| 摘要 | 第5-7页 |
| ABSTRACT | 第7-8页 |
| CHAPTER Ⅰ. GENERAL INTRODUCTION | 第18-23页 |
| 1.1 INTRODUCTION | 第18-19页 |
| 1.2 GENERAL INFORMATION, INSECT PEST AND PATHOGEN, ANDIMPORTANCE ABOUT CHINESE WHITE PINE (P. ARMANDI) | 第19-21页 |
| 1.2.1 General information about Chinese white pine | 第19页 |
| 1.2.2 Insect pest and pathogen on Chinese white pine | 第19-20页 |
| 1.2.3 Importance of Chinese white pine | 第20-21页 |
| 1.3 IMPORTANCE AND RELEVANCE OF THIS STUDY | 第21-22页 |
| 1.4 OBJECTIVE OF THIS STUDY | 第22-23页 |
| CHAPTER Ⅱ. LITERATURE REVIEW 6 | 第23-31页 |
| 2.1 INTRODUCTION | 第23页 |
| 2.2 OVERVIEW OF PLANT CYTOCHROME P450 | 第23-26页 |
| 2.2.1 Detection of cytochrome P450 | 第23-24页 |
| 2.2.2 Cytochrome P450 taxonomic system | 第24页 |
| 2.2.3 Research of cytochromes P450 genes in plants | 第24-26页 |
| 2.3 OVERVIEW TERPENOIDS OF PLANTS | 第26-31页 |
| 2.3.1 Plant terpenoids biosynthesis | 第26-27页 |
| 2.3.2 The major plant terpenoid compounds | 第27-28页 |
| 2.3.3 Research on terpenoids in conifer | 第28-31页 |
| CHAPTER Ⅲ. ISOLATION A P450 GENE IN PINUS ARMANDI AND ITSEXPRESSION AFTER INOCULATION OF LEPTOGRAPHIUM QINLINGENSISAND TREATMENT WITH METHYL JASMONATE | 第31-50页 |
| ABSTRACT | 第31-32页 |
| 3.1 INTRODUCTION | 第32-33页 |
| 3.2 MATERIALS AND METHODS | 第33-42页 |
| 3.2.1 Plant material | 第33-34页 |
| 3.2.2 Amplification of genes, cloning and sequence analyses | 第34-35页 |
| 3.2.3 End sequence determination and cloning of full-length cDNAs | 第35-36页 |
| 3.2.4 Seedling treatment for RT-qPCR | 第36-38页 |
| 3.2.4.1 MeJa treatment | 第36页 |
| 3.2.4.2 L. qinlingensis treatment | 第36-38页 |
| 3.2.5 Tissue harvest, extraction of RNA, selection of primers for RT-qPCR | 第38页 |
| 3.2.6 RT-qPCR | 第38-41页 |
| 3.2.7 Statistical analysis | 第41-42页 |
| 3.3 RESULTS | 第42-46页 |
| 3.3.1 Sequence analysis | 第42-43页 |
| 3.3.2 Gene expression | 第43-46页 |
| 3.4 DISCUSSION | 第46-48页 |
| 3.5 CONCLUSION | 第48-50页 |
| CHAPTER Ⅳ. THE DIFFERENTIAL EFFECTS OF THE BLUE-STAIN FUNGUSLEPTOGRAPHIUM QINLINGENSIS ON MONOTERPENES ANDSESQUITERPENES IN THE STEM OF CHINESE WHITE PINE(PINUS ARMANDI)SAPLINGS | 第50-76页 |
| ABSTRACT | 第50-51页 |
| 4.1 INTRODUCTION | 第51-53页 |
| 4.2 MATERIALS AND METHODS | 第53-57页 |
| 4.2.1 Plant material | 第53页 |
| 4.2.2 Culture of L. qinlingensis | 第53页 |
| 4.2.3 L. qinlingensis treatments | 第53-54页 |
| 4.2.4 Tissue harvesting | 第54-55页 |
| 4.2.5 Analysis of monoterpene and sesquiterpenes | 第55页 |
| 4.2.6 Statistical analysis | 第55-57页 |
| 4.3 RESULTS | 第57-70页 |
| 4.3.1 Effect of L. qinlingensis and mechanical wounding on monoterpenes in the xylem of the stem of P. armandi saplings | 第57-60页 |
| 4.3.2 Effect of L. qinlingensis and mechanical wounding on monoterpenes in phloem of stem in P. armandi saplings | 第60-61页 |
| 4.3.3 Differential effects of L. qinlingensis and mechanical wounding on monoterpenes between phloem and xylem of stem in P. armandi sapling | 第61-68页 |
| 4.3.4 Effect of L. qinlingensis and mechanically wounding on sesquiterpenes in the xylem of the stem in P. armandi saplings | 第68-69页 |
| 4.3.5 Effect of L. qinlingensis and mechanical wounding on sesquiterpenes in phloem of stem in P. armandi saplings | 第69-70页 |
| 4.3.6 Differential effects of L. qinlingensis and mechanical wounding on sesquiterpenes between phloem and xylem of stem in P. armandi saplings | 第70页 |
| 4.4 DISCUSSION | 第70-74页 |
| 4.5 CONCLUSIONS | 第74-75页 |
| ACKNOWLEDGMENTS | 第75-76页 |
| CHAPTER V. CHANGES OF MONOTERPENES IN STEM OF CHINESE WHITEPINE(PINUS ARMANDI) SAPLINGS FOLLOWING TREATMENT WITHMETHYL JASMONATE | 第76-96页 |
| ABSTRACT | 第76-77页 |
| 5.1 INTRODUCTION | 第77-79页 |
| 5.2 MATERIALS AND METHODS | 第79-85页 |
| 5.2.1 Plant material | 第79-80页 |
| 5.2.2 MeJa treatment | 第80页 |
| 5.2.3 Tissue harvesting | 第80-81页 |
| 5.2.4 Analysis of monoterpenes | 第81-84页 |
| 5.2.5 Statistical analysis | 第84-85页 |
| 5.3 RESULTS | 第85-92页 |
| 5.3.1 Effect of MeJa and Tween-20 on monoterpenes in the stem xylem in P. armandi saplings | 第85-88页 |
| 5.3.2 Effect of MeJa and Tween-20 treatment on monoterpenes in stem phloem in P. armandi saplings | 第88-91页 |
| 5.3.3 Differential effect MeJa and Tween-20 on monoterpenes between phloem and xylem in the stem in P. armandi saplings | 第91-92页 |
| 5.4 DISCUSSION | 第92-93页 |
| 5.5 CONCLUSIONS | 第93-95页 |
| ACKNOWLEDMENTS | 第95-96页 |
| REFERENCES | 第96-114页 |
| APPENDIX | 第114-119页 |
| ACKNOWLEDGEMENTS | 第119-121页 |
| AUTHOR'S BRIEF INTRODUCTION | 第121-122页 |
