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Evaluation of N-carbamoylglutamate as Arginine Enhancer and Its Effect on Rumen Fermentation,Lactation Performance and Nitrogen Utilization in High Yielding Lactating Dairy Cows

DEDICATION第7-8页
Table of Content第8-11页
List of Tables第11-12页
List of ifgures第12-13页
ABBREVIATION LIST第13-15页
摘要第15-19页
ABSTRACT第19-23页
CHAPTER-1 GENERAL INTRODUCTION第24-28页
CHAPTER-Ⅱ REVIEW OF LITERATURE第28-48页
    2.1 L-Arginine function, chemistry, source, metabolism, mode of action and its importance in ruminant animal health and production第28-33页
    2.2 Mechanism of biosynthesis of Arg by NCG supplementation in ruminant animals第33-35页
    2.3 Significance of NCG in dairy animal's production第35-45页
        2.3.1 Lactation第35-37页
        2.3.2 Nitrogen utilization第37-41页
        2.3.3 Significance of N-carbamoylglutamate in reproduction第41-44页
        2.3.4 Effect of NCG on growth and cell proliferation第44-45页
    Summary第45-48页
CHAPTER-Ⅲ Development of methods for the determination of Arginine andN-carbamoylglutamate in rumen fluid第48-64页
    3.1 ABSTRACT第48-49页
    3.2 INTRODUCTION第49-50页
    3.3 MATERIALS AND METHODS第50-54页
        3.3.1 Experimental Design第50页
        3.3.2 Method of Arg and NCG determination in rumen fluid in invtro第50-51页
        3.3.3 Chemical analytical procedures of Arg and NCG第51-53页
        3.3.4 Setup of standard line for Arg and NCG determination第53-54页
        3.3.5 Data Calculation and Statistical analysis第54页
    3.4 RESULTS第54-58页
        3.4.1 Effect of temperature and time on colour development and determination stability of Arg in rumen fluid第54-55页
        3.4.2 Absorptive wavelength第55页
        3.4.3 Determination of Arg concentration in rumen fluid第55-56页
        3.4.4 Determination of NCG in rumen fluid第56-58页
    3.5 DISCUSSION第58-64页
        3.5.0 Effect of temperature and time on colour development and determination stability第59-60页
        3.5.1 Absorptive wavelength第60页
        3.5.2 Determination of Arg concentration in rumen fluid第60-61页
        3.5.3 Determination of NCG in rumen fluid第61-64页
CHAPTER-Ⅳ Evaluation of Arginine and N-carbamoylglutamate degradation in rumen and theireffect on rumen fermentation in vitro第64-74页
    4.1 ABSTRACT第64页
    4.2 INTRODUCTION第64-66页
    4.3 MATERIALS AND METHODS第66-69页
        4.3.1 Experimental design第66页
        4.3.2 In vitro incubation第66-67页
        4.3.3 Measurement of in vitro fermentation variables第67-68页
        4.3.4 Analysis of L-arg and NCG in rumen fluid第68页
        4.3.5 Data calculation and statistical analysis第68-69页
    4.4 RESULTS第69-70页
        4.4.1 Degradation of L-arg and NCG in rumen fluid第69-70页
        4.4.2 Effects on gas production and fermentation variables第70页
    4.5 DISCUSSION第70-74页
        4.5.1 Degradation of L-arg and NCG in rumen fluid第70-71页
        4.5.2 Effects on gas production and fermentation variables第71-74页
CHAPTER-Ⅴ The effects of N-carbamoylglutamate on milk production and nitrogen utilization ofhigh yielding Chinese Holstein dairy cows第74-98页
    5.1 ABSTRACT第74-75页
    5.2 INTRODUCTION第75-76页
    5.3 MATERIALS AND METHODS第76-82页
        5.3.1 Animals, diet and experiment design第76-77页
        5.3.2 Sampling, Measurement, and Analysis第77-80页
        5.3.3 In Situ Digestion in the Rumen第80-81页
        5.3.4 Estimation of MCP yield on PD excretion and Metabolizable Protein第81-82页
        5.3.5 Statistical Analysis第82页
    5.4 RESULTS第82-87页
        5.4.1 DMI and Milk Performance第82-83页
        5.4.2 Plasma Variables第83-84页
        5.4.3 Plasma AA profile and N Utilization Efficiency第84-85页
        5.4.4 Effect on MCP synthesis in rumen and metabolizable protein utilization第85-87页
    5.5 DISCUSSION第87-98页
        5.5.1 Effect on DMI and Milk第87-90页
        5.5.2 Effect on plasma AA concentration第90-93页
        5.5.3. Effect on plasma variables第93-94页
        5.5.4 Effect on N utilization第94-96页
        5.5.5. Effect on MCP synthesis in the rumen第96-97页
        5.5.6. Effect on MP Utilization第97-98页
CHAPTER-VI SUMMARY AND CONCLUSIONS第98-100页
INNOVATION第100-102页
REFERENCES第102-122页
ACKNOWLEDGMENTS第122-124页
CURRICULUM VITAE (CV)第124-125页

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