| 摘要 | 第1-8页 |
| Abstract | 第8-10页 |
| Chapter 1 Introduction | 第10-30页 |
| 1.Introduction of self-assemble monolayers | 第10-17页 |
| ·Introduction | 第10-12页 |
| ·Fabrication and mechanism of forming SAMs on gold substrate | 第12-15页 |
| ·Applications of self-assembled monolayers | 第15-17页 |
| 2.Importance of this research | 第17-19页 |
| ·Surface pK_a of SAMs | 第17-18页 |
| ·Electrochemical behavior of epinephrine at different SAMs | 第18-19页 |
| ·Kinetic analysis of immunoreaction on modified electrode | 第19页 |
| 3.Prospective | 第19-20页 |
| 4.References | 第20-30页 |
| Chapter 2 Determination of the surface isoelectric point of self-assembled molecular films on gold by voltammetry titration | 第30-39页 |
| 1.Introduction | 第30-31页 |
| 2.Experimental | 第31-32页 |
| ·Reagents and materials | 第31页 |
| ·Apparatus | 第31页 |
| ·Preparation of SAMs electrodes | 第31-32页 |
| 3.Results and discussion | 第32-36页 |
| ·Charge-transfer of Fe(CN)_6~(3-/4-) at gold electrode modified by 1,3-benzenedithiol | 第32页 |
| ·Determination of surface pK of SAM-modified Au electrode with acidic or basic groups | 第32-34页 |
| ·Determination of surface isoelectric point at gold electrode modified by L-Cys and PCA | 第34-36页 |
| 4.Conclusions | 第36-37页 |
| 5.References | 第37-39页 |
| Chapter 3 Electrochemical behavior of epinephrine at different self-assembled mouolayer on gold electrode | 第39-56页 |
| 1.Introduction | 第39页 |
| 2.Experimental | 第39-40页 |
| ·Reagents | 第39-40页 |
| ·Apparatus | 第40页 |
| ·Preparation of SAMs | 第40页 |
| 3.Research of EP at two-component self-assembled monolayer of meso-2,3-dimercaptosuccinic acid and penicillamine | 第40-47页 |
| ·Characterization of mixed SAM in a Fe(CN)_6~(3-)/Fe(CN)_6~(4-) solution | 第41页 |
| ·Characterization for the DMSA and PCA mixed (2:1) SAM by electrochemical impedance spectroscopy | 第41-42页 |
| ·Electrochemical response of EP at the mixed SAM electrodes | 第42-43页 |
| ·Effects of pH on the peak current and peak potential of EP | 第43-44页 |
| ·Effect of scan rate on the peak current | 第44页 |
| ·Determination of the electrochemical parameters | 第44-45页 |
| ·Stability and standard deviation of the mixed SAM electrode | 第45页 |
| ·Calibration plots | 第45-46页 |
| ·Tolerance of foreign substances | 第46-47页 |
| ·Analytical application | 第47页 |
| 4.Research of EP at DTDPA/Au electrode | 第47-53页 |
| ·Characterization of the DTDPA/Au SAM in a Fe(CN)_6~(3-)/Fe(CN)_6~(4-) solution | 第47-48页 |
| ·Characterization for the DTDPA/Au SAM by AC impedance spectroscopy | 第48-49页 |
| ·Electrochemical response of the DTDPA/Au sAM on the oxidation of EP | 第49-50页 |
| ·Effect of pH value | 第50页 |
| ·Effect of the scan rate on the peak current | 第50-51页 |
| ·Stability of the DTDPA SAM electrode | 第51-52页 |
| ·Calibration plots | 第52-53页 |
| ·Tolerance of foreign substances | 第53页 |
| ·Analytical application | 第53页 |
| 5.References | 第53-56页 |
| Chapter 4 Thermodynamic and kinetic analysis of the interaction between hepatitis B surface antibody and antigen on SAMs electrode | 第56-77页 |
| 1.Introduction | 第56-57页 |
| 2.Experimental | 第57-58页 |
| ·Materials | 第57页 |
| ·Apparatus | 第57页 |
| ·Preparation and characterization of colloidal gold | 第57-58页 |
| ·Electrochemical measurement | 第58页 |
| 3.Research on a gold electrode modified with cysteamine and colloidal gold via DPV | 第58-67页 |
| ·Antibody immobilization | 第59页 |
| ·Cyclic voltammetry characteristics on the modified electrode surface | 第59-61页 |
| ·Impedance characteristics of antibody-antigen binding | 第61-62页 |
| ·Effect of the incubation time of HBsAb modified electrode on DPV response of the HBsAg solution | 第62-63页 |
| ·Effect of the incubation temperature of HBsAb modified electrode on DPV response of the HBsAg solution | 第63页 |
| ·Kinetic analysis for the affinity binding of HBsAg | 第63-66页 |
| ·Thermodynamic analysis for the affinity binding of HBsAg | 第66-67页 |
| 4.Research on a gold electrode modified with 1,6-hexanedithiol and colloidal gold via EIS | 第67-74页 |
| ·Antibody immobilization | 第67页 |
| ·Characteristics of modified monolayer | 第67-70页 |
| ·Theoretical impedance | 第70页 |
| ·Effect of the incubation time of HBsAb modified electrode on the HBsAg solution | 第70-71页 |
| ·Effect of the incubation temperature of HBsAb modified electrode | 第71-72页 |
| ·Kinetic analysis for the affinity binding of HBsAg | 第72-74页 |
| 5.Contrastive analysis of the affinity binding of HBsAg | 第74-75页 |
| 6.Conclusions | 第75页 |
| 7.References | 第75-77页 |
| Conclusions | 第77-78页 |
| 致谢 | 第78-79页 |
| Appendix:The papers published during the graduate | 第79页 |
