| 摘要 | 第3-7页 |
| Abstract | 第7-9页 |
| Chapter 1 Literature Review | 第13-35页 |
| Introduction | 第13页 |
| 1.1 Classification of HPLC | 第13-23页 |
| 1.1.1 Single-mode chromatography | 第14-17页 |
| 1.1.2 Mixed-mode chromatographic stationary phase | 第17-23页 |
| 1.2 Retention mechanism of HPLC | 第23-27页 |
| 1.2.1 Retention mechanism of single-mode chromatography | 第23-25页 |
| 1.2.2 Retention mechanism of mixed-mode chromatography | 第25-27页 |
| 1.3 Amino-functionalized stationary phase | 第27-32页 |
| 1.3.1 Preparation methods of APS | 第28-29页 |
| 1.3.2 Research status of amino-functionalized stationary phase | 第29-32页 |
| 1.4 Research basis, objectives, design thoughts and contents | 第32-35页 |
| Chapter 2 Experimental | 第35-49页 |
| 2.1 Reagents and instruments | 第35-38页 |
| 2.1.1 Reagents | 第35-37页 |
| 2.1.2 Materials and instruments | 第37-38页 |
| 2.2 Preparation of aminopropyl-functionalized stationary phase, APS | 第38-41页 |
| 2.2.1 Preparation of APS by toluene method | 第38-39页 |
| 2.2.2 Preparation of APS by chemical vapor deposition method | 第39页 |
| 2.2.3 Preparation of APS by impregnation method | 第39-40页 |
| 2.2.4 Characterization of APS stationary phase | 第40-41页 |
| 2.2.5 Chromatographic evaluation of APS stationary phase | 第41页 |
| 2.3 Preparation of ethane-bridged pre-bonded amino-functionalized stationaryphase | 第41-45页 |
| 2.3.1 Preparation of ethane-bridged pre-bonded amino-functionalized(BTME-H-APS) stationary phase | 第41-42页 |
| 2.3.2 BTME-H-APS preparing conditions study | 第42页 |
| 2.3.3 Characterization of BTME-H-APS stationary phase | 第42-43页 |
| 2.3.4 Column stability evaluation of BTME-H-APS column | 第43-44页 |
| 2.3.5 Chromatographic evaluation of BTME-H-APS column | 第44页 |
| 2.3.6 Applications of BTME-H-APS column | 第44-45页 |
| 2.4 Preparation of Amino/Octyl group bi-ligand stationary phase | 第45-49页 |
| 2.4.1 Preparation of OTS, AEAPTMS and OAEPS stationary phase | 第45-46页 |
| 2.4.2 Column stability evaluation of OAEPS column | 第46页 |
| 2.4.3 Chromatographic evaluation of OAEPS column | 第46-47页 |
| 2.4.4 Investigation the RPLC/IEC retention behavior of OAEPS column | 第47-49页 |
| Chapter 3 Preparation of Aminopropyl-Functionalized Stationary Phase | 第49-57页 |
| Introduction | 第49页 |
| 3.1 Preparation of APS by impregnation method | 第49-52页 |
| 3.1.1 Effect of APTMS amount | 第50-51页 |
| 3.1.2 Effect of reaction time | 第51页 |
| 3.1.3 Effect of Soxhlet extraction progress | 第51-52页 |
| 3.2 Characterization of APS | 第52-53页 |
| 3.2.1 FT-IR | 第52页 |
| 3.2.2 13CNMR | 第52-53页 |
| 3.2.3 Elemental analysis | 第53页 |
| 3.3 Comparison of APS columns prepared by impregnation and liquid-solidmethod | 第53-54页 |
| 3.4 Conclusions | 第54-57页 |
| Chapter 4 Preparation of Ethane-Bridged Pre-Bonded Amino-FunctionalizedStationary Phase | 第57-77页 |
| Introduction | 第57-58页 |
| 4.1 Preparation of BTME-H-APS stationary phase | 第58-60页 |
| 4.1.1 The effect of BTME amount | 第58-59页 |
| 4.1.2 The effect of hydrolysis time | 第59-60页 |
| 4.2 Characterization of BTME-H-APS stationary phase | 第60-63页 |
| 4.2.1 FT-IR | 第60-61页 |
| 4.2.2 ~(13)C CPMAS NMR | 第61页 |
| 4.2.3 ~(29)Si NMR | 第61-62页 |
| 4.2.4 Elemental analysis | 第62-63页 |
| 4.3 Column stability evaluation of BTME-H-APS column | 第63-66页 |
| 4.4 Chromatographic evaluation of BTME-H-APS column | 第66-72页 |
| 4.4.1 Retention properties in RPLC/HILIC mixed-mode | 第66-68页 |
| 4.4.2 Retention properties in NPLC mode | 第68-69页 |
| 4.4.3 Retention properties in IEC mode | 第69-72页 |
| 4.5 Applications of BTME-H-APS column | 第72-74页 |
| 4.5.1 Separation of carbohydrates in honey | 第72-73页 |
| 4.5.2 Separation of alkaloids | 第73-74页 |
| 4.6 Conclusions | 第74-77页 |
| Chapter 5 Preparation of Amino/Octyl Group Bi-Ligand Stationary Phase | 第77-101页 |
| Introduction | 第77-78页 |
| 5.1 Preparation and characterization of the stationary phases | 第78-79页 |
| 5.2 Stability evaluation of OAEPS column | 第79-81页 |
| 5.3 Chromatographic evaluation of OAEPS column | 第81-85页 |
| 5.3.1 Retention properties in RPLC/HILIC mixed-mode | 第81-82页 |
| 5.3.2 Retention properties in RPLC mode | 第82-84页 |
| 5.3.3 Retention properties in IEC mode | 第84-85页 |
| 5.4 RPLC/IEX chromatographic retention behavior of organic acids on OAEPScolumn | 第85-99页 |
| 5.4.1 Retention of the organic acids on the OAEPS column | 第86-87页 |
| 5.4.2 Retention of the organic acids on the OEAPS columns | 第87-97页 |
| 5.4.3 Relative contributions of reversed-phase, ion-exchange andreversed-phase/ion-exchange cooperative retention to the total retention | 第97-99页 |
| 5.5 Conclusions | 第99-101页 |
| Chapter 6 Conclusions and Outlooks | 第101-103页 |
| 6.1 Conclusions | 第101-102页 |
| 6.2 Outlooks | 第102-103页 |
| References | 第103-112页 |
| Notes on publications and participation in scientific research | 第112-113页 |
| Acknowledgements | 第113页 |
