| ABSTRACT | 第8页 |
| Abbreviations | 第9-10页 |
| CHAPTER Ⅰ INTRODUCTION | 第10-12页 |
| CHAPTER Ⅱ LITERATURE REVIEW | 第12-17页 |
| 2.1 Reproduction Trait | 第12-13页 |
| 2.2 Milk Production Trait | 第13页 |
| 2.3 RFLP | 第13-14页 |
| 2.4 SNP Association Studies in Reproduction and Production Trait in Dairy Cow and Water Buffalo | 第14-15页 |
| 2.5 Candidate Gene Approach | 第15-17页 |
| 2.5.1 FBXL13 (F-box and leucine-rich repeat protein 13) Gene | 第15-16页 |
| 2.5.2 ARID4B (AT Rich Interactive Domain 4B) Gene | 第16页 |
| 2.5.3 LOC518224 Gene | 第16-17页 |
| CHAPTER Ⅲ MATERIAL AND METHOD | 第17-23页 |
| 3.1 Material Research | 第17-19页 |
| 3.1.1 DNA samples | 第17页 |
| 3.1.2 Equipment and supplies | 第17-18页 |
| 3.1.3 Reagents and solutions | 第18-19页 |
| 3.2 Method | 第19-23页 |
| 3.2.1 Blood sample | 第19页 |
| 3.2.2 DNA extraction | 第19-20页 |
| 3.2.3 SNP detection and genotyping | 第20-21页 |
| 3.2.4 Statistical analizes | 第21-23页 |
| CHAPTER Ⅳ RESULTS AND DISCUSSION | 第23-38页 |
| 4.1 Polymorphism of FBXL13, ARID4B, LOC518224 Candidate Gene in Dairy Cows and Water Buffaloes | 第23-28页 |
| 4.1.1 Polymorphism of FBXL13 candidate gene in Dairy Cows | 第24-25页 |
| 4.1.2 Polymorphism of FBXL13 candidate gene in Water Buffaloes | 第25页 |
| 4.1.3 Polymorphism of ARID4B candidate gene in Dairy Cows | 第25-26页 |
| 4.1.4 Polymorphism of ARID4B candidate gene in Water Buffaloes | 第26页 |
| 4.1.5 Polymorphism of LOC518224 candidate gene in Dairy Cows | 第26-28页 |
| 4.2 FBXL13 Candidate Gene Effects in Reproduction and Milk Production Traits on Dairy Cows | 第28-31页 |
| 4.2.1 Analyze of FBXL13 candidate gene effects in reproduction traits on Dairy Cows | 第28-29页 |
| 4.2.2 Analyze of FBXL13 candidate gene effects in milk production traits on Dairy Cows | 第29-31页 |
| 4.3 ARID4B Candidate Gene Effects in Reproduction and Milk Production Traits onDairy Cows | 第31-34页 |
| 4.3.1 Analyze of ARID4B candidate gene effects in reproduction traits on Dairy Cows | 第31-32页 |
| 4.3.2 Analyze of ARID4B candidate gene effects in milk production traits on Dairy Cows | 第32-34页 |
| 4.4 LOC518224 Candidate Gene Effects in Reproduction and Milk Production Traitson Dairy Cows | 第34-36页 |
| 4.4.1 Analyze of LOC518224 candidate gene effects in reproduction traits on Dairy Cows | 第34-35页 |
| 4.4.2 Analyze of LOC518224 candidate gene effects in milk production traits on Dairy Cows | 第35-36页 |
| 4.5 FBXL13, ARID4B, LOC518224 Candidate Gene Effects in Reproduction and Milk Production Traits on Water Buffaloes | 第36-38页 |
| CHAPTER Ⅴ DISCUSSION | 第38-43页 |
| CHAPTER Ⅵ CONCLUSION | 第43-44页 |
| REFERENCES | 第44-49页 |
| ACKNOWLEDGEMENTS | 第49-50页 |
