| 摘要 | 第1-5页 |
| ABSTRACT | 第5-8页 |
| LIST OF ABBREVATIONS | 第8-9页 |
| LIST OF TABLES | 第9-10页 |
| LIST OF FIGURES | 第10-13页 |
| CHAPTER 1 GENERAL INTRODUCTION | 第13-26页 |
| INTRODUCTION | 第13-14页 |
| REVIEW OF LITERATURE | 第14页 |
| Head smut of maize | 第14-16页 |
| Head smut management | 第16-17页 |
| Head smut resistance gene in maize | 第17-20页 |
| Correlation between disease resistance gene and agronomic traits | 第20-23页 |
| Detection and quantification of pathogen biomass in plant | 第23-25页 |
| OBJECTIVES | 第25-26页 |
| CHAPTER 2 MATERIALS AND METHODS | 第26-33页 |
| Plant Materials | 第26页 |
| Preparation of inocula | 第26页 |
| Field design and inoculation | 第26-27页 |
| Evaluation of yield and agronomic traits in hybrids | 第27-28页 |
| Sampling for detection and estimation of the amount of S.reiliana DNA | 第28页 |
| Extraction of genomic DNA of maize and S.reiliana | 第28-30页 |
| Detection of S.reiliana DNA in plant material with the conventional PCR | 第30页 |
| Detection and estimation of the amount of S.reiliana DNA in plant material with the real-time PCR | 第30页 |
| Construction of the standard calibration curve | 第30-31页 |
| Determination of total sugar from maize seedling | 第31页 |
| Statistical analyses | 第31-33页 |
| CHAPTER 3 RESULTS | 第33-60页 |
| ·Resistance and agronomic performance converted inbred lines and hybrids under disease conditions | 第33-44页 |
| ·Resistance performance of converted inbred lines and hybrids | 第33-39页 |
| ·Impact of ZmWAK on yield per plot | 第39-40页 |
| ·Genetic effect of ZmWAK on yield-related traits | 第40-44页 |
| ·Quantification of S. reiliana DNA content in symptomless plant | 第44-52页 |
| ·Specificity of primers for Real-time PCR | 第44-46页 |
| ·Optimization of DNA template concentration for real-time PCR | 第46-47页 |
| ·Real-time PCR standard calibration curve | 第47-49页 |
| ·Infection rate | 第49-50页 |
| ·Estimation of the quantity of endophytic S. reiliana DNA in symptomless plant | 第50-51页 |
| ·Correlation of the quantity of S. reiliana DNA with yield-related traits | 第51-52页 |
| ·Changes of total sugar in mesocotyl | 第52页 |
| ·Agronomic performances of hybrids before and after introgression of ZmWAK underdisease-free condition | 第52-60页 |
| CHAPTER 4 DISCUSSION | 第60-67页 |
| Correlation between head smut resistance and yield performance | 第60-61页 |
| Effect of ZmWAK on yield-related traits | 第61-62页 |
| Detection of head smut S. reiliana DNA in symptomless plant | 第62-63页 |
| ZmWAK indirectly benefits yield-related traits by reducing the endophytic S. reiliana within maize plant | 第63-65页 |
| Agronomic performance of hybrids before and after introgression of ZmWAK under disease-free condition | 第65-67页 |
| CHAPTER 5 GENERAL CONCLUSIONS | 第67-68页 |
| REFERENCES | 第68-77页 |
| ACKNOWLEDGEMENTS | 第77-78页 |
| CURRICULUM VITAE | 第78页 |
