| Abstract | 第1-8页 |
| Table of Content | 第8-12页 |
| Chapter 1 Research background | 第12-21页 |
| ·Introduction | 第12页 |
| ·Review of the literature | 第12-15页 |
| ·Reproduction in the bitch | 第12-13页 |
| ·Endocrine characteristics | 第13-14页 |
| ·Ovarian folliculogenesis,oogenesis and oocyte maturation | 第14-15页 |
| ·Ovarian folliculogenesis and oogenesis | 第14-15页 |
| ·Ovulation and oocyte maturation | 第15页 |
| ·Factors influencing in vitro maturation of canine oocytes | 第15-21页 |
| ·Oocyte size,age and reproductive cycle of bitch and follicle size | 第15-16页 |
| ·Culture condition for IVM of canine oocytes | 第16-18页 |
| ·Culture density | 第16-17页 |
| ·Co-culture | 第17页 |
| ·Culture time | 第17页 |
| ·Oxygen tension and temperature | 第17-18页 |
| ·Culture medium | 第18页 |
| ·The influence of medium supplementation on the IVM of canine oocytes | 第18-21页 |
| ·Growth hormone and growth factors supplementation | 第18-19页 |
| ·Protein supplementation | 第19-20页 |
| ·Antioxidants and energy substrates supplementation | 第20页 |
| ·Other compounds supplementation | 第20-21页 |
| Chapter 2 The influence of 9-cis-retinoic acid on nuclear and cytoplasmic maturation and gene expressionin canine oocytes during in vitro maturation | 第21-31页 |
| ·Introduction | 第21-22页 |
| ·Materials and methods | 第22-26页 |
| ·Collection of canine ovaries and cumulus-oocyte complexes | 第22页 |
| ·In vitro maturation | 第22-23页 |
| ·Removal of cumulus cells and assessment of oocyte nuclear maturation | 第23-24页 |
| ·Labeling of oocytes with fluorescein isothiocyanate(FITC)-lectins for cortical granule(CG) and chromosomal staining | 第24-25页 |
| ·Gene expression of canine cumulus cells | 第25-26页 |
| ·Statistical analysis | 第26页 |
| ·Results | 第26-28页 |
| ·Discussion | 第28-30页 |
| ·Conclusion | 第30-31页 |
| Chapter 3 Influence of ovarian cortex cells co-culture during in vitro maturation on meiotic resumption ofcanine oocytes | 第31-38页 |
| ·Introduction | 第31页 |
| ·Materials and methods | 第31-34页 |
| ·Chemicals and reagents | 第31页 |
| ·Collection of canine ovaries and COCs | 第31-32页 |
| ·Preparation of canine ovarian cortex cells(COCCs)monolayers | 第32-33页 |
| ·In vitro maturation | 第33页 |
| ·Removal of cumulus cells and assessment of oocyte nuclear maturation | 第33-34页 |
| ·Statistical analyses | 第34页 |
| ·Result | 第34-35页 |
| ·Discussion | 第35-36页 |
| ·Conclusion | 第36-38页 |
| Chapter 4 Canine oocyte collection during ryopreservation of the ovarian tissues | 第38-46页 |
| ·Introduction | 第38-39页 |
| ·Materials and methods | 第39-42页 |
| ·Chemicals | 第39页 |
| ·Collection of canine ovaries,oocytes and ovarian tissues | 第39-40页 |
| ·Vitrification and thawing of oocytes | 第40-41页 |
| ·Vitrification and thawing of ovarian tissues | 第41页 |
| ·Analysis of oocyte vitrified-warmed viability | 第41-42页 |
| ·Statistical analysis | 第42页 |
| ·Results | 第42-43页 |
| ·Discussion | 第43-45页 |
| ·Conclusion | 第45-46页 |
| Acknowledgments | 第46-47页 |
| Reference | 第47-59页 |
| Appendix A | 第59-60页 |
| Appendix B | 第60页 |
