| Part I 中文摘要 | 第1-15页 |
| Part II 中文摘要 | 第15-21页 |
| Part I Abstract | 第21-24页 |
| Part II Abstract | 第24-28页 |
| Part I The In Vitro Effects and Mechanism of Low Power LaserIrradiation on Hemortheological Parameters | 第28-78页 |
| Chapter 1 Review | 第28-48页 |
| Review 1 Low power laser therapy | 第28-46页 |
| 1 Introduction | 第28-32页 |
| 2 Biophysics of laser therapy | 第32-34页 |
| 3 Laboratory research and clinica treatment | 第34-40页 |
| ·Cardiac and vascular conditions | 第34-36页 |
| ·Neurological conditions | 第36-38页 |
| ·Wound healing | 第38-40页 |
| ·Pain | 第40-43页 |
| ·Myofascial pain syndrome | 第40页 |
| ·Low back pain | 第40页 |
| ·Patellofemoral pain | 第40-41页 |
| ·Lateral epicondylitis (Tennis elbow) | 第41页 |
| ·Trigger points | 第41页 |
| ·Rotator cuff tendinitis | 第41-43页 |
| 4 Mechanism discussion | 第43-45页 |
| 5 Conclusions | 第45-46页 |
| Review 2: Intravascular low power laser irradiation: clinical use and possible mechanism | 第46-48页 |
| Chapter 2 Materials and methods | 第48-55页 |
| 1 The in vitro effects of low power laser irradiation on hermorheological parameters | 第48-51页 |
| ·Blood samples | 第48页 |
| ·Laser irradiation | 第48-49页 |
| ·For the in vitro effects of Helium Neon laser irradiation on human blood: blood viscosity and deformability of erythrocyte | 第48-49页 |
| ·For the in vitro effects of low power laser irradiation on animal erythrocyte rheology | 第49页 |
| ·For the comparative study of 632.8 and 532 nm laser irradiation on some rheological factors in human blood in vitro | 第49页 |
| ·Measurements of rheological factors | 第49-51页 |
| ·Measurements of hematocrit (HCT) and erythrocyte sedimentation rate | 第49页 |
| ·Measurement of blood viscosity | 第49-50页 |
| ·Measurement of erythrocyte deformability | 第50-51页 |
| ·Measurement of electrophoretic mobility | 第51页 |
| 2 The in vitro effect of low power laser irradiation on membrane attached hemoglobin (Hbm) | 第51-53页 |
| ·Blood preparation | 第51-52页 |
| ·Laser irradiation | 第52页 |
| ·Sample preparation | 第52-53页 |
| ·Hbm induced in erythrocyte ghost | 第52-53页 |
| ·Hbm induced in intact erythrocytes | 第53页 |
| ·Measurements of Hbm | 第53页 |
| 3. Statistical analysis | 第53-55页 |
| Chapter 3 Results and discussion | 第55-76页 |
| 1. The in vitro effects of Helium Neon laser irradiation on human blood: blood viscosity and deformability of erythrocytes | 第55-61页 |
| ·Effect of He-Ne laser irradiation on ESR and HCT | 第55-56页 |
| ·Effect of He-Ne laser irradiation on blood reduced viscosity | 第56-58页 |
| ·Effect of He-Ne laser irradiation on erythrocyte deformability | 第58-60页 |
| ·Effect of He-Ne laser irradiation on erythrocyte deformability (CaCl_2 treated samples) | 第60-61页 |
| 2. In vitro effects of low power laser irradiation on animal erythrocyte's rheology's property | 第61-64页 |
| ·The changes of erythrocyte's deformability after laser irradiation with different intensity (650nm) | 第61-63页 |
| ·Comparative improvements of erythrocyte's deformability after 650nm and 632.8 nm laser irradiation | 第63-64页 |
| ·Changes of mouse erythrocyte's electrophoresis mobility | 第64页 |
| 3 A comparative study of 632.8 and 532 nm laser irradiation on some rheological factors in human blood in vitro | 第64-70页 |
| ·Effects of laser irradiation on HCT and ESR | 第64-65页 |
| ·Effects of laser irradiation on blood visosity | 第65-66页 |
| ·Effects of laser irradiation on electrophoretic mobility of erythrocytes | 第66-67页 |
| ·Effects of laser irradiation on deformability of erythrocytes | 第67-70页 |
| 4 Mechanism of low power laser irradiation on red blood cell deformability | 第70-76页 |
| ·Effects of laser irradiation on Hbm of erythrocyte ghost | 第71-73页 |
| ·Effects of laser irradiation on Hbm of intact erythrocytes | 第73-76页 |
| Chapter 4 Conclusion | 第76-78页 |
| Part II Intersubunit Interaction within the Cytoplasmic Domain of Inwardly Rectifying Potassium Channels Is a Structural Determinant for Channel Activation | 第78-142页 |
| Chapter 1.Review | 第78-113页 |
| Review one: Ion channel, potassium channel and inwardly rectifying potassium channel | 第78-85页 |
| 1. Ion channel | 第78-79页 |
| 2. Potassium channel | 第79-80页 |
| 3. Inward Rectifying Potassium K+ channel | 第80-85页 |
| ·Structure of Kir channels | 第82-84页 |
| ·Kir channels comprise a large superfamily | 第82-83页 |
| ·Primary structure of Kir channels | 第83-84页 |
| ·Chromosomal location | 第84-85页 |
| Review two: Structure and function of ATP-sensitive potassium channels | 第85-113页 |
| 1 Introduction | 第85-89页 |
| 2 Function research of KATP channel | 第89-105页 |
| ·ATPandKir6.2 | 第90-97页 |
| ·Where does Kir6.2 bind ATP? | 第90-91页 |
| ·Mutations affecting ATP sensitivity | 第91-92页 |
| ·Effects of thiol reagents affecting ATP sensitivity | 第92-95页 |
| ·Direct evidence of ATP binding to Kir6.2 | 第95页 |
| ·Mechanism of channel inhibition by ATP | 第95-97页 |
