Abstract English | 第5-6页 |
Abstract Chinese | 第7-11页 |
List of abbreviations | 第11-12页 |
Chapter 1:Introduction | 第12-14页 |
1.1 Preface | 第12-13页 |
1.2 Motivation | 第13-14页 |
Chapter 2:Materials and Methods | 第14-21页 |
2.1 Materials | 第14页 |
2.2 Identification methods | 第14-17页 |
2.2.1 Rice growing and fungus infection | 第14页 |
2.2.2 Leaf total RNA extraction and RNA-Seq | 第14-16页 |
2.2.3 Sequencing quality controlling | 第16页 |
2.2.4 Detection of differentially Expressed Genes | 第16-17页 |
2.3 Functional characterizations of Differentially Expressed Genes | 第17-19页 |
2.3.1 Gene Ontology analysis | 第17页 |
2.3.2 Metabolic pathways analysis | 第17-18页 |
2.3.3 Analysis of protein families | 第18-19页 |
2.4 Co-expression network analysis | 第19-20页 |
2.5 Phylogenetic inference | 第20-21页 |
Chapter 3:Results and Analysis | 第21-53页 |
3.1 The quality of RNA-Seq Reads | 第21-24页 |
3.1.1 Base composition of sequenced reads | 第21-23页 |
3.1.2 Transcriptome coverage | 第23-24页 |
3.2 Identification of blast responsive genes | 第24-33页 |
3.2.1 DEGs in the 5 NILs at 24hpi compared to CO39 | 第24页 |
3.2.2 DEGs in the 5 NILs at 48hpi compared to CO39 | 第24-26页 |
3.2.3 DEGs at 24hpi with water treatment as control | 第26-27页 |
3.2.4 DEGs 48hpi with water treatment as control sample | 第27-30页 |
3.2.5 Isoforms in the 5 NILs under 24hpi compared to CO39 | 第30-31页 |
3.2.6 Significantly regulated genes in the 5 NILs at 24hpi compared to CO39 | 第31-33页 |
3.3 Functional characterizations of Diferentially Expressed Genes | 第33-50页 |
3.3.1 Gene ontologies | 第33-42页 |
3.3.2 Metabolic pathways | 第42-43页 |
3.3.3 Protein families | 第43-50页 |
3.4 Co-expression network | 第50-52页 |
3.5 Phylogenetic profiling | 第52-53页 |
Chapter 4:Discussion | 第53-59页 |
4.1 DEGs induced by blast-fungus in rice cultivars | 第53-55页 |
4.2 Functional analysis of DEGs induced by blast fungus in rice | 第55-56页 |
4.3 Protein family members iduced by GUY11 in rice | 第56-59页 |
Chapter 5:Conclusion | 第59-61页 |
References | 第61-65页 |
Supplementary materials | 第65-71页 |
Acknowledgment | 第71页 |