| Abstract | 第5页 |
| Introduction | 第6-23页 |
| 1 Plant Endophytic Bacteria | 第6-12页 |
| 1.1 Conception of plant endophytic bacteria | 第6-8页 |
| 1.2 Colonization of plant endophytic bacteria | 第8-12页 |
| 1.2.1 Methods for detecting the bacterial colonization | 第8-10页 |
| 1.2.2 Factors of influenceing the bacterial colonization | 第10-12页 |
| 2 Associative Nitrogen-fixing Bacteria | 第12-19页 |
| 2.1 Species of the associative nitrogen-fixing bacteria | 第12-14页 |
| 2.2 Function of the associative nitrogen-fixing bacteria | 第14-17页 |
| 2.3 Growth conditions of the associative nitrogen-fixing bacteria | 第17-19页 |
| 3 Associative Nitrogen-fixing Bacteria on and in Rice Plants | 第19-21页 |
| 3.1 Associative nitrogen-fixing bacteria in rice rhizosphere | 第19-20页 |
| 3.2 Endophytic associative nitrogen-fixing bacteria in rice plants | 第20-21页 |
| 4 Goal and Significance of this research | 第21-23页 |
| Materials and Methods | 第23-26页 |
| 1 Bacterial Isolates | 第23页 |
| 2 Tests on the Growth Conditions of the Nitrogen-fixing Bacteria | 第23-24页 |
| 2.1 Making a standard curve-bacterial concentration (cfu/ml) vs absorbance | 第23页 |
| 2.2 Method for detecting the effects of culture time on the bacterial growth | 第23-24页 |
| 2.3 Method for detecting the effects of temperature on the bacterial growth | 第24页 |
| 2.4 Method for detecting the effects of medial pH on the bacterial growth | 第24页 |
| 2.5 Method for detecting the effects of vibration culture on the bacterial growth | 第24页 |
| 3 Detection of the Colonization of Rice Endophytic Nitrogen-fixing Bacteria | 第24-26页 |
| 3.1 Method for the induction of the bacterial strains resistant to rifampicin | 第24页 |
| 3.2 Detection of the bacterial colonization on or in rice leaves after spray | 第24-25页 |
| 3.3 Detection of the bacterial colonization in rice plants after dipping roots | 第25-26页 |
| Results and Analyses | 第26-32页 |
| 1 Growth Conditions of Rice Endophytic Nitrogen-fixing Bacteria | 第26-29页 |
| 1.1 Standard curve-bacterial concentration (cfu/ml) vs absorbance | 第26-27页 |
| 1.2 Effects of culture time on the bacterial growth | 第27页 |
| 1.3 Effects of culture temperature on the bacterial growth | 第27-28页 |
| 1.4 Effects of medial pH on the bacterial growth | 第28页 |
| 1.5 Effects of vibration culture on the bacterial growth | 第28-29页 |
| 2 Colonization of the Nitrogen-fixing Bacteria in and on Rice Plants | 第29-32页 |
| 2.1 Induction of rifampicin resistant strains from the nitrogen-fixing bacteria | 第29页 |
| 2.2 Colonization of the nitrogen-fixing bacteria on and in rice plants after spray | 第29-30页 |
| 2.3 Colonization of the nitrogen-fixing bacteria in rice plants after root-dipped | 第30-32页 |
| Conclusion and Discussion | 第32-34页 |
| 1 Conclusion | 第32页 |
| 2 Discussion | 第32-34页 |
| 2.1 Growth conditions of rice endophytic nitrogen-fixing bacteria | 第32页 |
| 2.2 Colonization of the nitrogen-fixing bacteria in and on rice plants | 第32-33页 |
| 2.3 Prospects of rice endophytic nitrogen-fixing bacteria applied in agriculture | 第33-34页 |
| Reference | 第34-43页 |
| Acknowledgement | 第43页 |
