| Abstract | 第7-8页 |
| Abbreviation | 第9-10页 |
| Chapter one Introduction | 第10-25页 |
| 1.1 Research situation of serine/threonine protein kinase genes | 第10-16页 |
| 1.1.1 Biological function of receptor-like protein kinase (RLK) | 第11-12页 |
| 1.1.2 RLCKs involved in plant immune responses | 第12-14页 |
| 1.1.3 Effect of RLCK on growth and development of plants | 第14页 |
| 1.1.4 Effect of Universal stress protein (USP) in harsh environmental conditions | 第14-16页 |
| 1.2 Research methods of differential proteins | 第16-21页 |
| 1.2.1 Proteomics technology | 第16页 |
| 1.2.2 Proteomics separation techniques | 第16-21页 |
| 1.3 Bioinformatics | 第21-23页 |
| 1.4 The main objective and significance of the presentive research | 第23-25页 |
| Chapter Two Proteomic analysis of serine/threonine kinase gene family in maize | 第25-32页 |
| 2.1 Materials and methods | 第26-27页 |
| 2.1.1 Retrieval of Amino acid Sequences of STK in Maize | 第26页 |
| 2.1.2 Sequence Analysis of STK in Maize | 第26页 |
| 2.1.3 Expansion Mode Analysis of STK Family | 第26页 |
| 2.1.4 Homology Analysis of STK Family with USP Domain | 第26-27页 |
| 2.2 Results and Discussion | 第27-32页 |
| 2.2.1 STKs in Maize Genome | 第27-28页 |
| 2.2.2 Analysis of Motifs in STK Family in Maize | 第28页 |
| 2.2.3 Analysis of Expansion Mode of STK Family Members in Maize | 第28-29页 |
| 2.2.4 Phylogenetic Analysis of Candidate Sequences with USP Domain | 第29-32页 |
| Chapter three The USP domain cloning and expression of receptor-kinase STK2 | 第32-54页 |
| 3.1 Materials | 第32-35页 |
| 3.1.1 Plant materials | 第32页 |
| 3.1.2 Strains and vectors | 第32-33页 |
| 3.1.3 Test reagents | 第33页 |
| 3.1.4 Test instruments | 第33页 |
| 3.1.5 The main solution | 第33-35页 |
| 3.2 Methods | 第35-48页 |
| 3.2.1 The construction of STK2-USP cloning vector | 第35-43页 |
| 3.2.2 Construction of STK2-USP expression vector | 第43-45页 |
| 3.2.3 Prokaryotic expression of His-STK2-USP fusion protein | 第45-48页 |
| 3.3 Result and analysis | 第48-53页 |
| 3.3.1 The construction of STK2-USP cloning vector | 第48-49页 |
| 3.3.2 Construction of STK2-USP prokaryotic expression vector | 第49-51页 |
| 3.3.3 Prokaryotic expression His-STK2-USP fusion protein | 第51-53页 |
| 3.4 Discussion | 第53-54页 |
| Chapter four Differential protein iTRAQ analysis of maize pollen mutants | 第54-79页 |
| 4.1 Materials Methods | 第54-58页 |
| 4.1.1 Maize materials, field planting and sampling | 第54-55页 |
| 4.1.2 Sample preparation and iTRAQ labeling | 第55页 |
| 4.1.3 LC-MS/MS and data analysis | 第55-56页 |
| 4.1.4 Phosphopeptide enrichment, identification and phosphorylation residue localization | 第56-57页 |
| 4.1.5 Bioinformatics | 第57-58页 |
| 4.2 Results and Analysis | 第58-71页 |
| 4.2.1 Mass spectrometric detection results | 第58-65页 |
| 4.2.2 Annotation of differentially expressed proteins in GO | 第65页 |
| 4.2.3 Metabolic pathway analysis of differentially expressed protein | 第65-68页 |
| 4.2.4 Functional classification of differentially expressed proteins | 第68-70页 |
| 4.2.5 The interaction network analysis of differentially expressed proteins | 第70-71页 |
| 4.3 Discussion | 第71-77页 |
| 4.3.1 Total protein mass spectra of maize pollen development stage and differentially expressed proteins of zmstk2 mutants were obtained for the first time | 第71-72页 |
| 4.3.2 Functional annotation analysis | 第72页 |
| 4.3.3 Function of differentially expressed proteins and its influence on pollen development | 第72-76页 |
| 4.3.4 Network metabolic analysis | 第76-77页 |
| 4.4 Conclusions | 第77-79页 |
| References | 第79-88页 |
| Acknowledgements | 第88-89页 |
| Published papers during Ph.D | 第89-90页 |
