| DEDICATION | 第5-12页 |
| 摘要 | 第12-15页 |
| ABSTRACT | 第15-21页 |
| ABBREVIATIONS | 第22-25页 |
| INTRODUCTION | 第25-30页 |
| RESEARCH OBJECTIVES | 第30-32页 |
| CHAPTER 1 Possible role of rutin and thyroid hormones in testes under restraint stress Literature Review | 第32-66页 |
| 1.1 Stress and endocrine response | 第32-35页 |
| 1.2 The Hypothalamus-Pituitary-Testis system | 第35-37页 |
| 1.3 Male Reproductive System | 第37-40页 |
| 1.3.1 Testes | 第38-39页 |
| 1.3.2 Overview of testis structural organization | 第39-40页 |
| 1.4 Rutin | 第40-45页 |
| 1.4.1 Pharmacological activities of rutin | 第41-42页 |
| 1.4.2 Antioxidant activity of rutin | 第42-45页 |
| 1.5 Thyroid Gland | 第45-55页 |
| 1.5.1 Structure of thyroid gland | 第47-48页 |
| 1.5.2 Microanatomy | 第48-49页 |
| 1.5.3 Function of thyroid hormones | 第49-51页 |
| 1.5.4 Hormones production | 第51-52页 |
| 1.5.5 Regulation | 第52-53页 |
| 1.5.6 Thyroid diseases | 第53-55页 |
| 1.6 Role of thyroid hormone in testicular development | 第55-62页 |
| 1.6.1 The role of thyroid hormone in Sertoli cell proliferation and functional maturation | 第55-57页 |
| 1.6.2 Thyroid hormone and the mechanisms involved in Sertoli cell proliferation | 第57-58页 |
| 1.6.3 The role of thyroid hormone in Leydig cell differentiation and function | 第58-62页 |
| 1.7 Thyroid hormone effects on the adult testis | 第62-66页 |
| CHAPTER 2 Protective effects of rutin on endocrine, histoarchitecture, histopathology and spermatogenesisagainst restraint stress in testes of adult mice | 第66-90页 |
| 2.1 Introduction | 第68-69页 |
| 2.2 Materials and methods | 第69-72页 |
| 2.2.1 Animals | 第69页 |
| 2.2.2 Experimental design | 第69-70页 |
| 2.2.3 Restraint stress protocol | 第70-71页 |
| 2.2.4 Measurement of body weight, food consumption, and water intake | 第71页 |
| 2.2.5 Measurement of testes, epididymis, Adrenal glands, thymus and lipid weights | 第71-72页 |
| 2.2.6 Radioimmunoassay (RIA) for serum hormone concentrations | 第72页 |
| 2.2.7 Histological analysis | 第72页 |
| 2.2.8 Statistical analysis | 第72页 |
| 2.3 Results | 第72-80页 |
| 2.3.1 Feed and water consumption in experimental groups | 第72-73页 |
| 2.3.2 Effects of stress on body weight, testes weight and epididymis weight | 第73-74页 |
| 2.3.3 Effects of stress on adrenal glands, thymus and lipid weights | 第74-75页 |
| 2.3.4 Effects of stress on Serum hormone concentrations | 第75-77页 |
| 2.3.5 Effects of restraint stress on histology of testis | 第77-80页 |
| 2.4 Discussion | 第80-90页 |
| Chapter 3 Protective effects of rutin on caspase-dependent pathway of apoptosis against restraint stress intestes of adult mice | 第90-106页 |
| 3.1 Introduction | 第91-94页 |
| 3.2 Materials and methods | 第94-97页 |
| 3.2.1 Animals | 第94-95页 |
| 3.2.2 Experimental design | 第95-96页 |
| 3.2.3 Restraint stress protocol | 第96页 |
| 3.2.4 Immunohistochemical analysis | 第96-97页 |
| 3.3 Results | 第97-101页 |
| 3.3.1 Effects of restraint stress on immunohistochemical staining of PARP1 and cleavedPARP1 in the testes of mice | 第97-99页 |
| 3.3.2 Effects of restraint stress on immunohistochemical staining of Caspase-3 and cleavedCaspase-3 in the testis of mice | 第99-101页 |
| 3.4 Discussion | 第101-106页 |
| Chapter 4 Protective effects of rutin on sperm count, motility, viability and sperm morphology againstrestraint stress in testes of adult mice | 第106-122页 |
| 4.1 Introduction | 第107-110页 |
| 4.2 Materials and methods | 第110-111页 |
| 4.2.1 Animals | 第110页 |
| 4.2.2 Experimental design | 第110-111页 |
| 4.2.3 Restraint stress protocol | 第111页 |
| 4.3 Sperm Analysis | 第111-113页 |
| 4.3.1 Sperm preparation | 第111-112页 |
| 4.3.2 Sperm count | 第112页 |
| 4.3.3 Sperm motility | 第112页 |
| 4.3.4 Sperm viability | 第112-113页 |
| 4.3.5 Sperm abnormality | 第113页 |
| 4.4 Results | 第113-116页 |
| 4.4.1 Effects of stress on sperm counts, motility, viability and morphological spermabnormalities | 第113-116页 |
| 4.5 Discussion | 第116-122页 |
| Chapter 5 Impaired growth performance and testicular cells apoptosis following restraint stress in adulthypothyroid mice | 第122-144页 |
| 5.1 Introduction | 第123-127页 |
| 5.2 Materials and methods | 第127-130页 |
| 5.2.1 Animals | 第127页 |
| 5.2.2 Experimental design | 第127-128页 |
| 5.2.3 Restraint stress procedure | 第128-129页 |
| 5.2.4 Measurement of body weight, food consumption, water intake and gonadosomaticindex | 第129页 |
| 5.2.5 Radioimmunoassay (RIA) for serum hormone concentrations | 第129页 |
| 5.2.6 Histological analysis | 第129页 |
| 5.2.7 Statistical analysis | 第129-130页 |
| 5.3 Results | 第130-135页 |
| 5.3.1 Feed and water intake | 第130-131页 |
| 5.3.2 Body weight, testes weight and gonadosomatic index | 第131-132页 |
| 5.3.3 Serum hormone concentrations | 第132-133页 |
| 5.3.4 Histological observations | 第133-135页 |
| 5.4 Discussion | 第135-144页 |
| Conclusions | 第144-146页 |
| Innovation | 第146-148页 |
| References | 第148-172页 |
| ACKNOWLEDGEMENT | 第172-174页 |
| PUBLICATIONS | 第174页 |
