| 摘要 | 第12-14页 |
| ABSTRACT | 第14-19页 |
| Introduction | 第20-26页 |
| References | 第22-26页 |
| CHAPTER 1: Literature Review | 第26-48页 |
| 1.1 Thermoneutral zone | 第28页 |
| 1.2 Effects of heat stress on physiological parameters | 第28-29页 |
| 1.3 Heat Stress and Feed Intake | 第29-30页 |
| 1.4 Heat stress pathway | 第30-31页 |
| 1.5 Heat stress and animal reproduction | 第31-33页 |
| 1.6 Neuroendocrine system and stress | 第33-34页 |
| 1.7 Effects of heat stress on hormone secretion | 第34-35页 |
| 1.8 Acute and chronic stress | 第35-36页 |
| 1.9 Proteomic and transcriptional response to heat stress | 第36-38页 |
| References | 第38-48页 |
| CHAPTER 2: Effects of thermally induced stress on animal behavior and some physiological parameters | 第48-62页 |
| 1 Introduction | 第49-51页 |
| 2 Material methods | 第51-52页 |
| 2.1 Experimental models and heat treatment | 第51-52页 |
| 2.2 Assessment of hyperthermia and physiological parameters | 第52页 |
| 3 Results | 第52-55页 |
| 3.1 Effect of the HS on animal behavior | 第52-53页 |
| 3.2 Effect of the HS on rectal temperature | 第53-54页 |
| 3.3 Effect of the HS on water and feed intake | 第54-55页 |
| 4 Discussion | 第55-57页 |
| References | 第57-62页 |
| CHAPTER 3: Effects of heat stress on hormone profile and testicular histology in mice model | 第62-82页 |
| 1 Introduction | 第63-64页 |
| 2 Materials and methods | 第64-69页 |
| 2.1 Experimental animals and thermal treatment | 第64-65页 |
| 2.2 Sample collection | 第65页 |
| 2.3 Hormonal analysis | 第65-68页 |
| 2.4 Histological analysis | 第68-69页 |
| 2.5 Statistical analysis | 第69页 |
| 3 Results | 第69-73页 |
| 3.1 Hormonal imbalances caused by HS | 第69-72页 |
| 3.2 Histological disturbances caused by HS in mouse testes | 第72-73页 |
| 4 Discussion | 第73-76页 |
| References | 第76-82页 |
| CHAPTER 4: Proteomic response of mouse pituitary gland to heat stress | 第82-124页 |
| 1 Introduction | 第84-85页 |
| 2 Materials and methods | 第85-91页 |
| 2.1 Animals and thermal treatment | 第85-86页 |
| 2.2 Protein identification and quantification by Isobaric tags for relative and absolutequantitation (iTRAQ) | 第86页 |
| 2.3 Protein Quantification and Digestion | 第86-87页 |
| 2.4 iTRAQ Labeling and Fractionation | 第87页 |
| 2.5 LC-MS/MS Analysis | 第87页 |
| 2.6 Data Analysis | 第87-88页 |
| 2.7 Bioinformatics and Annotations | 第88-89页 |
| 2.8 STRING Analysis | 第89页 |
| 2.9 RNA extraction and real-time RT-PCR | 第89-91页 |
| 2.10 Verification of iTRAQ data by western blot | 第91页 |
| 3 Results | 第91-106页 |
| 3.1 Identification of proteins related to stress responsive | 第91-96页 |
| 3.2 Gene Ontology (GO) analysis of differentially expressed proteins during heat stress | 第96-99页 |
| 3.3 Pathway enrichment | 第99-100页 |
| 3.4 Proteins involved in different metabolism pathways | 第100-102页 |
| 3.5 STRING Analysis | 第102-105页 |
| 3.6 Quantitative Analysis of mRNA Levels | 第105-106页 |
| 3.7 Verification of iTRAQ data by western blot | 第106页 |
| 4 Discussion | 第106-115页 |
| References | 第115-124页 |
| SUMMARY AND CONCLUSION | 第124-127页 |
| Acknowledgement | 第127-128页 |
| Dedication | 第128页 |
