| Acronym and Glossary | 第1-10页 |
| PREFACE | 第10-11页 |
| ABSTRACT | 第11-13页 |
| 摘要 | 第13-14页 |
| 1 INTRODUCTION | 第14-34页 |
| 1．1 The Research History of Biomodulation of Monochromatic Visible Light | 第14-22页 |
| 1．1．1 Low Intensity Laser Therapy (LILT) | 第14-16页 |
| 1．1．2 Light emitting diodes | 第16-17页 |
| 1．1．3 Controversial and unestablished mechanisms | 第17-22页 |
| 1．1．3．1 Mitochondrion Theory | 第18-19页 |
| 1．1．3．2 Biological Information Model of Photobiomodulation | 第19-21页 |
| 1．1．3．3 Light irradiation and mitochondria | 第21-22页 |
| 1．2 Molecular Mechanism of Respiratory Burst in Neutrophils | 第22-30页 |
| 1．2．1 The Respiratory Burst of Polymorphonuclear Lukocytes | 第22-23页 |
| 1．2．2 Assemble and Activation of NADPH Oxidase | 第23-26页 |
| 1．2．3 Mechanism of Respiratory Burst | 第26-29页 |
| 1．2．4 NBT test and HVB test about respiratory burst activity of PMNs | 第29-30页 |
| 1．3 Controversial and unestablished mechanisms about apoptosis and Alzheimer's disease | 第30-34页 |
| 1．3．1 What is Alzheimer's Disease? | 第30-31页 |
| 1．3．2 Biomedical research | 第31-32页 |
| 1．3．3 The PC12 cell line | 第32-34页 |
| 2． The effects of Low Intensity Monochromatic Visible Light at different doses on Respiratory Burst in Porcine Neutrophils and their possible mechanism | 第34-44页 |
| 2．1 INTRODUCTION | 第34-35页 |
| 2．2 MATERIALAND METHODS | 第35-38页 |
| 2．2．1 Chemicals and instruments | 第35页 |
| 2．2．2 Neutrophils isolation | 第35-36页 |
| 2．2．3 Irradiation | 第36页 |
| 2．2．4 Assay of Superoxide Production | 第36-37页 |
| 2．2．5 Fluorescent assay of H2O2 generation | 第37-38页 |
| 2．2．6 Immunoblot Analysis | 第38页 |
| 2．3 RESULT | 第38-41页 |
| 2．3．1 Effect of He-Ne laser irradiation at different doses on O~(2-) production by porcine polymorphonucleated neutrophils (PMN) | 第38-39页 |
| 2．3．2 Effect of light emitting diode (LED) irradiation ai different doses on respiratory burst of porcine polymorphonucleated neutrophils (PMN) | 第39-40页 |
| 2．3．3 Fluorescent assay of porcine polymorphonucleated neutrophils (PMN) H2O2 generation | 第40-41页 |
| 2．3．4 Western blot analysis of phosphorylation of PTK、PKC and PLC in porcine PMN | 第41页 |
| 2．4 DISCUSS | 第41-43页 |
| 2．5 CONCLUSION | 第43-44页 |
| 3． LED irradiation decreased the A6 induced apoptosis of differentiated PC12 cells in vitro | 第44-53页 |
| 3．1 INTRODUCTION | 第44页 |
| 3．2 MATERIALAND METHODS | 第44-47页 |
| 3．2．1 Chemicals and instruments | 第44-45页 |
| 3．2．2 Cell cultures | 第45页 |
| 3．2．3 Aβ (25-35) | 第45页 |
| 3．2．4 LED irradiation | 第45-46页 |
| 3．2．5 DNA fragmentation assay | 第46页 |
| 3．2．6 Flow cytometry analysis | 第46-47页 |
| 3．2．7 Fluorimetric analysis of mitochondrial membrane potential (AW) | 第47页 |
| 3．2．8 Data analysis | 第47页 |
| 3．3 RESULTS | 第47-50页 |
| 3．3．1 Experiments on PC12 cells exposed to Aβ(25-35) or / and LED irradiation | 第47-49页 |
| 3．3．2 Analysis of internucleosomal DNA fragmentation | 第49-50页 |
| 3．3．3 Mitochondrial changes upon exposure toAb or, and LED irradiation | 第50页 |
| 3．4 DISCUSSION | 第50-52页 |
| 3．5 CONCLUSION | 第52-53页 |
| Overview | 第53-54页 |
| Publications (published and in preparation) | 第54-55页 |
| 致谢 | 第55-56页 |
| REFERENCE | 第56-70页 |
