Declaration | 第3-4页 |
Certification | 第4-5页 |
Dedication | 第5-6页 |
Acknowledgements | 第6-8页 |
Table of contents | 第8-11页 |
List of figures | 第11-13页 |
List of tables | 第13-14页 |
Abbreviations | 第14-15页 |
Unit abbreviations | 第15-16页 |
英文摘要 | 第16页 |
1 Introduction | 第18-21页 |
2 Literature review | 第21-35页 |
2.1 Induced disease resistance in plants | 第21-24页 |
2.1.1 Historic and Concept | 第21-22页 |
2.1.2 Mechanism of induced plant resistance | 第22-24页 |
2.2 Microbially induced plant resistance | 第24-26页 |
2.3 The possible mechanisms of Trichoderma species in Biological control | 第26-28页 |
2.3.1 Generalities on the Genus Trichoderma and ecological distribution | 第26页 |
2.3.2 Mycoparasitism | 第26-27页 |
2.3.3 Competition | 第27页 |
2.3.4 Antibiosis | 第27页 |
2.3.5 Lysis | 第27-28页 |
2.4 The induced resistance of plants by Trichoderma species | 第28-31页 |
2.5 Application of induced resistance | 第31-32页 |
2.6 Important enzymes involved in biosynthesis of antimicrobial compounds | 第32-35页 |
2.6.1 Role of Peroxidase in disease resistance | 第32-33页 |
2.6.2 The role of phenylalanine ammonia-lyase in plant disease resistance | 第33-35页 |
3 Materials and Methods | 第35-42页 |
3.1 Antagonism in vitro of Trichoderma harzianum against Magnaporthe grisea and Xanthomonas oryzae pv. oryzae | 第35-37页 |
3.1.1 Tested isolates | 第35页 |
3.1.2 Media | 第35-36页 |
3.1.2.1 PDA (Potato Dextrose Agar) | 第35页 |
3.1.2.2 Yeast extract agar | 第35页 |
3.1.2.3 Media for culturing Xantomonas oryzae pv oryzae | 第35页 |
3.1.2.4 Sterilization of the culture media | 第35-36页 |
3.1.3 Dual culture test | 第36页 |
3.1.4 Filter culture test | 第36-37页 |
3.2 Greenhouse tests | 第37-40页 |
3.2.1 Plant materials | 第37页 |
3.2.2 Media | 第37页 |
3.2.3 Preparation of inoculum of pathogens | 第37-38页 |
3.2.4 Trichoderma inoculum preparation | 第38页 |
3.2.5 Seed preparation and rice cultivation | 第38页 |
3.2.6 Rice blast inoculation and scoring systems | 第38-39页 |
3.2.7 Bacterial blight inoculation and scoring | 第39-40页 |
3.3 Enzyme activity assay | 第40-42页 |
3.3.1 Assay of peroxidase (POD) activity | 第40-41页 |
3.3.2 Assay of Phenylalanine ammonia lyase (PAL) activity | 第41页 |
3.3.3 Protein determination | 第41-42页 |
4 RESULTS | 第42-62页 |
4.1 ”IN VITRO” Antagonistic tests of Trichoderma harzianum NF9 against the rice pathogens | 第42-50页 |
4.1.1 Dual culture | 第42-46页 |
4.1.2 The filter culture | 第46-50页 |
4.2 Greenhouse experiments | 第50-57页 |
4.3 Enzyme activity results | 第57-62页 |
5 Discussion | 第62-68页 |
5.1 In vitro antagonistic activity of T. harzianum NF9 against the rice pathogens | 第62-63页 |
5.2 The induced resistance of rice by Trichoderma | 第63-64页 |
5.3 Enzyme activity involved in the induced resistance of rice by Trichoderma | 第64-68页 |
6 Conclusion and perspectives | 第68-70页 |
References | 第70-84页 |