DEDICATION | 第7-8页 |
Table of Content | 第8-11页 |
List of Tables | 第11-12页 |
List of ifgures | 第12-13页 |
ABBREVIATION LIST | 第13-15页 |
摘要 | 第15-19页 |
ABSTRACT | 第19-23页 |
CHAPTER-1 GENERAL INTRODUCTION | 第24-28页 |
CHAPTER-Ⅱ REVIEW OF LITERATURE | 第28-48页 |
2.1 L-Arginine function, chemistry, source, metabolism, mode of action and its importance in ruminant animal health and production | 第28-33页 |
2.2 Mechanism of biosynthesis of Arg by NCG supplementation in ruminant animals | 第33-35页 |
2.3 Significance of NCG in dairy animal's production | 第35-45页 |
2.3.1 Lactation | 第35-37页 |
2.3.2 Nitrogen utilization | 第37-41页 |
2.3.3 Significance of N-carbamoylglutamate in reproduction | 第41-44页 |
2.3.4 Effect of NCG on growth and cell proliferation | 第44-45页 |
Summary | 第45-48页 |
CHAPTER-Ⅲ Development of methods for the determination of Arginine andN-carbamoylglutamate in rumen fluid | 第48-64页 |
3.1 ABSTRACT | 第48-49页 |
3.2 INTRODUCTION | 第49-50页 |
3.3 MATERIALS AND METHODS | 第50-54页 |
3.3.1 Experimental Design | 第50页 |
3.3.2 Method of Arg and NCG determination in rumen fluid in invtro | 第50-51页 |
3.3.3 Chemical analytical procedures of Arg and NCG | 第51-53页 |
3.3.4 Setup of standard line for Arg and NCG determination | 第53-54页 |
3.3.5 Data Calculation and Statistical analysis | 第54页 |
3.4 RESULTS | 第54-58页 |
3.4.1 Effect of temperature and time on colour development and determination stability of Arg in rumen fluid | 第54-55页 |
3.4.2 Absorptive wavelength | 第55页 |
3.4.3 Determination of Arg concentration in rumen fluid | 第55-56页 |
3.4.4 Determination of NCG in rumen fluid | 第56-58页 |
3.5 DISCUSSION | 第58-64页 |
3.5.0 Effect of temperature and time on colour development and determination stability | 第59-60页 |
3.5.1 Absorptive wavelength | 第60页 |
3.5.2 Determination of Arg concentration in rumen fluid | 第60-61页 |
3.5.3 Determination of NCG in rumen fluid | 第61-64页 |
CHAPTER-Ⅳ Evaluation of Arginine and N-carbamoylglutamate degradation in rumen and theireffect on rumen fermentation in vitro | 第64-74页 |
4.1 ABSTRACT | 第64页 |
4.2 INTRODUCTION | 第64-66页 |
4.3 MATERIALS AND METHODS | 第66-69页 |
4.3.1 Experimental design | 第66页 |
4.3.2 In vitro incubation | 第66-67页 |
4.3.3 Measurement of in vitro fermentation variables | 第67-68页 |
4.3.4 Analysis of L-arg and NCG in rumen fluid | 第68页 |
4.3.5 Data calculation and statistical analysis | 第68-69页 |
4.4 RESULTS | 第69-70页 |
4.4.1 Degradation of L-arg and NCG in rumen fluid | 第69-70页 |
4.4.2 Effects on gas production and fermentation variables | 第70页 |
4.5 DISCUSSION | 第70-74页 |
4.5.1 Degradation of L-arg and NCG in rumen fluid | 第70-71页 |
4.5.2 Effects on gas production and fermentation variables | 第71-74页 |
CHAPTER-Ⅴ The effects of N-carbamoylglutamate on milk production and nitrogen utilization ofhigh yielding Chinese Holstein dairy cows | 第74-98页 |
5.1 ABSTRACT | 第74-75页 |
5.2 INTRODUCTION | 第75-76页 |
5.3 MATERIALS AND METHODS | 第76-82页 |
5.3.1 Animals, diet and experiment design | 第76-77页 |
5.3.2 Sampling, Measurement, and Analysis | 第77-80页 |
5.3.3 In Situ Digestion in the Rumen | 第80-81页 |
5.3.4 Estimation of MCP yield on PD excretion and Metabolizable Protein | 第81-82页 |
5.3.5 Statistical Analysis | 第82页 |
5.4 RESULTS | 第82-87页 |
5.4.1 DMI and Milk Performance | 第82-83页 |
5.4.2 Plasma Variables | 第83-84页 |
5.4.3 Plasma AA profile and N Utilization Efficiency | 第84-85页 |
5.4.4 Effect on MCP synthesis in rumen and metabolizable protein utilization | 第85-87页 |
5.5 DISCUSSION | 第87-98页 |
5.5.1 Effect on DMI and Milk | 第87-90页 |
5.5.2 Effect on plasma AA concentration | 第90-93页 |
5.5.3. Effect on plasma variables | 第93-94页 |
5.5.4 Effect on N utilization | 第94-96页 |
5.5.5. Effect on MCP synthesis in the rumen | 第96-97页 |
5.5.6. Effect on MP Utilization | 第97-98页 |
CHAPTER-VI SUMMARY AND CONCLUSIONS | 第98-100页 |
INNOVATION | 第100-102页 |
REFERENCES | 第102-122页 |
ACKNOWLEDGMENTS | 第122-124页 |
CURRICULUM VITAE (CV) | 第124-125页 |