| Abstract | 第1-9页 |
| 摘要 | 第9-11页 |
| Abbreviations | 第11-13页 |
| 1. Introduction | 第13-31页 |
| ·Citrus overview | 第13-14页 |
| ·Citrus and tomato anatomical structure | 第14-15页 |
| ·Tomato as a model system to examine the fruit ripening processes | 第15页 |
| ·AP2/ERF transcription factors | 第15-17页 |
| ·The role of super family AP2/ERF in fruit ripening | 第16页 |
| ·DREB (dehydration responsive element binding) role in fruit ripening | 第16-17页 |
| ·The behavior of fruit ripening process in climacteric and non climacteric fruits | 第17-22页 |
| ·Primary metabolism and fruit ripening | 第22-28页 |
| ·The relation between ethylene production and metabolism process during fruit ripening in both climacteric and non-climacteric fruits | 第28-31页 |
| 2. Material and methods | 第31-55页 |
| ·Materials | 第31页 |
| ·Molecular cloning of CgDREB gene | 第31-36页 |
| ·Plant transformation and regeneration | 第36-37页 |
| ·Molecular analysis of transgenic plants | 第37-46页 |
| ·DNA Extraction | 第37-38页 |
| ·PCR test | 第38页 |
| ·RNA isolation and cDNA synthesis | 第38-39页 |
| ·Semi -quantitative real-time RT-PCR | 第39-40页 |
| ·Southern blot analysis | 第40-46页 |
| ·Bioinformatics Analysis | 第46页 |
| ·Subcellular localization assay of CgDREB | 第46-48页 |
| ·GFP vector construct | 第46-48页 |
| ·Experimental design | 第48页 |
| ·Tomato fruit flesh and peel metabolites extracting for GC-MS | 第48-49页 |
| ·Ethylene measurement | 第49页 |
| ·Buffer and solution components: | 第49-55页 |
| ·Componen of the plasmid extraction solutions | 第49-50页 |
| ·Components of Agarose and SDS-PAGE Gel | 第50-51页 |
| ·Southern blot solutions | 第51-54页 |
| ·CTAB buffer | 第54-55页 |
| 3. RESULTS | 第55-71页 |
| ·Isolation and characterization of CgDREB from Citrus grandis. Hirado Buntan Pummelo (HBP) fruit | 第55页 |
| ·Over-express of CgDREB in tomato plant | 第55-60页 |
| ·Subcellular localization and expression pattern | 第60-62页 |
| ·Transgenic plants 35S-CgDREB has a light fruit weight | 第62-63页 |
| ·Changes in organic acids accumulation in CgDREB overexpressed tomato fruits in nesh and peel | 第63-64页 |
| ·Changes in sugars, sugar alcohol and sugar derivatatives and other components accumulation in CgDREB overexpressed tomato fruits in flesh and peel | 第64-68页 |
| ·Changes in fatty acid accumulation in CgDREB tomato fruit in flesh and peel | 第68-69页 |
| ·Fruit ethylene production rate in transgenic lines and wild type | 第69-71页 |
| 4. Discussion | 第71-77页 |
| ·CgDREB characterization, overexpression and phylogenetic analysis | 第71-72页 |
| ·The changes in organic acid in fruit flesh and peel during tomato fruit ripening | 第72-73页 |
| ·The pattern changes in sugars, sugar alcohol and sugar derivatatives accumulation in CgDREB overexpressed tomato fruits in flesh and peel | 第73-74页 |
| ·The Pattern changes in lipids accumulation in CgDREB overexpressed tomato fruits in flesh and peel | 第74-75页 |
| ·The Pattern changes in ethylene production between CgDREB transgenic tomato and wild type | 第75-77页 |
| Conclusion and Future Perspective | 第77-79页 |
| References | 第79-103页 |
| Annexes | 第103-119页 |
| Acknowledgements | 第119-120页 |
