9 and Dmrt2 during thedevelopment of the Chinese giant salamander第40-60页
    1. Material and method第40-43页
        1.1 Materials第40-41页
        1.2 method第41-43页
    2. Result第43-52页
        2.1 Head-body length,tail length and body weight of the Chinese giant salamander during development第43-44页
        2.2 Histology of the Chinese giant salamander gonads during the development第44-46页
        2.3 Expression of ERα,P450arom,Sox9 and Dmrt2 in gonad,brain and liver of the Chinese giant salamander during development第46-52页
    3. Discussion第52-60页
        3.1 Sexual dimorphism in the Chinese giant salamander第52-53页
        3.2 Possible role of P450arom during development第53-54页
        3.3 Possible role of ERα during development第54-56页
        3.4 Possible role of Sox9 during development第56-57页
        3.5 Possible role of Dmrt2 during development第57-60页
Summarise第60-62页
References第62-76页
致谢第76-78页
攻读硕士期间的研究成果第78页

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