| Table of Contents | 第1-9页 |
| List of Figures | 第9-10页 |
| List of Tables | 第10-11页 |
| 摘要 | 第11-13页 |
| Abstract | 第13-16页 |
| INTRODUCTION | 第16-69页 |
| CHARACTERISTICS AND CLASSIFICATION OF CORONAVIRUSES | 第16-27页 |
| Mechanism of Coronavirus Infection | 第18-19页 |
| Receptor Proteins as Determinants of Coronavirus Infection | 第19-21页 |
| Virus Entry | 第21-22页 |
| Pathogenesis | 第22-23页 |
| Cytopathogenesis and Spreading of Progeny | 第23-25页 |
| Species Specificity, Tissue Tropism, and Age-Dependent Resistance | 第25-27页 |
| SEVERE ACUTE RESPIRATORY SYNDROME | 第27-36页 |
| The Outbreak | 第27-29页 |
| Clinical Information | 第29页 |
| Symptoms | 第29页 |
| Investigations | 第29-30页 |
| Diagnostic Tests | 第30页 |
| Diagnosis | 第30-31页 |
| Mortality Rate | 第31页 |
| Treatment | 第31-34页 |
| Current state of Etiologic Knowledge | 第34-36页 |
| GENOME ORGANIZATION OF SARS-COV | 第36-49页 |
| The 5' - Untranslated Region | 第41-42页 |
| The Replicase Gene | 第42-44页 |
| SARS-CoV RNA-Dependent RNA Polymerase | 第44-45页 |
| SARS-CoV Proteinases | 第45-46页 |
| 1. Main Proteinase 3CLpro | 第45-46页 |
| 2. Accessory Proteinase PLpro | 第46页 |
| Single Stranded RNA Binding Protein | 第46-47页 |
| Endoribonuclease | 第47-48页 |
| RNA Helicase | 第48-49页 |
| SARS-COV STRUCTURAL PROTEINS | 第49-58页 |
| Spike protein | 第49-51页 |
| Envelop Protein (E) | 第51-53页 |
| Membrane Protein (M) | 第53-54页 |
| Nucleocapsid Protein (N) | 第54-55页 |
| SARS Coronavirus Accessory Proteins | 第55-56页 |
| The 3' -Stem-Loop II-like Motif (s2m motif) in SARS-CoV | 第56-58页 |
| CORONAVIRUS REPLICATION AND TRANSCRIPTION | 第58-69页 |
| Leader-Primed Transcription | 第61-63页 |
| The Replicon Model | 第63页 |
| Discontinuous Transcription during Negative-Strand RNA Synthesis | 第63-66页 |
| Sequence Elements Regulating Transcription | 第66-69页 |
| OBJECTIVES | 第69-70页 |
| MATERIALS AND METHODS | 第70-80页 |
| Cells, Viruses, Antibodies and Bacteria | 第70-71页 |
| In Silico Analysis | 第71页 |
| Construction of Plasmids | 第71-74页 |
| Northern Blot | 第74-75页 |
| Transfection and Western Blotting | 第75-76页 |
| General Molecular-Biology Techniques | 第76页 |
| Extraction of RNA from Virus Infected Cells | 第76-77页 |
| Synthesis of Viral cDNAs | 第77页 |
| Polymerase Chain Reactions (PCR) | 第77-78页 |
| Agarose Gel Electrophoresis for Nucleic Acids | 第78页 |
| Ligation, Transformation and Screening for Positive Clones | 第78-80页 |
| RESULTS | 第80-104页 |
| Features of the Genomic Structure of SARS-CoV Isolate WHU | 第80-84页 |
| Co-existence of both Positive and Negative-Strand Subgenomic RNA in SARS-CoV Infected Cells | 第84-89页 |
| Identification of Novel Subgenomic RNAs | 第89-94页 |
| Subgenomic RNAs 2-1 and 3-1 could be Functional Messages | 第94-96页 |
| Expression Profile of SARS-CoV Subgenomic RNAs | 第96-99页 |
| Role of 5'-UTR in Viral Translation | 第99-102页 |
| Uniqueness and Stability of the Junction Sequences of each Subgenomic RNAs | 第102-104页 |
| DISCUSSION | 第104-117页 |
| REFERENCES | 第117-149页 |
| ACKNOWLEDGMENT | 第149-151页 |
| Papers Published to be Published During the PhD Study(博士研究生期间发表和待发表的论文:) | 第151页 |
