| LIST OF ABBREVIATIONS USED | 第8-9页 |
| ABSTRACT | 第9-10页 |
| LIST OF TABLES | 第15-16页 |
| LIST OF FIGURES | 第16-18页 |
| 1. Introduction | 第18-19页 |
| 2. Statement of the Problem | 第19-20页 |
| 3. Research Questions | 第20页 |
| 4. Objectives | 第20-21页 |
| 5. Jusntification | 第21-22页 |
| 6. Part Ⅰ:detecting MBD2 protein and mRNA | 第22-31页 |
| 6.1 Materials | 第22-24页 |
| 6.2 Methods | 第24-28页 |
| 6.2.1 Cell cultures | 第24页 |
| 6.2.2 Protein preparation (Extraction) | 第24-25页 |
| 6.2.3 Western blotting | 第25-26页 |
| 6.2.4 Real-Time PCR | 第26-28页 |
| 6.3 Results | 第28-30页 |
| 6.3.1 Expression MBD2 protein by several hematopoietic cell lines using western blotting | 第28-29页 |
| 6.3.2 Expression of MBD2-mRNA by using real-time PCR | 第29-30页 |
| 6.4 Discussion | 第30-31页 |
| 7. Part Ⅱ:MBD2-Knockdown, Cell cycle, Apoptosis, and Colony Forming Cell Assay | 第31-75页 |
| 7.1 Materials | 第31-34页 |
| 7.2 Methods | 第34-40页 |
| 7.2.1 Lentivirus shRNA-MBD2 analysis and Checking | 第34页 |
| 7.2.2 MBD2 knocking down | 第34-35页 |
| 7.2.3 Western blot to detect MBD2 knocking down | 第35-37页 |
| 7.2.4 SYBR Green fluorescence based quantitative real-time PCR to detect MBD2 knocking down | 第37-38页 |
| 7.2.5 Cell cycle Analysis of shRNA-MBD2-Knocked down Cells | 第38-39页 |
| 7.2.6 Apoptosis analyses of shRNA-MBD2-Knocked down Cells | 第39页 |
| 7.2.7 Colony forming assay of sh-RNA-MBD2 knocked down cells | 第39-40页 |
| 7.3 Results | 第40-72页 |
| 7.3.1 Result of Lentivirus shRNA-MBD2 analysis and Checking | 第40-43页 |
| 7.3.2 Result of Western blotting to detect MBD2 knocking down | 第43-45页 |
| 7.3.3 Result of SYBR Green fluorescence based quantitative real-time PCR to detect MBD2 knocking down | 第45-47页 |
| 7.3.4 Result of Cell cycle Analysis of shRNA-MBD2-Knocked down Cells | 第47-58页 |
| 7.3.5 Result of apoptosis analyses of Lentivirus shRNA-MBD2-Knocked Down Cells Lines | 第58-68页 |
| 7.3.6 Results of Colony Forming cell Assay | 第68-72页 |
| 7.4 Discussion | 第72-75页 |
| 8 Part Ⅲ:MBD2 Protein Role in Increasing response of Hematopoietic Malignancies to Treatment | 第75-83页 |
| 8.1 Apoptosis Analysis of Treated THP-1Cell Line with DNR Treatment | 第75-76页 |
| 8.2 Cell Cycle Analysis of Treated Cell Line THP-1 with DNR | 第76-77页 |
| 8.3 Results of apoptosis analysis of treated THP-1 with DAR | 第77页 |
| 8.4 Results of Cell Cycle analysis of treated THP-1 with DAR | 第77-82页 |
| 8.5 Discussion of Part Ⅲ | 第82-83页 |
| 9 Review:Myelodysplastic syndrome (MDS) | 第83-84页 |
| 10 Pathogenesis of Myelodysplastic Syndrome (MDS) | 第84-86页 |
| 11 Genetic Alterations in Signal Transduction and Cell Surface Receptors | 第86页 |
| 12 Alterations of Transcription Factors and Cell Cycle Regulators | 第86-89页 |
| 13 Spliceosome Mutations in Myelodysplastic Syndrome (MDS) | 第89-91页 |
| 14 Epigenetic Alterations in MDS | 第91-95页 |
| 15 Cytogenetic Abnormalities in MDS | 第95-97页 |
| 16 Classification and Prognostic Scoring Systems of MDS | 第97-100页 |
| 17 Natural History and Goals of Treatment of MDS | 第100-101页 |
| 18 Treatment of MDS | 第101页 |
| 19 Hematopoietic Cell Transplantation (HCT) of MDS | 第101-102页 |
| 20 Epigenetic Treatment Strategy of MDS | 第102-103页 |
| 21 DNA Demethy lating Agents | 第103-104页 |
| 22 Histone Deacetylase Inhibitors | 第104页 |
| 23 Combination Strategies | 第104-106页 |
| 24 Epigenetic | 第106-107页 |
| 25 Modification of Histone and Chromatin Function | 第107-108页 |
| 26 DNA Methylation | 第108-109页 |
| 27 Methylation Enzymes | 第109-110页 |
| 28 Methylation in Human Development | 第110-112页 |
| 29 Methylation and Cancer | 第112-113页 |
| 30 Role of Methylation Therapy in Treatment | 第113页 |
| 31 The Mixed Lineage Leukemia (MLL) rearrangements | 第113-114页 |
| 32 MLL Protein Structure | 第114-117页 |
| 33 MLL in Normal Hematopoiesis | 第117页 |
| 34 Disordered Epigenetic Regulation in MLL Fusions | 第117-118页 |
| 35 Target Genes | 第118-120页 |
| 36 Disordered Epigenetic Regulation in MLL-Partial Tandem Duplication (MLL-PTD) | 第120-121页 |
| 37 Methyl-Binding Domain Proteins and its Role in Tumorigenesis | 第121-123页 |
| 38 MBD Family Structure | 第123-124页 |
| 39 Literature review | 第124-138页 |
| 40 References | 第138-148页 |
| ACKNOWLEDGMENT | 第148-149页 |
