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水稻CO39品种及其5个近等基因系对稻瘟病感染应答的转录组分析

Abstract English第5-6页
Abstract Chinese第7-11页
List of abbreviations第11-12页
Chapter 1:Introduction第12-14页
    1.1 Preface第12-13页
    1.2 Motivation第13-14页
Chapter 2:Materials and Methods第14-21页
    2.1 Materials第14页
    2.2 Identification methods第14-17页
        2.2.1 Rice growing and fungus infection第14页
        2.2.2 Leaf total RNA extraction and RNA-Seq第14-16页
        2.2.3 Sequencing quality controlling第16页
        2.2.4 Detection of differentially Expressed Genes第16-17页
    2.3 Functional characterizations of Differentially Expressed Genes第17-19页
        2.3.1 Gene Ontology analysis第17页
        2.3.2 Metabolic pathways analysis第17-18页
        2.3.3 Analysis of protein families第18-19页
    2.4 Co-expression network analysis第19-20页
    2.5 Phylogenetic inference第20-21页
Chapter 3:Results and Analysis第21-53页
    3.1 The quality of RNA-Seq Reads第21-24页
        3.1.1 Base composition of sequenced reads第21-23页
        3.1.2 Transcriptome coverage第23-24页
    3.2 Identification of blast responsive genes第24-33页
        3.2.1 DEGs in the 5 NILs at 24hpi compared to CO39第24页
        3.2.2 DEGs in the 5 NILs at 48hpi compared to CO39第24-26页
        3.2.3 DEGs at 24hpi with water treatment as control第26-27页
        3.2.4 DEGs 48hpi with water treatment as control sample第27-30页
        3.2.5 Isoforms in the 5 NILs under 24hpi compared to CO39第30-31页
        3.2.6 Significantly regulated genes in the 5 NILs at 24hpi compared to CO39第31-33页
    3.3 Functional characterizations of Diferentially Expressed Genes第33-50页
        3.3.1 Gene ontologies第33-42页
        3.3.2 Metabolic pathways第42-43页
        3.3.3 Protein families第43-50页
    3.4 Co-expression network第50-52页
    3.5 Phylogenetic profiling第52-53页
Chapter 4:Discussion第53-59页
    4.1 DEGs induced by blast-fungus in rice cultivars第53-55页
    4.2 Functional analysis of DEGs induced by blast fungus in rice第55-56页
    4.3 Protein family members iduced by GUY11 in rice第56-59页
Chapter 5:Conclusion第59-61页
References第61-65页
Supplementary materials第65-71页
Acknowledgment第71页

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