| 摘要 | 第6-10页 |
| ABSTRACT | 第10-11页 |
| Chapter 1 Introduction | 第16-20页 |
| 1.1 Background | 第16-17页 |
| 1.2 Specific objectives of this study | 第17-18页 |
| 1.3 Scope of the study | 第18-19页 |
| 1.4 Technical map | 第19-20页 |
| Chapter 2 Review of Literature | 第20-34页 |
| 2.1 Overview of Cr and Pb | 第20-22页 |
| 2.2 Common technologies used for heavy metals removal | 第22-26页 |
| 2.2.1 Physico-chemical methods | 第22-23页 |
| 2.2.2 Bioremediation and phytoremediation | 第23-26页 |
| 2.3 Biomineralization based remediation methods | 第26-29页 |
| 2.3.1 Biomineralization and microbially induced calcite precipitation (MICP) | 第26-27页 |
| 2.3.2 Fungal role in ureolytic biomineralization and its potential in heavy metaltreatment | 第27-29页 |
| 2.4 Mechanism of MICP process | 第29-34页 |
| 2.4.1 Factors affecting the efficiency of MICP | 第31-34页 |
| Chapter 3 Materials and Methods | 第34-45页 |
| 3.1 Materials and chemicals preparation | 第34-35页 |
| 3.2 Isolation and identification of fungi | 第35-36页 |
| 3.2.1 Isolation of ureolytic fungi | 第35页 |
| 3.2.2 Identification of fungal strain | 第35-36页 |
| 3.3 Optimization of medium and condition for fungal growth and urease activity | 第36-38页 |
| 3.4 Experimental set for bioremediation of heavy metals at flask level | 第38-39页 |
| 3.5 Experimental set for bioremediation of heavy metals from soil | 第39-40页 |
| 3.6 Analytical and determination methods | 第40-45页 |
| 3.6.1 Measurement of pH, dry weight of mycelia, and soil porosity | 第40-41页 |
| 3.6.2 Urease activity assay | 第41-42页 |
| 3.6.3 Heavy metal extraction and concentration determination methods | 第42-43页 |
| 3.6.4 Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction(XRD) | 第43-44页 |
| 3.6.5 Optical and scanning electron microscopy (SEM/EDS) | 第44页 |
| 3.6.6 Statistical method | 第44-45页 |
| Chapter 4 Results and Discussions | 第45-73页 |
| 4.1 Isolation and identification of Cr resistant fungi | 第45-46页 |
| 4.2 Optimization of factors on fungal growth and urease activities | 第46-49页 |
| 4.3 Bioremediation of Cr(Ⅵ) and Pb at flask level | 第49-68页 |
| 4.3.1 Pre-experiment on heavy metals removal | 第49-50页 |
| 4.3.2 Heavy metals removal by P. chrysogenum CS1 via MICP treatment | 第50-54页 |
| 4.3.3 Morphology analyses of fungal mycelia and biominerals | 第54-60页 |
| 4.3.4 FTIR and XRD spectra based mineralogy analyses on fungal mycelia andbiominerals | 第60-68页 |
| 4.4 Bioremediation of Cr and Pb from soil | 第68-73页 |
| Chapter 5 Conclusions | 第73-76页 |
| References | 第76-83页 |
| Acknowledgements | 第83页 |