| ·Phosphatidylinositol and Kir6.2 | 第97-104页 |
| ·Physiological coupling between phosphatidylinositol cycle and KATP | 第97页 |
| ·The effects of PPIs on channel function of KATP | 第97-98页 |
| ·Regulation of KATP by PPIs exists in cells: change of membrane PIP2 levels correlates with KATP activity | 第98-99页 |
| ·Mutations affecting PPIs regulation of KATP channel | 第99-100页 |
| ·Electrophysiological characterization of the specificity: Effect of phosphoinsitides varies with headgroup structure | 第100-101页 |
| ·Mechanism of PPI regulation studied by electrophysiological method | 第101-104页 |
| ·Evidence for direct interaction of phosphoinosidies with the channel-forming subunit, Kir6.2 | 第101-102页 |
| ·Cross-interaction between PPI effect and nucleotide2inhibition of KATP: a partial competition hypothesis | 第102-103页 |
| ·The primary site of PPI's modulation effect is on Kir6.2while incorporation of SUR potentiates the modulation | 第103页 |
| ·Implication for specific interaction of PPIs and Kir6.2 | 第103-104页 |
| ·PH and KATP channel | 第104-105页 |
| 3. Structural research of KATP channel | 第105-113页 |
| ·KATP channels are the heteromultimers of SUR and Kir6.2 subunits in which Kir 6.2 forms the pores | 第106-107页 |
| ·Gating of the Kir channels: channel gating kinetics represent two independent gating mechanisms controlling the pore | 第107-109页 |
| ·Intrinsic gating | 第107-108页 |
| ·Controlled gating | 第108-109页 |
| ·Location of the controllable gate | 第109-111页 |
| ·A Hypothetical gating mechanism for Kir channels | 第111-113页 |
| Chapter 2.Materials and methods | 第113-125页 |
| 1. Cell biology techniques: | 第113-114页 |
| ·Cell line and cell culture: | 第113页 |
| ·Protocol for passage of the COS-1 cells | 第113页 |
| ·Protocol for transient tranfection COS-1 cells with mBir and eGFP103 | 第113-114页 |
| 2. Molecular biology techniques | 第114-123页 |
| ·cDNA clones: | 第114页 |
| ·Chimera and fusion protein construction: | 第114页 |
| ·Site-directed mutation, insertion, and deletion: | 第114-121页 |
| ·Heterologous expression in cultured cells: | 第121-122页 |
| ·Reporting and analysis of expression and subcellular distribution: | 第122-123页 |
| ·GFP Florescence | 第122页 |
| ·Double Immunoflorescencestaining and Confocal Microscopy Observation | 第122-123页 |
| 3. Functional assays | 第123-125页 |
| ·Physiological buffers used in the in vitro experiments: | 第123页 |
| ·Patch-clamp recording and analysis of single- or multiple- channel currents: | 第123-124页 |
| ·Monitoring subcellular distribution of PLC-GFP with confocal microscopy: | 第124-125页 |
| Chapter 3 Results and discussion | 第125-140页 |
| 1. Structural models developed describing molecular structure and channel gate | 第125-128页 |
| ·A structural model of the Kir6.2 channel based on the known structures of KirBac 1.1 and Kir3.1 | 第125-126页 |
| ·Involvement of the cytoplasmic domain of Kir6.2 channels in the control of gating | 第126-127页 |
| ·Hypothesi s development | 第127-128页 |
| 2. Functional experiments assessing changes in channel currents in response to structural perturbation | 第128-133页 |
| ·Mutations on channel rundown | 第128-131页 |
| ·Effects of mutations at the selected residues on channel activity in inside-out patches | 第128-129页 |
| ·Statistical summary of the effects of mutations at the selected residues on the rundown process | 第129-130页 |
| ·Statistical summary of the effects of mutational substitutions of V236 on the rundown process in mutant V236 channels | 第130-131页 |
| ·Channel stimulation by ATP | 第131-133页 |
| ·The stimulatory effect is observed upon removal of ATP (inhibition) | 第131-132页 |
| ·Statistical summary comparison of channel stimulation by ATP in mutations in the area of H234 - G243 | 第132-133页 |
| 3. Structural model: possible interpretation | 第133-135页 |
| ·A close-up view of the structural details at the intersubunit interface around Val-236 | 第133-134页 |
| ·A kinetic model interpreting the effects of mutation and ATP | 第134-135页 |
| 4. Mechanisms for channel regulation by cytoplasmic domain interacting signaling molecules: | 第135-140页 |
| ·The stimulatory effect of phosphoinositides is absent in 236S mutant channels, but potentiated in the double mutant | 第136-137页 |
| ·Channel stimulation by acidosis is abbreviated in V236S mutant channel | 第137-140页 |
| ·Cellular acidosis on channel currents of control and mutant channels | 第137-138页 |
| ·Statistical comparison of the effect of cellular acidosis on channel currents in control and mutant channels | 第138-140页 |
| Chapter 4 Summary and Conclusion | 第140-142页 |
| Part I REFERENCES: | 第142-150页 |
| Part II REFERENCES | 第150-159页 |
| PUBLICATIONS | 第159-160页 |
| GRANT FUNDING | 第160页 |
| CONFERENCES | 第160-162页 |
| VITA | 第162-163页 |
| ACKNOWLEDGEMENTS | 第163-166页 |
| 论文独创性声明 | 第166页 |
| 论文使用授权声明 | 第166页 |
