| Abstract | 第1-8页 |
| Chapter 1. Aloe Emodin-Induced Apoptosis in t-HSC/CI-6 Cells Involves a Mitochondria-Mediated Pathway | 第8-25页 |
| 1. Introduction | 第9-11页 |
| 2. Materials and Methods | 第11-17页 |
| ·Chemical reagents | 第11页 |
| ·Cell culture | 第11页 |
| ·Cell viability assay | 第11-12页 |
| ·Observation of morphology changes | 第12页 |
| ·DNA fragmentation analysis | 第12页 |
| ·Flow cytometry analysis | 第12-13页 |
| ·Measurement of mitochondrial membrane potential | 第13-14页 |
| ·Assay of caspase activity | 第14页 |
| ·Preparation of whole cell lysates | 第14-15页 |
| ·Isolation of cytosol and mitochondrial extract | 第15页 |
| ·Western blot analysis | 第15-16页 |
| ·Statistical analysis | 第16-17页 |
| 3. Results | 第17-22页 |
| ·Growth inhibition | 第17页 |
| ·Effect of AE apoptosis in t-HSC/CI-6 cells | 第17-18页 |
| ·Caspase activation in AE-induced apoptosis | 第18-20页 |
| ·Expression of Bax and Bcl-2 proteins induced by AE treatment | 第20-21页 |
| ·Involvement of a mitochondrial pathway in AE-induced apoptosis | 第21-22页 |
| 4. Discussion | 第22-25页 |
| Chapter 2. Cryptotanshinone Induces Apoptosis in t-HSC7CI-6 Cells and salvia miltionhiza Inhibits Hepatic Fibrosis Induced by Carbon Tetrachloride | 第25-47页 |
| 1. Introduction | 第26-29页 |
| 2. Materials and Methods | 第29-35页 |
| ·Chemical reagents | 第29页 |
| ·Preparation of salvia miltiorrhiza standard fraction | 第29页 |
| ·Cell culture | 第29-30页 |
| ·Cell growth assay | 第30页 |
| ·DNA fragmentation analysis | 第30页 |
| ·Now cytometry analysis of annexin v-FTTC binding | 第30-31页 |
| ·Caspase activity assay | 第31页 |
| ·Cytosol and mitochondrial fraction isolation | 第31页 |
| ·Western blot analysis | 第31页 |
| ·Animals | 第31-32页 |
| ·salvia miltiorrhiza standard fraction pre-treatment in CCl_4-induced acute liver injury in rats | 第32页 |
| ·salvia milliorrhiza standard fraction pre-treatment in CCl_4-induced subacute liver injury in rats | 第32-33页 |
| ·Determination of serum biochemical parameters | 第33页 |
| ·Determination of lipid peroxidation levels in liver | 第33页 |
| ·Hislological and immunohistochemistry examination | 第33-34页 |
| ·Statistical analysis | 第34-35页 |
| 3. Results | 第35-41页 |
| ·Anti-proliferative effects of salvia miltiorrhiza standard fraction tanshinone I. tanshinone HA and cryptotanshinone | 第35页 |
| ·Occurrence of apoptosis induced by cryptotanshinone | 第35-36页 |
| ·Cryptotanshinone-induced caspase-3. -9 activation. PARP cleavage and cytochromee release | 第36-38页 |
| ·Cryptotanshinone induced the cleavage of Bax residing exclusively in the mitochondria | 第38页 |
| ·Cryptotanshinone-induced apoptosis is independent of the antiapoptotie protein Bcl-2 | 第38-39页 |
| ·Protective effective of salvia milliorrhiza standard fraction pre-treatment in CCl_4-induced acute liver injury in vivo | 第39页 |
| ·Protective effective of salvia miltiorrhiza standard fraction pre-treatmenti CCl_4-induced subacute liver injury in vivo | 第39-41页 |
| 4.Discussion | 第41-47页 |
| Conclusions | 第47-48页 |
| References | 第48-54页 |
| Figure legends | 第54-58页 |
| Table | 第58-60页 |
| Figure | 第60-72页 |
| Review | 第72-85页 |
| Chinese Appendix | 第85-88页 |
| Abstract | 第86-88页 |
| Review | 第88-97页 |
| Acknowledgement | 第97页 |
