| Abstract | 第1-10页 |
| 摘要 | 第10-13页 |
| Table of Contents | 第13-15页 |
| 1 Introduction | 第15-44页 |
| ·SARS epidemiology and etiology | 第15-17页 |
| ·Origin of SARS virus | 第17-18页 |
| ·SARS-CoV genome structure | 第18-22页 |
| ·SARS-CoV structural proteins | 第22-24页 |
| ·SARS-CoV nucleocapsid protein | 第24-26页 |
| ·The first research objective: SARS-CoV N protein used for serodiagnosis | 第26-28页 |
| ·The second research objective: sub-cellular localization properties of SARS-CoVN protein | 第28-38页 |
| ·The third research objective: investigate the possible role of N protein in signal transduction pathway | 第38-44页 |
| 2 Materials and method | 第44-56页 |
| ·Clinical Samples | 第44页 |
| ·Cells culture | 第44-45页 |
| ·Virus infection and isolation of viral RNA | 第45页 |
| ·Reverse transcription-polymerase chain reaction (RT-PCR) | 第45-46页 |
| ·Sequence analysis of SARS-CoV N protein gene | 第46页 |
| ·Plasmids construction | 第46-49页 |
| ·Prokaryotic expression and purification of SARS-CoV N protein | 第49-50页 |
| ·Preparing microplates for Enzyme-Linked Immunosorbent Assay | 第50-51页 |
| ·Generation of anti-N polyclonal antibody in rabbit | 第51页 |
| ·Cell transfection | 第51-52页 |
| ·Establishments of stable nucleocapsid protein-expressing Vero E6 cell line | 第52页 |
| ·Western blotting | 第52-53页 |
| ·Indirect immunofiuorescence assay and confocal microscopy | 第53页 |
| ·LeptomycinB treatment | 第53页 |
| ·Flow cytometry and colony formation efficiency assays | 第53-54页 |
| ·Luciferase assay | 第54-56页 |
| 3 Results | 第56-88页 |
| ·SARS-CoV nucleocapsid protein gene sequence analysis | 第56-61页 |
| ·Expression and characterization of SARS-CoV recombinant nucleocapsid protein | 第61-62页 |
| ·Detection of IgG antibodies against N protein in the sera of SARS patients | 第62-65页 |
| ·Production and characterization of rabbit anti-nucleocapsid polyclonal antibodies | 第65-68页 |
| ·Intracellular localization of SARS-CoV N protein in infected and transfected cell lines | 第68-71页 |
| ·Subcellular localization of N protein alters upon N- and/or C-terminal deletion mutations | 第71-74页 |
| ·Expression and intracellular localization of N-GFP fusion proteins | 第74-77页 |
| ·The leucine rich region may act as nuclear export signal | 第77-80页 |
| ·Stable cell line constitutively expressing N protein affects cell cycle profile | 第80-83页 |
| ·SARS-CoV N protein can activates NF-kB in dose-dependent manner | 第83-86页 |
| ·The conformation of SARS-CoV N protein is important for its NF-kB activation | 第86-88页 |
| 4 Discussion | 第88-115页 |
| 5 References | 第115-147页 |
| 6 Acknowledgments | 第147-149页 |
| 7 List of Figures | 第149-151页 |
| 8 List of Tables | 第151-152页 |
| 9 Publications | 第152页 |
