| Abstract | 第5页 |
| Chapter 1. General introduction | 第8-13页 |
| 1.1 Literature review | 第8-12页 |
| 1.1.1 Ansamitocin | 第8-9页 |
| 1.1.2 Actinosynnema pretiosum | 第9页 |
| 1.1.3 Biosynthesis of ansamitocin P-3 | 第9-11页 |
| 1.1.4 Application of ansamitocin P-3 | 第11-12页 |
| 1.2 Purpose of this study | 第12-13页 |
| Chapter 2 Inexpensive nitrogen and carbon sources for AP-3 production by Actinosynnema pretiosum | 第13-35页 |
| 2.1 Introduction | 第13-15页 |
| 2.2 Material and Methods | 第15-19页 |
| 2.2.1 Microorganism and media | 第15-16页 |
| 2.2.2 Determination of cell growth and ansamitocin P-3 | 第16-17页 |
| 2.2.3 Determination of residual sugar | 第17-18页 |
| 2.2.4 Determination of intracellular protein | 第18-19页 |
| 2.3 Transcriptional analysis by quantitative real-time RT-qPCR | 第19-22页 |
| 2.3.1 Extraction of RNA | 第19-20页 |
| 2.3.2 Reverse transcription of RNA | 第20-21页 |
| 2.3.3 Quantitative real-time PCR (RT-qPCR) assay and data analysis | 第21-22页 |
| 2.4 Results and discussion | 第22-34页 |
| 2.4.1 Effect of different low-cost nitrogen and carbon sources on AP-3 production and DCW | 第22-23页 |
| 2.4.2 Soybean powder as nitrogen source for ansamitocin production | 第23-24页 |
| 2.4.3 Addition of different nitrogen sources inhibited AP-3 production | 第24-25页 |
| 2.4.4 Comparison of the cell growth, production of ansamitocin P-3 and sugar consumption between the two media | 第25-27页 |
| 2.4.5 Response of ansamitocin P-3 biosynthesis related genes transcription to different media | 第27-30页 |
| 2.4.6 Effect of different precursors and vitamins on ansamitocin P-3 production | 第30-32页 |
| 2.4.7 Effect of different carbon sources on AP-3 production and cell growth | 第32-34页 |
| 2.5 Conclusion | 第34-35页 |
| Chapter 3 Statistical medium optimization for production ansamitocin P-3 | 第35-50页 |
| 3.1 Introduction | 第35-38页 |
| 3.1.1 Response surface methodology | 第35-37页 |
| 3.1.2 Box-Behnken Design for optimization | 第37-38页 |
| 3.2 Material and methods | 第38-39页 |
| 3.2.1 Optimization medium components by response surface methodology (RSM) | 第38-39页 |
| 3.3 Results and discussions | 第39-49页 |
| 3.3.1 The first round of RSM by Box-Behnken Design | 第39-41页 |
| 3.3.2 Second round of RSM by Box-Behnken Design | 第41-44页 |
| 3.3.3 Third round of RSM by Box-Behnken Design. Verification of predicted condition | 第44-48页 |
| 3.3.4 Sugar consumption in statistically optimized medium | 第48页 |
| 3.3.5 Effect of addition isobutanol in statistically optimized medium | 第48-49页 |
| 3.5 Conclusion | 第49-50页 |
| Chapter 4 General conclusion and perspectives | 第50-51页 |
| References | 第51-59页 |
| Publication list | 第59页 |
