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ISG15在奶牛早期妊娠诊断中的应用及FSH调控卵丘细胞中HSP70的机制研究

摘要第5-6页
Abstracts第6-7页
Abbreviations第10-11页
0 GENERAL REVIEW OF LITERATURE第11-41页
    0.1. Bovine estrous cycle第11-12页
    0.2. Maternal signaling responses第12-14页
    0.3. IFNT release and its function第14-18页
    0.4. Pregnancy diagnosis technique in cattle and future perspective第18-21页
    0.5. Follicle development第21-28页
    0.6. Oocyte maturation & competence第28-30页
    0.7. Origin of cumulus cells第30-34页
    0.8. Regulation of the HSP70第34-35页
    0.9. Inhibition of Hsp70第35-36页
    0.10. Impact of HS on Cellular Functions第36页
    0.11. Different factors that affect IFNT第36-41页
CHAPTER-Ⅰ:Interferon-Stimulated gene ISG15 and ubiquitination enzymes expression isup-regulated in peripheral blood monocyte during early pregnancy in dairy cattle第41-55页
    1.1. Introduction第41-45页
    1.2. Materials and Methods第45-49页
        1.2.1. Pretreatment of animals and sample collection第45页
        1.2.2. Isolation and cultural of the PBMC's第45页
        1.2.3. In vitro culture第45-46页
        1.2.4. RNA concentration and quality determination第46页
        1.2.5. Target gene selection and primer design第46-47页
        1.2.6. Quantitative PCR on mRNA expression第47页
        1.2.7. Western immunoblot第47页
        1.2.8. Statistical analysis of gene expression data第47-49页
    1.3. Results第49-52页
    1.4. DISCUSSION第52-54页
    1.5. Conclusion第54-55页
CHAPTER-Ⅱ:FSH down-regulated the HSP70 through Interferon-tau after heat stress in bovinecumulus cells第55-72页
    2.1. Introduction第55-58页
        2.1.1. Aim of the study第57-58页
    2.2. Material and methods第58-64页
    2.3. Results第64-69页
    2.4. Discussion第69-71页
        2.4.1. Effect of heat stress on HSP70 mRNA第69页
        2.4.2. Possible correlation between IFNT and HSP70 mechanism of transcription and translation as a response to stress第69-71页
        2.4.3. Hormonal regulation of the IFNT第71页
    2.5. Conclusion第71-72页
References第72-85页
Acknowledgement第85页

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