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类病毒的遗传变异及结构功能分析

Abstract第4页
摘要第5-8页
1 Introduction第8-22页
    1.1 Viroids第8-13页
        1.1.1 Classification and structure第8-11页
        1.1.2 Replication and movement第11-13页
    1.2 Viroids and hosts第13-15页
        1.2.1 Differences in host range of viroids第13-14页
        1.2.2 Host specific variants第14-15页
    1.3 Detection methods for viroids第15-20页
        1.3.1 Biological assays第15页
        1.3.2 Polyacrylamide gel electrophoresis (PAGE)第15-16页
        1.3.3 Nucleic acid hybridization第16-18页
        1.3.4 Reverse Transcription PCR (RT-PCR)第18-20页
        1.3.5 PFOR approach第20页
    1.4 Purpose and significance of the present study第20-22页
2 Genetic variation of CbVds and a possible new viroid第22-45页
    2.1 Introduction第22-24页
    2.2 Methods第24-35页
        2.2.1 Construction of infectious clones and inoculation第24页
        2.2.2 RNA extraction第24-25页
        2.2.3 2D-PAGE and hybridization第25-32页
        2.2.4 Cloning and sequencing第32-35页
    2.3 Results and discussion第35-43页
        2.3.1 Biological assay of CbVds第35-36页
        2.3.2 Sequencing analysis of CbVds progenies第36-40页
        2.3.3 High-throughput sequencing revealed infection of viroids第40-41页
        2.3.4 Proposed secondary structure of CbVd-X第41页
        2.3.5 CbVd-X is common in coleus samples第41-43页
    2.4 Conclusion第43-45页
3 Functional analysis of a viroid RNA motif mediating cell to cell movement第45-63页
    3.1 Introduction第45-47页
    3.2 Methods第47-56页
        3.2.1 cDNA construction of PSTVd mutants and infection第47页
        3.2.2 RNA extraction第47页
        3.2.3 Cloning and sequencing第47-50页
        3.2.4 Hybridization第50-56页
        3.2.5 Quantitative analysis of PSTVd-accumulating cells第56页
    3.3 Results and discussion第56-63页
        3.3.1 Influence of RNA probes of different lengths第56-57页
        3.3.2 Replication efficiency of loop 19 mutants第57-59页
        3.3.3 Progenies of loop 19 mutants第59-61页
        3.3.4 Loop 19 is required for PSTVd trafficking第61-62页
        3.3.5 Conclusion第62-63页
4 Discussion for the full paper第63-64页
    4.1 Genetic variation of CbVds and a possible new viroid in Coleus blumei第63页
    4.2 Functional analysis of a viroid RNA motif mediating cell to cell movement第63-64页
5 Acknowledgements第64-65页
6 Reference第65-73页
7 Curriculum vitae第73页

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