| ABSTRACT | 第1-8页 |
| 摘要 | 第8-12页 |
| Chapter I General Introduction | 第12-16页 |
| Chapter II Literature review | 第16-41页 |
| ·Use of pesticides and Environmental risk | 第16-18页 |
| ·Potential sources of pesticide residues in food and environment | 第18-20页 |
| ·Application of pesticides to protect the growing crop | 第19页 |
| ·Contamination of the environment by highly stable pesticides | 第19-20页 |
| ·Application of pesticide to protect the harvested crop during storage and handling | 第20页 |
| ·Pesticide fate and dissipation study | 第20-25页 |
| ·Pesticides dissipation fate on plant surfaces and in plants | 第20-22页 |
| ·Nature of pesticide residues in plant and environment | 第22-23页 |
| ·Pesticide residue data in some agricultural products and water | 第23-25页 |
| ·Field dissipation of pesticide residues | 第25-26页 |
| ·Procedures used for determination of pesticides in/on matrix extract | 第26-37页 |
| ·Sampling | 第26页 |
| ·Sample preparation | 第26-35页 |
| ·Sample analysis (Instrumental analysis) | 第35-37页 |
| ·Method Validation | 第37页 |
| ·Propamidine fungicide | 第37-41页 |
| ·Historic | 第37-38页 |
| ·Multiple effects of propamidine | 第38页 |
| ·Physical and chemical properties | 第38-39页 |
| ·Formulations and appearance | 第39页 |
| ·Eco-toxicity of propamidine fungicide | 第39页 |
| ·Application area of propamidine fungicide | 第39-41页 |
| Chapter III Method Determination and Validation | 第41-61页 |
| 3 Determination of the analytical method and Validation | 第41-61页 |
| ·Material | 第41-42页 |
| ·Solvents, reagents and Pesticide | 第41页 |
| ·Instrumental | 第41-42页 |
| ·Preparations of Standard Solutions | 第42页 |
| ·Preparation of Phosphate Buffered Saline (PBS) solution | 第42页 |
| ·Procedure of propamidine-fungicide analysis in tomato fortified sample | 第42-45页 |
| ·Sample preparation | 第42-43页 |
| ·Sample extraction and analysis | 第43-44页 |
| ·Quantitation | 第44-45页 |
| ·Validation of the method | 第45-47页 |
| ·Calibration curve and linearity | 第45页 |
| ·Selectivity and stability of propamidine fungicide in solvent and tomato extract | 第45页 |
| ·Recovery and Accuracy | 第45-46页 |
| ·Repeatability Precision and matrix effect | 第46-47页 |
| ·Limits of Detection and Quantification | 第47页 |
| ·Statistical analysis | 第47页 |
| ·Results of method determination and validation | 第47-61页 |
| ·Method determination | 第47-54页 |
| ·Determination of Chromatographic conditions | 第47-48页 |
| ·Chromatographic peak identification | 第48页 |
| ·Determination of Calibration Plot for method determination | 第48-50页 |
| ·Linearity and Range | 第50页 |
| ·Recovery efficiency of propamidine fungicide from fortified tomato sample | 第50-53页 |
| ·Experimental Conclusion | 第53-54页 |
| ·Method validation | 第54-61页 |
| ·Analytical curve and linearity | 第54-55页 |
| ·Quantification | 第55页 |
| ·Selectivity and Stability of propamidine fungicide in solvent and tomato extract | 第55-57页 |
| ·Repeatability of injection (short-term precision) and matrix effect | 第57-58页 |
| ·Accuracy and recovery | 第58-59页 |
| ·Limits of Detection and Quantification | 第59页 |
| ·Experimental conclusion | 第59-61页 |
| Chapter IV Laboratory Experimentation | 第61-72页 |
| 4 Propamidine fungicide residue dynamic study in tomato in glasshouse condition | 第61-72页 |
| ·Materiel and method | 第61-63页 |
| ·Pesticide | 第61页 |
| ·Glasshouse | 第61页 |
| ·Vegetal materiel | 第61页 |
| ·Experimental design and trial implantation | 第61-62页 |
| ·Application of pesticide | 第62-63页 |
| ·Sample collection of and storage | 第63页 |
| ·Evaluation of the influence of the additional insecticide on propamidine fungicide detection | 第63-64页 |
| ·Propamidine fungicide residues dissipation in tomatoes | 第64页 |
| ·Results of laboratory study | 第64-72页 |
| ·Evaluation of influence of imidacloprid on propamidine fungicide detection | 第64-66页 |
| ·Propamidine fungicide residues dissipation in laboratory glasshouse condition | 第66-72页 |
| ·Propamidine Residue Levels in Fresh Harvested Tomato (terminal residues) | 第66-69页 |
| ·Dissipation of propamidine in tomato | 第69-71页 |
| ·Experimental Conclusion | 第71-72页 |
| Chapter V Field Experimentation | 第72-82页 |
| 5 Propamidine fungicide residue dynamic study in tomato in greenhouse condition | 第72-82页 |
| ·Materiel and method | 第72-74页 |
| ·Solvents, Reagent and Pesticide | 第72页 |
| ·Vegetal materiel | 第72页 |
| ·Experimental design | 第72-73页 |
| ·Sampling and Storage | 第73-74页 |
| ·Propamidine fungicide residues dissipation | 第74页 |
| ·Results of propamidine residues dissipation study in tomato in greenhouse condition | 第74-78页 |
| ·Propamidine Residue Levels in Fresh Harvested Tomato (terminal residues) | 第74-76页 |
| ·Dissipation of propamidine in tomato | 第76-78页 |
| ·Experimental conclusions | 第78页 |
| ·Comparison and synthesis of results obtained from glasshouse and solargreenhouse | 第78-82页 |
| Chapter VI Summary, Conclusion and Recommendations | 第82-86页 |
| Summary | 第82-83页 |
| Conclusion | 第83-85页 |
| Recommendations | 第85-86页 |
| Acknowledgements | 第86-87页 |
| Reference | 第87-97页 |
| Appendixes | 第97-101页 |
| List of Acronyms and Abbreviations | 第101-103页 |
| List of Table | 第103-104页 |
| List of figures | 第104页 |
