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Induction of Disease Resistance of Rice by Trichoderma Species

Declaration第3-4页
Certification第4-5页
Dedication第5-6页
Acknowledgements第6-8页
Table of contents第8-11页
List of figures第11-13页
List of tables第13-14页
Abbreviations第14-15页
Unit abbreviations第15-16页
英文摘要第16页
1 Introduction第18-21页
2 Literature review第21-35页
    2.1 Induced disease resistance in plants第21-24页
        2.1.1 Historic and Concept第21-22页
        2.1.2 Mechanism of induced plant resistance第22-24页
    2.2 Microbially induced plant resistance第24-26页
    2.3 The possible mechanisms of Trichoderma species in Biological control第26-28页
        2.3.1 Generalities on the Genus Trichoderma and ecological distribution第26页
        2.3.2 Mycoparasitism第26-27页
        2.3.3 Competition第27页
        2.3.4 Antibiosis第27页
        2.3.5 Lysis第27-28页
    2.4 The induced resistance of plants by Trichoderma species第28-31页
    2.5 Application of induced resistance第31-32页
    2.6 Important enzymes involved in biosynthesis of antimicrobial compounds第32-35页
        2.6.1 Role of Peroxidase in disease resistance第32-33页
        2.6.2 The role of phenylalanine ammonia-lyase in plant disease resistance第33-35页
3 Materials and Methods第35-42页
    3.1 Antagonism in vitro of Trichoderma harzianum against Magnaporthe grisea and Xanthomonas oryzae pv. oryzae第35-37页
        3.1.1 Tested isolates第35页
        3.1.2 Media第35-36页
            3.1.2.1 PDA (Potato Dextrose Agar)第35页
            3.1.2.2 Yeast extract agar第35页
            3.1.2.3 Media for culturing Xantomonas oryzae pv oryzae第35页
            3.1.2.4 Sterilization of the culture media第35-36页
        3.1.3 Dual culture test第36页
        3.1.4 Filter culture test第36-37页
    3.2 Greenhouse tests第37-40页
        3.2.1 Plant materials第37页
        3.2.2 Media第37页
        3.2.3 Preparation of inoculum of pathogens第37-38页
        3.2.4 Trichoderma inoculum preparation第38页
        3.2.5 Seed preparation and rice cultivation第38页
        3.2.6 Rice blast inoculation and scoring systems第38-39页
        3.2.7 Bacterial blight inoculation and scoring第39-40页
    3.3 Enzyme activity assay第40-42页
        3.3.1 Assay of peroxidase (POD) activity第40-41页
        3.3.2 Assay of Phenylalanine ammonia lyase (PAL) activity第41页
        3.3.3 Protein determination第41-42页
4 RESULTS第42-62页
    4.1 ”IN VITRO” Antagonistic tests of Trichoderma harzianum NF9 against the rice pathogens第42-50页
        4.1.1 Dual culture第42-46页
        4.1.2 The filter culture第46-50页
    4.2 Greenhouse experiments第50-57页
    4.3 Enzyme activity results第57-62页
5 Discussion第62-68页
    5.1 In vitro antagonistic activity of T. harzianum NF9 against the rice pathogens第62-63页
    5.2 The induced resistance of rice by Trichoderma第63-64页
    5.3 Enzyme activity involved in the induced resistance of rice by Trichoderma第64-68页
6 Conclusion and perspectives第68-70页
References第70-84页

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