| 摘要 | 第1-6页 |
| ABSTRACT | 第6-8页 |
| Table of Contents | 第8-10页 |
| Chapter 1 Introduction | 第10-24页 |
| ·Background information of Phthalic acid esters (PAEs) | 第10-11页 |
| ·Physiochemical properties and usage of PAEs | 第11-13页 |
| ·Physical and chemical properties of PAEs | 第11-12页 |
| ·Industrial application and commercial uses of PAEs | 第12-13页 |
| ·Contamination of PAEs in the environment | 第13-15页 |
| ·Air pollution | 第13-14页 |
| ·Receiving waters and sediments | 第14-15页 |
| ·Soils | 第15页 |
| ·Toxicity evaluation of PAEs | 第15-16页 |
| ·Abiotic degradation of PAEs in the environment | 第16-17页 |
| ·Hydrolysis | 第16页 |
| ·Photochemical degradation | 第16-17页 |
| ·Biodegradation of PAEs | 第17-19页 |
| ·Biodegradation of PAEs by pure cultures | 第17-18页 |
| ·Biodegradation of PAEs by mixed cultures | 第18-19页 |
| ·Biochemical degradation pathways | 第19-23页 |
| ·Significance and objectives of this study | 第23-24页 |
| Chapter 2 Isolation, identification and diversity of PAEs-degrading bacteria | 第24-39页 |
| ·Introduction | 第24页 |
| ·Material and methods | 第24-28页 |
| ·Chemicals | 第24-25页 |
| ·Source of microorganisms | 第25页 |
| ·Enrichment and isolation of bacteria | 第25页 |
| ·Isolation of genomic DNA and 16S rRNA gene amplification | 第25-26页 |
| ·Sequencing, RFLP and phylogenetic analysis of 16S rRNA gene | 第26页 |
| ·Rep-PCR based DNA fingerprinting and data analysis | 第26-27页 |
| ·Substrate utilizaton test and analytic method | 第27-28页 |
| ·Results | 第28-36页 |
| ·Isolation and characterization of PAEs-degrading bacterial strains | 第28页 |
| ·RFLP and phylogenetic analysis of 16S rRNA gene sequences | 第28-31页 |
| ·Analysis of bacterial diversity based on the basis of rep-PCR | 第31-34页 |
| ·Substrates utilization and DMP degradation by the selected bacteria | 第34-36页 |
| ·Discussion | 第36-38页 |
| ·Summary | 第38-39页 |
| Chapter 3 Complete degradation of di-n-octyl phthalate by biochemical cooperation between | 第39-52页 |
| ·Introduction | 第39-40页 |
| ·Material and methods | 第40-42页 |
| ·Strains | 第40页 |
| ·Further identification and characterization of the two strains | 第40页 |
| ·Substrates utilization tests | 第40页 |
| ·Degradation experiments | 第40-41页 |
| ·Analysis of residual substrates by HPLC | 第41页 |
| ·Analysis of DOP-derived degradation products by GC/MS | 第41-42页 |
| ·Results | 第42-48页 |
| ·Further characterization of the strains | 第42-43页 |
| ·Substrates utilization tests | 第43-44页 |
| ·Degradation of DOP and PA by Gordonia sp. JDC-2 | 第44页 |
| ·Degradation of PA and DOP by Arthrobacter sp. JDC-32 | 第44-45页 |
| ·Complete degradation of DOP by a co-culture of these two strains | 第45-46页 |
| ·GC/MS analysis of DOP-derived degradation products | 第46-48页 |
| ·Discussion | 第48-50页 |
| ·Summary | 第50-52页 |
| Chapter 4 Aerobic biodegradation of diethyl phthalate by Acinetobacter sp. JDC-16 | 第52-62页 |
| ·Introduction | 第52页 |
| ·Material and methods | 第52-54页 |
| ·Strain | 第52页 |
| ·Further identification and characterization of the bacterial strain | 第52-53页 |
| ·Effect of pH and temperature | 第53页 |
| ·Effect of DEP concentrations | 第53页 |
| ·Substrate induction test | 第53-54页 |
| ·Analyic methods | 第54页 |
| ·Results and discussion | 第54-60页 |
| ·Further identification and characterization of the isolate | 第54-55页 |
| ·The optimum DEP degradation conditions by Acinetobacter sp. JDC-16 | 第55-57页 |
| ·Kinetics analysis of DEP degradation | 第57-59页 |
| ·DEP-induction on microbial growth and degradation rate | 第59-60页 |
| ·Summary | 第60-62页 |
| Chapter 5 Conclusions and future perspectives | 第62-65页 |
| ·Conclusions | 第62-63页 |
| ·Future perspectives | 第63-65页 |
| References | 第65-73页 |
| Acknowledgements | 第73-74页 |
| Main achievements | 第74页 |
